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OBJECTIVE:To establish a method for the identification of Chongqing local medicinal material Heracleum hemsley-anum. METHODS:The original plant morphology,morphological characteristics,microscopic characteristics (transverse section and powder),were identified. RESULTS:H. hemsleyanum had cone shape root,brown,many-branched with special fragrance;the microscopic characteristics of taproot a transverse showed no differences with lateral root transverse section,primary xylem was di-arch,secondary phloem was more developed than secondary xylem,secondary phloem was rich in the oil chamber. Vascular tissues contained a large number of parenchyma cells. In TLC chromatrogram,the test sample showed the same color fluorescent spots with-the substance reference of osthole and columbianadin in the corresponding locations. CONCLUSIONS:The established method is simple with high accuracy,and can be used as the basis for identification of H. hemsleyanum.
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Objective: To clarify the origin of Ficus hirta and to control the quality of the crude drug. Methods: The reference on F. hirta, the microscopic features of powder and tissue of the crude drug, plant morphology, and phytochemical TLC were observed and studied. Results: The result of study provides the using history and experiment research of F. hirta and characteristics of the crude drug, which could be used for the identification of the crude drug and Chinese patent medicine. Conclusion: The convenient and effective method for identification of crude drug and patent drug has been established, which provides the basis for quality control.
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Aims: This study is developed to discuss whether the plant morphology and seed characters (macro and micromorphological and protein pattern) can provide an additional fundamental tool helping in explanation of the taxonomic trends at specific and infraspecific level within the 47 studied taxa belonging to subfamily Mimosoideae (Fabaceae) and to compare the proposed taxonomic treatment based on numerical analysis (dendrogram) with other previous and current systems of classification. Methodology: The macro and micromorphological characters of the whole plant and seed as well as seed protein pattern of 47 taxa of subfamily Mimosoideae, family Fabaceae were investigated (using LM, SEM and Stereomicroscope and SDS-PAGE technique respectively). The taxa under investigation represent three tribes, seven genera and 46 species including three subspecies. The macro and micromorphological criteria (219 attributes) and seed protein pattern attributes (38 bands) extracted were numerically analyzed using NTsys-Pc program (version 2.02). Results: The taxonomic treatment of the Mimosoideae taxa under investigation were based on the numerical analysis of 257 macro-, micromorphology of whole plant and seed protein attributes. The dendrogram interprets the similarities and dissimilarities between the investigated taxa. The dendrogram revealed that the taxa under investigation were split into two main series and 25 groups. The splitting Series I includes 12 groups which represented by 20 of the studied Acacia species. Series II includes 13 groups which represented by 15 sp. of Acacia and the species of other six studied genera, group 17 as well as group 21 contain species from different genera. The specific and infra-specific relationships were discussed and compared with some current systems of classification. Conclusion: There is no difference of opinion about the phyletic position of the Acacieae which is always considered a link between Mimoseae and Ingeae. However different affinities of the genus Acacia taken as a natural unit have been suggested. The tribe Mimoseae represents the polymorphic and older core of Mimosoideae.
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Begonia x elatior plantlets which regenerated from leaf disk callus showed variations in plant morphology, number of flowers per plant, and flower size. Variations in flowering period, number of flowers per plant, and flower morphology were observed in Saintpaulia ionantha L. plants directly regenerated from leaf disk explants. The cytokinins, benzylaminopurine and zeatin, tested in the culture medium did not affect the basic plant characteristics including flower colour which remained stable in both species. Micropropagation of selected somaclones having the desirable trait of high number of flowers per plant was stable in the MV2 and MV3 generations.
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In this article we first report the original plant morphology of padicularis decora, Its histological features of the root, diagnostic characters of the Crude drug and the identification tests of physical chemistry. It is clear that our vesearch also provides the basis for the appraisal of the new resources of medicinal herbs.