ABSTRACT
Objective To explore the clinical effect of pituitrin and oxytocin in laparoscopy myomectomy of intramural fibroids.Methods Sixty patients received laparoscopy myomectomy of intramural fibroids during October 2015-December 2016 were selected and divided into two groups with 30 cases in each.We applied injections of pituitrin 6IU + 10ml normal saline to treatment group while 20U oxytocin + 10ml normal saline to control group so as to compare their blood pressure change and other situations based on medication.Results Compared with control group,the time of operation and amount of bleeding during operation in treatment group reduced obviously(98.13 ± 5.07min vs 110.83 ± 8.03min,t =-7.324,P =0.000).The hemoglobin changed before and after operation (67.50 ±10.24ml vs 97.83 ± 16.10ml,t =-8.705,P =0.000).15min after medication during operation,the systolic pressure in treatment group increased obviously,which was higher than that in control group(150.77 ±:5.57mmHg vs 142.03 ± 5.21mmHg,t =6.273,P =0.000).Conclusion The application of pituitrin to laparoscopy myomeetomy of intramural fibroids was better hemostatic effect than oxytoein,but it may geuerate temporary elevation of blood pressure,so the clinical application should combine with patients' individual situation.
ABSTRACT
Aim To observe the preventive protection of Yingxindan on acute myocardial ischemia induced by sublingual injection of pituitrin ( pit) in rats.Methods Sixty rats were randomly divided into saline group , model group, nifedipine 12.6 mg · kg -1 group, and Yingxindan 25.2, 12.6, 6.3 mg · kg -1 groups.The saline group and model group were given the same vol-ume of water.After administration for 7 d, an acute myocardial ischemia model was induced by sublingual intravenous injection of pit 1 U · kg -1 in the rats 1 h after last administration .The changes of the II electro-cardiogram(ECG) ST segment at different time points , as well as the positive rate of myocardial ischemia and arrhythmia were observed .Meanwhile , the levels of serum creatine kinase ( CK ) , creatine kinase isoenzyme ( CK-MB ) , glycogen phosphorylase isoenzyme BB ( GPBB ) , cardiac troponin I ( cTnI ) and cardiac troponin T ( cTnT ) were observed .Re-sults Yingxindan could suppress the changes of ST segment of electrocardiogram in rats with acute myocar-dial ischemia induced by pit and reduce the positive rate of myocardial ischemia and arrhythmia .The con-tents of myocardial injury markers CK , CK-MB, GPBB, cTnI and cTnT in serum significantly de-creased, and the corresponding relationship existed . The greater the dose of Yingxindan , the smaller the content of myocardial injuried markers in serum .Con-clusion Yingxindan has significant preventive protec-tion effect on acute myocardial ischemia by pit .
ABSTRACT
Objective To explore the clinical efficacy and safety of pituitrin and phentolamine in the treatment of senile bronchiectasis with massive hemoptysis,thus to provide guidance for the clinical.Methods 80 elderly patients were selected as the subjects of this experiment,and the patients were randomly divided into two groups using the envelope random grouping method.40 patients in the control group used phentolamine.40 cases in the observation group were treated with pituitrin and phentolamine.The clinical curative effect,bleeding time,bleeding volume and the incidence of adverse reactions were compared between the two groups.Results The total effective rate of the observation group was 92.50％,which was significantly higher than 70.00％ of the control group (x2 =6.646,P ＜ 0.05).The bleeding time,total amount of bleeding of the observation group were significantly less than those of the control group (tbleeding time =5.069,tbleeding volurne =5.447,all P ＜ 0.05).The incidence rate of adverse reaction in the observation group was 7.50％,which was significantly lower than 27.50％ of the control group (x2 =5.541,P ＜ 0.05).Conclusion Phentolamine combined with pituitrin in the treatment of senile bronchiectasis with massive hemoptysis can significantly relieve symptoms,shorten the bleeding time,reduce the total amount of bleeding,reduce the probability of adverse reactions in patients.
ABSTRACT
Objective To explore the curative effect of pituitrin in laparoscopic conservative surgery in the treatment of 60 patients with tubal pregnancy.Methods 120 eggs tube pregnancy patients were selected as the research subjects.According to the random blind points method,they were divided into the observation group and control group,60 cases in each group.The control group was undergoing laparoscopic conservative surgery directly,the observation group was injected pituitrin in the mesosalpinx before laparoscopic surgery.The intraoperative and postoperative condition,postoperative hospitalization days and blood β-human chorionic gonadotrophin(β-HCG) level changes and clinical curative effect were observed and compared in the two groups.Results The intraoperative blood loss,hemostatic time,operation time,postoperative body temperature,hospitalization days of the observation group were (21.12±7.86)mL,(3.61±1.68)min,(35.23±10.45)min,(37.35±0.65)℃,(5.64±0.66)d,respectively,which of the control group were (97.63±12.11)mL,(13.21±2.91)min,(67.97±20.13)min,(37.62±0.48)℃,(6.75±0.75)d,respectively,the differences between the two groups were statistically significant(t=41.049,22.130,11.181,2.588,8.606,all P<0.05).The serum β-HCG at 1d,4d,7d after operation of the observation group were (587.54±105.14)U/L,(141.28±41.21)U/L,(52.47±22.32)U/L,respectively,which of the control group were (609.45±114.89)U/L,(238.43±52.16)U/L,(115.33±26.15)U/L,respectively,the serum-HCG values of the two groups were lower than before operation,there were significant difference between the two groups in serum-HCG after operation at 4d and 7d(t=11.320,14.162,all P<0.05).The effective rate of the observation group was 98.33%,which of the control group was 86.67%,and the difference between the two groups was statistically significant(χ2=5.885,P<0.05).Conclusion The laparoscopic surgical treatment of tubal pregnancy patients with preoperative injection of pituitrin can not only reduce the bleeding in the operation and shorten the operation time and hospital stay,lower blood β-HCG level,also can improve the clinical curative effect,it is worthy of popularization and application.
ABSTRACT
Objective A novel Active Pharmaceutical Ingredient (API) pituitrin efficacy component content analysis method was explored, and the method is conformed to the requirements of the pharmaceutical production enterprise testing standard. Methods The reverse phase high performance liquid chromatography (RP-HPLC) was performed on a Zorbax Eclipse XDB-C18(250mm×4.6mm, 5 μm) (P/N 993967-902/ 5063-6600) Column, the column temperature was 25℃, the wavelength of detector was set at 220 nm, flow rate was 1.2 mL/min, 50% Acetonitrile- 0.13mol/L sodium dihydrogen phosphate aqueous solution was used as mobile phase for gradient elution. 20 μL sample solution was injected in each perform. Results The content of oxytocin and vasopressin as pituitrin efficacy components were analyzed by this method with advantage of simple and easy operation, good reliability and high precision. Different biological extraction technology process A and B was used on pituitrin injection production, the content of medicinal ingredients in the product: oxytocin and vasopressin is slightly different, but concentration level is different, which process B concentration is higher than that of A. Conclusion In this study, an effective determination the levels of oxytocin and vasopressin in pituitrin API method was established for a pharmaceutical production enterprise, can provide API pituitrin purification process control. Relevant technical information can be provided in the above, which worked on the biological API pituitrin research and development.
ABSTRACT
Objective To study the protective effect of Xuemailing on pituitrin-induced myocardial rats. Methods The rats were divided into 7 groups(by means of random number table), 10 rats in each group, namely blank control group, model control group,Di'aoxinxuekang group and experimental groups(Xuemailing prepared fleece flower root high and low dose groups;Xuemailing raw radix polygoni multiflori high and low dose groups), each group was ig administrated once every day for 7 days, Blank control and model control groups were given the same volume of purified water.Effects of 7 groups on T wave and S-T segment were observed,LDH,CK,SOD,MDA activities in serum of rats were determined. Results High dose Xuemailing (prepared fleece flower root) significantly decreased the elevation of 10,30,45 s,1 min ST segment and 10,30,45 s T wave in the myocardial ischemia rat model.Low dose Xuemailing (prepared fleece flower root) significantly decreased the elevation of 10 s,1 min ST segment and 10 s T wave of the rat model.High dose Xuemailing(raw radix polygoni multiflori) significantly decreased the elevation of 10 s ST segment and 10,45 s T wave in the rat model.Xuemailing (prepared fleece flower root) and Xuemailing (raw radix polygoni multiflori) significantly decreased LDH,CK,MDA enzyme activities,and increased SOD enzyme activities of the myocardial ischemia rat model. Conclusion Xuemailing can protect the myocardial ischemia rats induced by pituitrin.
ABSTRACT
The present study was designed to evaluate the cardioprotective effect of latifolin on pituitrin(Pit) or isoproterenol(ISO)-induced myocardial injury in rats, and further investigate its underlying mechanisms. Rats were administrated sublingually with pituitrin or subcutaneously with isoproterenol to induce acute myocardial ischemia in rats, and lead II electrocardiograph was recorded. In rats with isoproterenol, ELISA assay or colorimetric method was used to detect the content or activity of myocardial injury markers in serum, and the SOD activity and MDA content in myocardium were detected by colorimetric assay; histopathological examination was conducted by HE staining; the frozen section of myocardial tissues was used for DCFH-DA fluorescent staining to detect the content of ROS in myocardium; Western blot was used to detect the protein expression levels of Nrf2, Keap1, HO-1 and NQO1 in myocardium. Results showed that latifolin significantly inhibited ST-segment changes induced by pituitrin or isoproterenol, and increased heart rate. Further mechanism study showed that latifolin reduced cardiac troponin I(cTnI) level, aspartate transaminase(AST) and lactate dehydrogenase(LDH) activities in serum, increased myocardial superoxide dismutase(SOD) activity and reduced myocardial malondialdehyde(MDA) level, and protected myocardium with less necrosis, infiltration of inflammatory cells and fracture of myocardial fibers. Furthermore, latifolin obviously reduced ROS level in myocardium, inhibited the expression of Kelch-like ECH-associated protein-1(Keap1), increased the nuclear translocation of nuclear factor erythroid 2 related factor 2(Nrf2), and promoted the expression of Heme oxygenase-1(HO-1) and NAD(P)H quinone oxidoreductase-1 (NQO1) in myocardial tissues. Our data suggest that latifolin has a potent protective effect against pituitrin or isoproterenol-induced myocardial injury, which may be related to inhibition of oxidative stress by activating Nrf2 signaling pathway.
ABSTRACT
Objective:To investigate the protective effects and possible mechanisms of cortex phellodendri water extract and etha -nol extract on the myocardial injury induced by pituitrin and isoproterenol hydrochloride in rats .Methods:SD rats as the experimental animals were randomly divided into the normal control group , model group , compound Danshen tablets group , phellodendron water ex-tract group and phellodendron ethanol extract group .Pituitrin and isopropyl adrenaline hydrochloride were used to establish the myocar-dial injury model in rats.The serum CK, LDH activity, myocardial tissue SOD activity and MDA content were detected and compared . Results:Compared with those in the normal control group , the serum LDH activity , CK activity and MDA content were significantly in-creased , and the SOD activity in cardiac muscle and myocardial tissue was significantly decreased in the pituitrin -established myocardi-al injury model group (P<0.01).In the isopropyl adrenaline hydrochloride-established myocardial injury model group , the MDA con-tent in myocardial tissue was obviously increased , and the SOD activity in myocardial tissue was decreased obviously (P<0.01).The serum LDH activity, CK activity and MDA content were significantly decreased , and the SOD activity in cardiac muscle and myocardial tissue was increased significantly in all drug-taken groups when compared with those in the pituitrin-established myocardial injury model group, and the differences were statistically significant (P<0.05 or P<0.01).The MDA content in myocardial tissue was significant-ly reduced , and the SOD activity was increased significantly in all drug-taken groups when compared with those in the isopropyl adrena-line hydrochloride-established myocardial injury model group , and the differences were statistically significant (P<0.05 or P<0.01). Conclusion:Cortex phellodendri extract has certain protective effect on myocardial injury induced by pituitrin and isopropyl adrenaline hydrochloride in rats .
ABSTRACT
Objective:To provide reference for clinical pharmacists through pharmaceutical care for patients with hyponatremia en-cephalopathy caused by pituitrin. Methods: Clinical pharmacists provided pharmaceutical care for one patient with hyponatremia en-cephalopathy caused by pituitrin. It was suggested by clinical pharmacists that 3% hypertonic saline and 20% mannitol be used in the patient with sodium ions and renal function monitoring. The patient was also told to increase salt in diets and limit water uptake. Re-sults:The ion levels of the patient returned to normal after the pharmaceutical care. Conclusion:Clinical pharmacists play an impor-tant role in the prevention and treatment of hyponatremia encephalopathy caused by pituitrin.
ABSTRACT
Objective To investigate clinical effects of laparoscopic salpingotomy combined with pituitrin in the treatment of tubal pregnancy.Methods 160 children with tubal pregnancy were chosen in our hospital in the period and randomly divided into two groups including control group (80 children)with laparoscopic salpingotomy treatment and treatment group (80 children)with pituitrin adjunct treatment before operation on the basis of control group.And the operation time,intraoperative bleeding volume,postoperative body temperature,hospitalization time,the retention rate of fallopian tube,fallopian tube patency rate,persistent ectopic pregnancy rate,intrauterine pregnancy rate and fertility rate of both groups were compared.Results The operation time,intraoperative bleeding volume, postoperative body temperature and hospitalization time of the control group were separately (20.33 ±2.70)min, (52.76 ±7.42)mL,(37.52 ±0.54)℃ and (7.56 ±1.98)d;the operation time,intraoperative bleeding volume, postoperative body temperature and hospitalization time of the treatment group were separately (8.94 ±1.61)min, (16.33 ±2.15)mL,(37.46 ±0.50)℃and (5.91 ±1.42)d;the operation time,intraoperative bleeding volume and hospitalization time of the treatment group were significantly better than those of the control group (t=2.26,2.60, 2.08,all P0.05).The retention rate of fallopian tube and fallopian tube patency rate of the control group were sepa-rately 75.00% (60/80)and 52.50%(42/80);the retention rate of fallopian tube and fallopian tube patency rate of the control group were separately 93.75% (75/80)and 81.25% (65/80);the retention rate of fallopian tube and fallopian tube patency rate of treatment group were significantly better than those of the control group (χ2 =9.71, 10.14,all P0.05);the intrauterine pregnancy rate and fertility rate of treatment group were significant-ly better than those of the control group (χ2 =11.84,12.52,all P<0.05).Conclusion Laparoscopic salpingotomy combined with pituitrin in the treatment of tubal pregnancy can efficiently decrease operation time the amount of bleeding,shorten hospitalization time and be helpful to improve the reproductive outcome.
ABSTRACT
OBJECTIVE:To systematically review the efficacy and safety of pituitrin combined with phentolamine versus pitui-trin alone in the treatment of bronchiectasis hemoptysis(BH),and provide evidence-based reference for the clinical treatment. METHODS:Retrieved from PubMed,Cochrane Library,EMBase,CJFD,Wanfang and VIP database,the randomized controlled trails(RCT)about pituitrin combined with phentolamine(test group)versus pituitrin alone(control group)in the treatment of BH were collected,and afterdata extract and quality evaluation ,Meta-analysis was performed by using Rev Man 5.2.3 statistics soft-ware. RESULTS:A total of 43 RCTs were included,involving 3 094 patients. Results of Meta-analysis showed the significant effi-cacy [RR=1.50,95%CI(1.38,1.62),P<0.001] and effective rate [RR=1.25,95%CI(1.20,1.30),P<0.001] in test group were sig-nificantly higher than control group,inefficacy [RR=0.29,95%CI(0.23,0.36),P<0.001], hemoptysis stopped or remission time [MD=-2.00,95%CI(-2.43,-1.57),P<0.001],incidence of headache [RR=0.36,95%CI(0.22,0.59),P<0.001],incidence of chest tightness [RR=0.41,95%CI(0.26,0.63),P<0.001],incidence of abdominal pain [RR=0.26,95%CI(0.16,0.43),P<0.001] and incidence of elevated blood pressure [RR=0.28,95%CI(0.14,0.56),P<0.001] in test group were significantly lower than control group,there were significant differences in 2 groups. CONCLUSIONS:Pituitrin combined with phentolamine has better efficacy and safety than pituitrin alone in the treatment of BH. Due to the limit of methodological quality and sample size,it re-mains to be further verified with more rigorously designed and long-term follow-up of large-scale RCT.
ABSTRACT
Objective To compare and analyze the clinical curative effect of pituitrin and oxytocin applying in laparoscopic uterine myomectomy intraoperation .Methods According to surgical hemostasis method ,68 patients with uterine fibroids were divided into pituitrin group (group A) and oxytocin group(group B).The surgery time,intr-aoperative blood loss ,the change of postoperative hemoglobin levels and the change of blood pressure before and after treatment were observed.Results In A group,the operative time was (69.8 ±18.0)min,blood loss was (68.3 ± 19.1)mL,postoperative discharge time was (15.2 ±7.8)h,preoperative and postoperative hemoglobin difference val-ue was (10.4 ±2.6) g/L,which were significantly better than those in group B [(82.8 ±17.6) min,(88.5 ± 25.6)mL,(19.4 ±5.9)h,(13.5 ±2.9)g/L],the differences were statistically significant (all P0.05).In A group,the systolic blood pressure was (152.0 ±8.5)mmHg after treatment for 15min,which was significantly higher than (132.5 ±10.2)mmHg in group B,the difference was statistically significant (P0.05).Conclusion Application of pituitrin in laparoscopic surgical hemostasis for patients with uterine fibroids can effectively reduce the surgery time ,decrease the blood loss ,its surgical hemostasis effect is better than oxytocin ,which is worth further clinical application .
ABSTRACT
Objective To investigate the value of Pituitrin in neonatal cardiopulmonary resuscitation.Methods Seventy-three cases with neonatal cardiopulmonary arrest admitted in emergency department and NICU in our hospital were collected during 2007 to 2011.Newborns who did not respond to conventional neonatal resuscitation therapy were divided into two groups:epinephrine group 47 cases (control group) and Pituitrin combined with epinephrine group 26 cases (treatment group).Results There were no statistical difference (x2 =0.956,P ＞ 0.05) between treatment group and control group in the rates of initial resuscitation success (23.1％,6/26 vs 34.0％,16/47).Conclusion Pituitrin combined with epinephrine has similar efficacy with the use of epinephrine in neonatal cardiopulmonary resuscitation.
ABSTRACT
Objective To explore the clinical effect of octreotide combined with propranlolum in the treatment of portal hypertension combined upper alimentary canal haemorrhage.Methods 63 cases with portal hypertension upper gastrointestinal hemorrhage were randomly divided into two groups,both of the two groups were given same basic medical care,while then one group was treated with octreotide combined with propranlolum,and the other group was given pituitrin.Results There was statistical difference in defecate occult blood disappear time,total effective rate between treatment group and control group (P ＜ 0.05).Conclusion Octreotide combined with propranlolum was more effective to the patients with portal hypertension upper gastrointestinal hemorrhage than pituitrin.
ABSTRACT
Objective To evaluate the effect of pituitrin and nursing for patients with vasoplegic syndrome after cardiopulmonary bypass. Methods 38 patients with cardiopulmonary bypass after open heart operation were divided into two groups with 19 cases in each group according to odd-nunbered and even-numbered days, the PT group was given pituitrin treatment, the NE group was given norepinephrine treatment. Heart rate, MAP, urine volume were recorded at 0.5, 1, 2, 6, 12 and 24 hours after drug therapy.Mechanical ventilation time, ICU stay time,hepatic and renal function and clinical symptom were compared between the two groups. Results MAP and urine volume increased significantly at 0.5 hour after pituitrin therapy. MAP increased from (55.33±4.10)mmHg to (67.34±4.13)mmHg. Urine volume increased from (0.08±0.02)ml·kg-1·h-1 to (1.84±1.21)ml·kg-1 ·h-1 MAP and urine volume also increased at other time points. In the NE group, MAP increased at 1h after treatment. Both MAP and urine volume increased at 2h and later after norepinephrine treatment. One death happened in the NE group. Compared with the NE group, the PT group was associated with lower ICU stay, mechanical ventilation time and 24h serum LAC.Liver and kidney function, myocardial infarction, nausea and vomiting, and other side effects were not different between the two groups. Conclusions Application of low-dosage pituitrin is rapid, efficient and safe treatment for patients with vasoplegic syndrome after open heart operation.
ABSTRACT
Objective To compare the curative effect and untoward effect of EGVB treatment with four different methods. Methods The clinical information of 160 EGVB patients were analyzed,40 cases were treated by octreotide combined with glonoin( A group) ,40 cases were treated by octreotide( B group) ,40 cases were treated by octreotide combined with pituitrin ( C group) , 40 cases were treated by pituitrin combined with glonoin ( D group). Results Curative effect and average hemostasis time of A and C group were better than B and D group, average transfusion of A and C group were less than B and D group, untoward effect incidence rate of A and B group were less than C and D group. Conclusion Octreotide combined with glonoin have satisfied curative effect for EGVB patients, and had less untoward effect,it was worth generalizing.
ABSTRACT
[Objective] To observe the effect of HQSM on PGI_2/TXA_2 in blood plasma of acute myocardial ischemia rats caused by injecting pituitrin. [Methods] 84 SD rats were divided into seven groups at random: normal control group,model control group,three HQSM groups(210mg/kg,150mg/kg,105mg/kg),Huangqi Shengmaiyin and DI’ao Xinxuekang. Intragastric administration was adopted once a day for 30 days.Acute myocardial ischemia model rat was established by injecting pituitrin from tail vein 1h after the last administration, and blood plasma was separated to be determined the activity of 6-Keto-PGF_ 1? and TXB_2, which is the metabolite of PGI_2 and TXA_2 in blood plasma of SD rats. [Results] Having been injected pituitrin,the activity of TXB2 upgraded while 6-Keto-PGF_ 1? /TXB_2 decreased(P
ABSTRACT
Objective To compare and analyze the hemostases effects,contraindication and side effects of procaine and pituitrin in the treatment for pulmonary tuberculosis secondary middle and large haemoptysis of the age.Methods The observation group:to mix procaine(300mg) with glucose(5%) for intravenous drip,15～20gtt/min twice a day.It was maintained 72 hours after the haemoptysis stops.The comparative group:to mix pituitrin(10～20U) with glucose(5%) for intravenous drip,15～20gtt/min twice a day.It was maintained 72 hours after the haemoptysis stops.Results The procaine group had a better hemostatic effect than the pituitrin group;While the pituitrin group had more tangible contraindication and side effects.Conclusion Procaine is more suitable than pituitrin for the treatment for pulmonary haemoptysis of the aged.
ABSTRACT
Objective To summarize the clinical experience of serious hyponatremia resulted from applying pitnitrin to massive hemorrhage of gastrointestinal tract patients.Methods we used pitritrion on 2460 such patients and examined some symptoms of the digestive tract and nerve system,monitoring the dynamic state of blood sodium.Results The re- suits reveal that patients in 30 cases turned out to suffer from serious hyponatremia,whese sodium in the serum appeared less than 110 mmol/L,which was 1.22% of the rest subjects'.The relevant symptoms included nausea,vomit,spoor,coma and twitch etc.Following remedies included liquid supply with a high-concentrated sodium,ceasing using pituitrin,in- take of salute foods,and dynamic monitoring on the state of sodium in the serum.All patients recovered in 1 to 3 days without a single failure.Conclusion Thus we reach this conclusion:When applying pituitrin to massive hemorrhage of gastrointestinal tract patients,the above mentioned symptoms should be closely examined,and blood sodium should be timely monitored so that proper treatment should arrive in time.
ABSTRACT
Objective:To study the protective e ect of Sini decoction on experimental myocardial ischemia in rats.Methods:The model of acute myocardial ischemia in rats was induced by subcutaneous injection of isoprenaline and sublingual venous injection of pituitrin.The changes of ECG,serum activity of creatine phosphokinase(CPK),lactate dehydrogenase(LDH),aspartate aminotransferase(AST),malondialdehyde(MDA) and superoxide dismutase(SOD)were measured.Results:Sini decoction signi cantly improved the extents of abnormal rising and lowering of ST segment(P