ABSTRACT
Chronic intestinal pseudo-obstruction (CIPO) is caused by a plethora of primary and secondary causes, dominantly involving the neuromuscular tissue, interstitial cells of Cajal, or the connective tissue framework. The lack of the connective tissue framework, known as desmosis, is evaluated by Masson's trichrome (MT) or picrosirius red stains, both of which are recommended in the London classification. We evaluated the orcein stain in detecting desmosis in comparison to the MT stain. We performed both orcein and MT stains in six previously published cases of complete or partial desmosis along with six age-matched controls. Our results showed comparable results of the orcein stain as compared to the MT stain. Additional advantages of lower cost and a clearer background in orcein stain were noteworthy, whereas MT stain can be used for the detection of additional pathology. We believe that orcein stain can be used as a cheap alternative in resource-limited settings.
ABSTRACT
Determination of sex is useful in Forensic Medicine. Barr bodies can be seen hair root sheath cells. The presence of barr bodies indicates that sex of the person is female. They are inactive X chromosome. The Barr bodies are also known as sex chromatin. Hair can present in the crime scene. Hair is trace evidence. The stain used was aceto-orcein. The percentage of barr bodies was found to be 28-49 %. The study was carried out for eight months and it was found that barr bodies persisted for eight months. It can be present anywhere at the crime scene. Hair of the accused can be present in hands of victim due to cadaveric spasm. Hair of victim can be found on the clothes of the accused. There is average fall in percentage of barr bodies with the passage of time due to the start of decomposition changes in the root sheath cells.