ABSTRACT
Under stimulation of PHA,PHA + TPA,or PHA + PTS the time ke-netics of DNA,RNA,protein synthesis and lymphokine secretion by human lymph node cell expressed different responses. The PTS did not increase the DNA and RNA synthesis, but increased the protein synthesis ( 60% ) . TPA increased the RNA synthesis ( 18% ) , therefor increased the protein synthesis ( 40% ) . Both of TPA and PTS increased the production of 5 kinds of lymphokines (IL-1 , IL-2 , IL-3, BCGF, TFNr). We infer that PTS and TPA increase the lymphokine production through different gene regulation. TPA Promotes the translation of lymphokine mRNA。
ABSTRACT
The RNA dot hybridization with human IL-6 cDNA showed that panaxatriol saponin (PTS) enhanced the interleukine-6(IL-6) mRNA concentration within PHA activated peripheral blood mononuclear cell (PBMC) byabout 4 folds,suggesting that PTS could promote the IL-6 gene transcription of lymphoid tissue