ABSTRACT
Background: Lipase enzyme has wide application in industries, particularly food and detergent, but high production cost has always limited its use. Extensive studies are underway on production of high quality and low cost lipase enzyme in large amounts, for which microbial sources have been found to be the best. Aim: To estimate the potential of oil cakes for bacterial lipase production. Methodology: By-products of different oil seeds viz. neem, sesame, flax, mustard, coconut, castor, and groundnut were used for the preparation of fermentation media to culture lipolytic Pseudomonas aeruginosa. Optimization of growth condition was done with respect to different parameters such as fermentation time, nitrogen supplements, carbon additives, and lipid sources. Results: A good lipolytic P. aeruginosa JCM5962 (T) strain was isolated from soil of sugarcane field. Results of the study showed that coconut, sesame, neem, flax and mustard oilcakes induced good lipolytic activity from bacteria. Negligible lipase activity was obtained when organism was cultured in castor and groundnut oilcake medium. 1% ammonium nitrate as an additional nitrogen supplement was found to be ideal parameter for improved production. Conclusion: According to present work, lipases could be economically produced by P. aeruginosa using low cost oil cakes as potent substrate for fermentation medium.
ABSTRACT
The influence of various oil cakes has been investigated for high level production of lipase using Aspergillus tamarii MTCC 5152. By solid state fermentation in wheat bran containing 2.5% w/w gingili oil cake at 70% v/w moisture content the fungus produced a maximal yield of lipase (758 ± 3.61 u/g) after 5 days of incubation using 2% v/w inoculum containing 10(6) spores/mL. Wheat bran and gingili oil cake with supplementation of gingili oil (1.0% w/w), glucose (0.5% w/w) and peptone (0.5% w/w) gives an increased enzyme production of 793 ± 6.56 u/g. The enzyme shows maximum activity at pH 7.0, temperature 50 °C and was stable between the pH 5.0-8.0 and temperature up to 60 °C. Crude lipase (3%) applied to tannery fleshing shows 92% fat solubility. The results demonstrate that fat obtained from tannery fleshing, a by-product of the leather industry has a high potential for biodiesel production and the proteinaceous residue obtained can be used as animal feed.