ABSTRACT
Objective to investigate the level of platelet leukocyte aggregates in patients with acute cerebral infarction and their short term prognosis.Methods 105 patients with acute cerebral infarction onset within 24 hours were selected continuously,then platelet leukocyte aggregates including neutrophil aggregates (PNA) and platelet monocyte aggregates (PMA) and platelet lymphocyte aggregates (PlyA) were detected by flow cytometry within 24 hours of admission and the incidence of 14 days.modified Rankin Scale(mRS) was performed at 14 days of onset,as a prognostic indicator,and the mRS score was good at 3.The score >3 mRS was divided into poor prognosis.The level of platelet leukocyte aggregates was detected in 50 healthy subjects.Results (1) The platelet leukocyte aggregates in patients with acute cerebral infarction group were significantly higher than that of the healthy group,which was statistically significant (P3 score comparison,the difference of white blood cell aggregates was statistically significant(P<0.05).Conclusion leukocyte aggregates could be used as an index of short-term prognosis in patients with acute cerebral infarction.
ABSTRACT
To investigate the relationship between platelet-leukocyte aggregates (PLA) and serum high sensitive C-reactive protein (hs-CRP)in type 2 diabetic patients with retinopathy (DR).The blood levels of platelet-neutrophil aggregates( PNA )and platelet-monocyte aggregates ( PMA ) were determined by flow cytometry and serum hs-CRP by ELISA in 30 patients with proliferative diabetic retinopathy( PDR group),30 patients with no-proliferative diabetic retinopathy( NPDR group),25 diabetic patients without DR( DM group),and 30 normal controls.The levels of PNA,PMA,and hs-CRP in PDR group were significantly higher than those in normal control,NPDR,and DM groups.The levels of PNA,PMA,and hs-CRP in NPDR group were significantly higher than those in normal control and DM group( P＜0.01 or P＜0.05 ).The PNA and PMA were correlated positively with hs-CRP in patients with DR ( r =0.587,0.576 ; P ＜ 0.05 ).The data showed that the levels of PLA and hs-CRP were raised significantly in patients with DR,and the levels of PLA were positively correlated with inflammatory response and the seriousness of DR,suggesting that these findings may play an important role in the development of DR.
ABSTRACT
Objective To research the clinical significance of leukocyte-platelet aggregates(PLA)in patients with acute cerebral infarction.Methods Sixty-five patients with acute cerebral infarction and 24 controls.The levels of PLA,platelet-neutrophil aggregates(PNA),platelet-lymphocyte aggregates(PLyA)and platelet-monocyte aggregate(PMA)were analyzed by two-color flow cytometry,soluble P-selectin(sP-sel)was detected by ELISA,serum C-reaction protein(CRP)was detected by nephelometry and platelet aggregation rate(PAR)was measured by blood agglutination.Results There were no differences in the percentages of PLA,PNA and PLyA between patients(4.12?1.17%,4.09?1.27%,4.28?1.09%)and controls(4.10?0.99%,3.98?1.12%,4.01?1.98%).However,in the patients before treatment,the percentage of PMA(11.54?1.88%)was significantly increased compared with that after treatment(8.40?2.74%,P