ABSTRACT
Pesticides are major contaminating chemicals in agriculture environment and a hazard to exposed population. The pesticides form a strong class of environment pollutants, as they are sometimes nonbiodegradable, damaged not only the environment and agriculture but also have entered into the food chain thereby affecting health and development. Methyl parathion is a broad-spectrum organophosphorus insecticide generally used to control a variety of insects. The present study was planned to develop a new method for analysis of Methyl parathion in human blood samples using thin layer chromatography technique, which is simple and quick. Methyl Parathion was extracted from blood using solvent extraction methods and then identified on the TLC plates. For chromatographic separation, various binary and tertiary solvent systems were used and for detection on developed plates, palladium chloride reagent was used which successfully increased the sensitivity without dispensing with the simplicity of the method. Statistical analysis was performed on four solvent systems namely benzene: chloroform (80:20), hexane: carbontetrachloride (50:50), hexane: carbontetrachloride (20:80), hexane: propanol (20:80) which included the calculation of mean Rf value, value of standard deviation and coefficient of variance. It is evident from the statistical data that hexane: carbontetrachloride (50:50), hexane: carbon tetrachloride (20:80) is preferably good solvent systems for parathion identification by thin layer chromatography.
ABSTRACT
Malathion is a broad-spectrum Organophosphorus insecticide generally used to control a variety of insects. Routinely it is analysed by Gas Liquid Chromatography & High Performance Liquid Chromatography. Now an attempt has been made to develop a new method for analysis of Malathion in biological samples using Thin Layer Chromatography (TLC) technique, which is inexpensive, accurate and non-destructive. Malathion was extracted from blood and urine using solvent extraction methods and then identified on the TLC plates. For chromatographic separation, various binary and tertiary solvent systems were used to demonstrate in the laboratory. For detection on developed plates, palladium chloride reagent was used which successfully increased the sensitivity without dispensing with the simplicity of the method. For the analysis, a total of 20 solvent systems were chosen. Out of these 20 solvent systems, the three best solvent systems namely Cyclohexane: Chloroform (60:40), Hexane: Acetone (80:20) and Acetone: Hexane: Toluene (40:40:20) were chosen for statistical analysis which included the calculation of mean Rf value, value of standard deviation and coefficient of variance. The method developed is simple, inexpensive, accurate and non-destructive that allows for sensitive and reproducible analysis of Malathion.