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Objective To explore the mechanism of ginsenoside-Rh2(G-Rh2) on inhibition of glioma by identifying differential proteins with proteomic technique. Methods The total proteins were extracted from SHG-44 cells treated with 32 ?mol?L-1 G-Rh2 for 72 h and the cells in control group,then were subjected to two-dimensional gel electrophoresis.Only spots with a fold change equal or above 1.5 and P
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Objective To investigate the inhibitory effect of ginsenoside Rg3 on herpes simplex virus(type 1(HSV-1) in vitro and) its immunoregulative effect.Methods Crystal violet staining was used to observe the inhibitory effect of Rg3 on the cytopathic effect caused by HSV-1.MTT colorimetry was used to detect the influence of Rg3 on the proliferation of Vero cells and mouse splenocytes.The mouse splenocytes were induced with Rg3,and the activity of IL-2 was detected by lymphoblast proliferation assay and IFN-? by cytopathic effect inhibition.(Results The) A_(570) values of cytopathic effect in the experimental group were significantly higher than those in the control group when doses of Rg3 were 1.56-25 mg?L~(-1) in vitro(P0.05).The secretary levels of IL-2 and IFN-? in experimental group were significantly higher than those in the control group in vitro when doses of Rg3 were(3.13-6.25)mg?L~(-1) (P
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Objective To compare the inhibitory effects of ginsenoside-Rh_2,elemene and adriamycin(AMD) on glioma cells cultivated in vitro.Methods MTT was used to detect the inhibitory effects of ginsenoside-Rh_2,elemene and AMD on glioma cell lines(C6,SHg-44,U251).The survival rate-time curves were established.The abilities of three drugs to induce apoptosis of three cells lines were determined by flow cytometry.(Results The) inhibitory effects of the three drugs on the growth of C6,SHg-44 and U-251 were dose-dependent and could be enhanced with dose increasing.The inhibitory effects of Rh_2 was stronger than that of AMD and elemene on three glioma cell lines.The inhibitory concentration of Rh_2 on three cell lines was significantly lower than that of AMD and elemene(P
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Objective To observe the effect of panax quinquefolium fruit saponin(PQFS) on hemodynamics and correlative indexes in the coronary artery ligated dogs.Methods The dogs were divided into five groups randomly(n=6): control group,gingkgo leaf tablet group(24 mg?kg~(-1)),24,12 and 6 mg?kg~(-1) PQFS groups.The myocardial infarction size(MIS)and the changes of the serum enzymes were determined by using the acute myocardial infarction models with the ligation of left anterior descending coronary artery of dogs.The parameters of hemodynamics and myocardial oxygen metabolism were measured in the anesthetic dogs with thoracotomy.(Results Compared) with control group,the myocardial blood flow was increased(P
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Objective To study the direct inhibition of Ginsenoside-Rg3(GS-Rg3) on human keloid fibroblasts cultivated in vitro and its possible mechanism.Methods The effects of GS-Rg3 with different concentrations(25,50,100 and 200 mg?L~(-1)) on proliferation activity of fibroblast hyperplasia at different time were detected by viable count and MTT chromometry.Results Compared with control group,GS-Rg3 inhibited the proliferation of keloid fibroblasts.The inhibition increased with the prolongation of time and the increasing of concentration.And the max inhibitory concentration was 100 mg?L~(-1).Conclusion Gs-Rg3 can inhibit the proliferation of human keloid fibroblasts cultivated in vitro.And the inhibition will increase with the prolongation of time and the increasing of concentration.
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A gravimetric method for determining the total saponin content in Xuesuantong capsule has been developed. The proposed method has the advantages of efficiency, simplicity and accuracy. The average recovery rate is 97.6％ with a relative standard deviation of 0.78％. AHhough colorimetrg has been employed commonly, there were many factors affecting colour reaction, so thatthe result was not ideal. Now the gravimetric method is applied to control the quality temporarily during the period of clinisal study.
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Radix Ginseng and Rhizoma Chuanxiong were identified and the content of ginsenoside Rg_1 was determined by TCL scanning. The results showed that the content of ginsenoside Rg_1 ranged between 0.01770％ and 0.01826％, the recovery rate of ginsenoside Rg_1 was 99.94％ and the coefficient of variation derived from samples was 1.659％. This method is found to be stable, accurate and reliable.