ABSTRACT
Aim To investigate the underlying pharmacological mechanism of Tibetan medicine Byur dMar 25 (BM25) in the treatment of Alzheimer's disease(AD) based on network pharmacology approaches. Methods The potential target of the main blood components after oral administration of BM25 was predicted through reverse pharmacophore mapping scheme, and the main biological functions and cell signaling pathways were systematically analyzed based on bioinformatics strate-gies. Results BM25, as a multi-component drug with numerous potential targets may mainly participate in MAPK, insulin, mTOR signaling pathways, contributing to different biological functions including inflammation, apoptosis, and expression and clearance of beta amyloid ( A{3). Conclusion BM25 could interfere the progress of Alzheimer's disease, and the mechanism is a concerted pharmacological intervention of multiple components and targets.
ABSTRACT
AIM: To investigate the effects of estrogen interference on ab dominal adipose accumulation in C7BL/6J mice. METHODS: Estrogen was administrated to the ovariectomized mice (estrogen-deprived model) and the changes of abdominal adipose weight of the mice in the normal estrous cycle and pro-menopausal were observed. Mice of 3 months old were randomly divided into c ontrol, ovariectomized (OVX), OVX+estrogen 15-days (E d 15 ) and OVX+ estrog en 60-days (E d 60 ) groups. Each group had 10 mice. Mice were raised and sa crificed until 7 months old after ovariectomized. 5 months and 10 months old mic e were divided into control, estrous cycle normal + estrogen 60 days, pro-menop ausal and pro-menopausal+60 days groups. 17?-estradiol time-release pellets ( 0.18 mg/pellet) were given ic in 15 days and 60 days before mice w ere sacrificed. RESULTS: The abdominal adipose weight in OVX gro up increased significantly (P 0.05 ). There was no difference in the abdominal adipo se weight between control and estrous cycle normal + estrogen 60-days groups, a nd between pro-menopausal and pro-menopausal+ estrogen 60-days groups. CONCLUSION: Ovariectomy significantly influences the metabolism of abd ominal adipose that causes adipose accumulation. Estrogen interference, especial ly long-term administration, can attenuate the abdominal adipose accumulation. Exogenous estrogen interference has no effect on abdominal adipose accumulation on cycle normal and pro-menopausal mice.