Fatores associados a condição física ativa em pacientes com hipertensão arterial sistêmica: Um estudo transversal / Factors associated with active physical condition in hypertensive patients: A cross sectional study

O exercício físico é uma intervenção não farmacológica que pode colaborar para o controle dos valores pressóricos em pacientes hipertensos, sendo este recomendado por diretrizes internacionais e nacionais. Entretanto, a adesão de pacientes hipertensos a programas de exercício físico ainda está longe do ideal. Assim, o objetivo do presente estudo foi determinar os fatores que podem influenciar um estilo de vida ativo em pacientes hipertensos. Participaram do estudo oitenta e sete (87) indivíduos diagnosticados clinicamente com hipertensão arterial sistêmica que aceitaram participar de forma voluntária. Os pacientes foram submetidos a dois questionários que abordavam as características socioeconômicas, demográficas, tempo de diagnóstico da doença, conhecimento sobre a ação benéfica do exercício físico na hipertensão arterial sistêmica; bem como caracterizavam o nível de atividade física dos pacientes (IPAq). A partir dos resultados do IPAq, os pacientes foram dicotomizados em ativos ou sedentários. O teste de qui-quadrado demonstrou associação significante entre a condição física e o fator idade, sexo, tempo de diagnóstico de hipertensão arterial sistêmica e ser estimulado a praticar exercícios físicos por um profissional da área da saúde. Após a inserção dos fatores significantes na regressão logística binária univariada, os resultados demonstraram que os fatores: idade maior que 55 anos (OR bruta=2,27), ser estimulado por um profissional da área da saúde, (OR bruta=0,169) e ser homem (OR bruta=2,95) eram preditores da condição física ativa do paciente hipertenso. Por sua vez, a análise multivariada demonstrou que apenas os fatores idade (OR ajustada=12,43) e ser estimulado por um profissional da área da saúde a praticar exercício físico (OR ajustada=-2,21) continuavam preditores da condição hipertensa ativa. Os dados do presente estudo apresentam indícios que pacientes hipertensos com mais de 55 anos que são estimulados por profissionais da área da saúde a praticar exercícios físicos apresentam mais chance de serem fisicamente ativos.(AU)

Physical exercise is a non-pharmacological approach recommended by national and international guidelines from health care associations, to collaborate with blood pressure control in hypertensive people. However, hypertensive patients adherence to physical exercise programs is far from the ideal. Thus, the aim of the present study is determine the factors associated with active physical condition in hypertensive patients. Eighty-seven (87) clinically diagnosed patients with hypertension were recruited to participate in this study. Patients were underwent to two questionnaires. The first-one recorded socio economic and demographic characteristics, time since diagnosis and knowledge about the beneficial effects of physical exercise in hypertension; The second questionnaire dichotomized hypertensive people to active or sedentary state (IPAq). Since the classification of physical state, the chi-square test showed significantly association between physical condition and age, gender, time since diagnosis and be encouraged to practice physical exercises by a health professional. Univariate binary logistic regression showed that be older than 55 year (unadjusted OR =2.27), be encouraged to practice physical exercises by a health professional, (unadjusted OR =0,169) and be a man (unadjusted OR =2.95) were predictor factors of the active physical condition in hypertensive patients. In turn, multivariate binary logistic regression showed age (adjusted OR =12, 43) and be encouraged to practice physical exercises by a health professional (adjusted OR =-2.21) as the significantly predictor factor in active hypertensive state. Data of the present study evidenced that hypertensive patients older than 55 years, which are encouraged to practice physical exercises by a health professional, present higher odds ratio to be active physically.(AU)

The physical states of HPV DNA and expression of human papillomavirus late capsid protein 1 in HepG2 cells / 基础医学与临床

Objective To find out the physical state of the human papillomavirus ( HPV) genome in hepatoma cell line HepG2 cells and the regulation of HPV late capsid protein 1 ( L1) expression and to explore the nature of the cytoryctes in HepG2 cells.Methods E2 and E6 in HPV18 were detected by PCR to evaluate the physical state of HPV18 genome .HepG2 L1 expression was detected by ELISA , light microscropy and electron microscrope immu-nohistochemistry assays , Western blot assay using HPV L 1 mice monoclonal antibody .L1 mRNA in HepG2 cells was detected by reverse transcriptional PCR ( RT-PCR) .Results PCR assay displayed that HPV DNA was inte-grated with HepG2 genome.ELISA assay showed that HPV L1 was present in lysate of HepG2 cells.Light micros-cropy demonstrated strong positive reaction in HepG2 cells.In microscopy, in the cytoplasm of partial HepG2 cells, there were lumpish cytorrhyctes materials which consists of very small and uniform particles and these parti -cles were marked by HPV L1 antibody labeled by colloidal gold .Western blot analysis showed a band at 56 ku dis-trict and it was L1 specific strap which demonstrated HPV 18 L1 was present in HepG2 cells.RT-PCR assay demon-strated the presence of L1 mRNA in HepG2 cells.Conclusions HepG2 cells are HPV18-positive HPV DNA ge-nome is integrated with HepG2 cells.HepG2 cells can express L1.The cytorrhyctes in HepG2 cells are composed of HPV18 L1 indicating that L1 can be expressed in HepG2.