ABSTRACT
OBJECTIVE To explore the construction of a pediatric pharmaceutical care cloud platform based on Western Pediatric Development Union， to provide a reference for narrowing the difference of pediatric pharmaceutical care in western regions and medical institutions. METHODS Based on the Western Pediatric Development Union， the “1+3+3” pediatric pharmaceutical care cloud platform had been built by relying on the alliance telemedicine service network. That was， with Children’s Hospital of Chongqing Medical University as one center， three core pharmaceutical services， including prescription dispensing， pharmaceutical guidance and health education， were carried out in the union through standardized 3 aspects of management of resource information， service process and component interface. RESULTS & CONCLUSIONS Established “1+3+ 3” pediatric pharmaceutical care cloud platform based on the remote service network of the Western Pediatric Development Union registered 1 208 thousands registrations since its operation， with 112 thousands online prescriptions and 44 thousands circulation prescriptions completed； the total number of medication consultation cases was 10 694， and the number of online training people exceeded 15 thousands. However， during the operation， there are also problems such as limited clinical medication data and insufficient coverage of the region. With the accumulation of clinical medication data on the platform， the artificial intelligence technology will be used to extract the data of prescriptions， medication behavior， and physical indicators after medication， the correlation analysis of data will be conducted under the conditions of different geographical environments， different age groups， different heights and weights in the region to obtain clinical medication characteristics for children in the region， providing decision support for further guiding rational and safe medication in pediatric clinical practice.
ABSTRACT
Introducción: La Universidad de Ciencias Médicas de Matanzas dispone de un aula virtual soportada por la plataforma Moodle. Su uso, a pesar de las actividades de superación realizadas como talleres, cursos y adiestramientos, continúa siendo un reto para estudiantes y profesores, pues no todos son capaces de explotar las herramientas que brinda esta plataforma en función de cumplir con los objetivos determinados en los programas de estudio de cada asignatura. Objetivo: Valorar el uso que hacen de la plataforma Moodle los estudiantes de la asignatura Enfermería en Urgencias, en la Facultad de Ciencias Médicas de Matanzas. Materiales y métodos: Se realizó un estudio cuantitativo en el período mayo-julio de 2023, para evaluar el uso de la plataforma Moodle que hacen los estudiantes de la asignatura Enfermería en Urgencias en la Facultad de Ciencias Médicas de Matanzas. Resultados: Los resultados mostraron que existió un buen uso de algunas de las herramientas de la plataforma Moodle por parte de los estudiantes, lo que llevó a una alta adquisición de conocimientos. Conclusiones: Sin duda, las nuevas tecnologías pueden suministrar medios para la mejora de los procesos de enseñanza aprendizaje, pero su utilización a favor o en contra de una sociedad más desarrollada, dependerá en gran medida de la educación, de los conocimientos y la capacidad crítica de sus usuarios, que son las personas que ahora se están formando.
Introduction: The Matanzas University of Medical Sciences has a virtual classroom supported by the platform Moodle. Its use, despite the improvement activities carried out, like workshops, courses and trainings continues to be a challenge for students and teachers, since not everyone is capable of exploiting the tools provided by this platform in order to meet the objectives determined in the study programs for each subject. Objective: To assess the use made of the Moodle platform by students of the subject Emergency Nursing, at the Faculty of Medical Sciences of Matanzas. Materials and methods: A quantitative study was carried out in the period May-July2023, to assess the use of the Moodle platform by students of the subject Emergency Nursing at the Faculty of Medical Sciences of Matanzas. Results: The results showed that there was good use of some of the Moodle platform tools by the students, which led to a high acquisition of knowledge. Conclusions: Without a doubt, new technologies can provide means for the improvement of teaching-learning processes, but their use for or against amore developed society will depend to a large extend on the education, knowledge and critical capacity of their users, who are the people who are now being trained.
ABSTRACT
A translação do conhecimento (TC) visa à utilização prática dos resultados de pesquisas científicas e o monitoramento dos benefícios causados à saúde da população. O objetivo deste estudo foi instrumentalizar a instância de suporte à TC por meio da identificação dos requisitos funcionais de uma proposta de plataforma tecnológica. Fundamentando-se no Design Science Research, o estudo se enquadra como uma pesquisa qualitativa procurando resolver um problema prático num contexto específico. Por meio de um grupo focal confirmatório com profissionais e pesquisadores de um instituto de ciência e tecnologia em saúde, foram identificadas 11 categorias de requisitos funcionais: Órgãos Regulatórios, Infraestrutura de Implementação, Banco de Dados, Capacitações em TC, Eventos de TC, Perfil de Consultas, Portal TC, Avaliação de Iniciativas, Apoio Institucional, Ferramenta de Busca Parametrizada e Prospecção de Projetos de Pesquisa. A modelagem da proposta contemplou as sugestões de funcionalidades para instrumentalizar o processo de aplicação prática dos conhecimentos decorrentes das pesquisas
Knowledge translation (KT) aims at the practical use of scientific research results and the monitoring of the benefits caused to the population's health. The objective of this study was to instrumentalize the TC support instance by identifying the functional requirements of a proposed technological platform. Based on Design Science Research, the study is framed as qualitative research seeking to solve a practical problem in a specific context. Through a confirmatory focus group with professionals and researchers from a health science and technology institute, 11 categories of functional requirements were identified: Regulatory Bodies, Implementation Infrastructure, Database, Capacity Building, KT Events, Query Profile, KT Portal, Initiative Evaluation, Institutional Support, Parameterized Search Tool, and Research Project Prospecting. The modeling of the proposal contemplated the suggestions of functionalities to instrumentalize the process of the practical application of the knowledge resulting from the research
La traslación del conocimiento (TC) tiene como objetivo el uso práctico de los resultados de la investigación científica y el seguimiento de los beneficios causados a la salud de la población. El objetivo de este estudio fue instrumentalizar la instancia de apoyo a la TC a través de la identificación de requisitos funcionales de una plataforma tecnológica propuesta. Basado en Design Science Research, el estudio se enmarca como una investigación cualitativa que busca resolver un problema práctico en un contexto específico. A través de un grupo focal confirmatorio con profesionales e investigadores de un instituto de ciencia y tecnología de la salud, se identificaron 11 categorías de requisitos funcionales: Órganos Reguladores, Infraestructura de Implementación, Base de Datos, Capacitación, Eventos de TC, Perfil de Consulta, Portal de TC, Evaluación de Iniciativas, Apoyo Institucional, Herramienta de Búsqueda Parametrizada y Prospección de Proyectos de Investigación. El modelado de la propuesta incluyó las sugerencias de funcionalidades para instrumentalizar el proceso de aplicación práctica del conocimiento surgido de la investigación
Subject(s)
Humans , Technology , Health , Knowledge , Scientific Research and Technological Development , Qualitative ResearchABSTRACT
There are a number of challenges that the travel and tourism sector in India face during post COVID-19 session. Innovations in e- platform are the adoption of infrastructure and information technology in the field of tourism by transforming the e-processes. Thereby it is an alarming time for marketing to attempt to match the hike in expectations of tourists and travelers to achieve effectiveness and efficiency in promotion of the tourism sector. e- Platform is playing an important revolutionized role both in consumer choice and service delivery processes. Due to the impact of Marketing 5.O, customers are becoming much more sophisticated and concerning because they have experienced high levels of service and standards of living as an user with high frequency of e mode in all aspect of marketing activities in recent days. It has been growing considerably due to the availability and penetration of more choices in e platforms in all aspects of marketing activities involved in tourism sector. The aim of this paper is to establish the fact happen in tourism sector due to variables in choices and opinion of tourists and travelers due post COVID session. A structured questionnaire is used to collect responses on e mail and WhatsApp. The survey is designed for 100 respondents and analysis is done by considering research objectives based on innovations and use of e platform in the development of tourism sector
ABSTRACT
@#The COVID-19 pandemic created the urgent need to use online and virtual platforms to facilitate development of skills and competencies as part of remote learning of students. MyDispense®, a learning management system developed by Monash University in Australia, is a practice-based online tool utilized by the University of the Philippines College of Pharmacy to teach dispensing skills in Dispensing and Incompatibilities courses (Phar 154 and Pharm 133). This commentary describes how MyDispense® was used as an online retail pharmacy simulation through the SEIPS (Systems Engineering Initiative for Patient Safety) model, with discussion on how the person, tasks, organization, tools and technology, and virtual environment work within the teaching and learning process in dispensing. The application of MyDispense® as a learning platform both in remote and traditional teaching can be further explored to maximize its features and utility.
ABSTRACT
With the rapid development of healthcare big data and artificial intelligence technology, how to utilize the massive medical data generated based on clinical diagnosis and treatment has become an important issue to be solved in the field of clinical research. Clinical diagnosis and treatment data is an essential part of healthcare big data, and also the main field of healthcare big data research. With the continuous deepening and extensive development of informatization, hospitals have accumulated a large number of patient-centered clinical diagnosis and treatment data. Deeply mining and analyzing these data through big data technology can provide reference for precise diagnosis and treatment, and standardized prevention and control of diseases. However, conducting relevant research still faces many difficulties and blockages, such as the increased risk of data leakage or abuse, and the difficulty in implementing informed consent. To safely, legally and efficiently utilize clinical diagnosis and treatment data to conduct clinical research and fully tap into the value of these precious medical resources, a tertiary hospital in Beijing has built a research big data platform and developed relevant systems to effectively solve the problems of blockages and difficulties in the application of rich clinical resources to clinical research, and improve the service quality of medical institutions and the conversion rate of scientific research achievements. By introducing the key points and management methods in the implementation of clinical research based on the scientific research big data platform, analyzing and exploring the existing problems and improvement measures, this paper aimed to provide theoretical basis and system reference for high-quality and efficient health and medical big data clinical research, inspire and promote the continuous improvement of medical research management, and promote the development of medical and health science and technology innovation.
ABSTRACT
【Objective】 To establish a rare blood group information supply platform in Shaanxi Province. 【Methods】 The rare blood group information supply platform consists of sample registration, result registration, donor files and inventory blood. The blood donation codes of voluntary blood donors were recorded for blood typing, and the antigen identification results of each blood group system were registered, all stored in the rare blood type information supply platform. When receiving an application for unusual or rare blood type missing multiple conventional antigens or a certain high-frequency antigen, the corresponding antigen negative blood donors and their blood status (in stock or not) were queried from the donor profile module of the platform, and the inventory of blood of rare blood type was monitored dynamically. 【Results】 The results showed that 5.060% (273/5 398) of rare Rh phenotype donors, 1.540‰ (51/33 010) of donors lacking multiple regular antigens, and 13 O-type donors lacking high-frequency antigens were recorded in the rare blood type information supply platform. Among them, 0.019‰ (3/158 484) of Jk(a-b-) phenotype, 0.436‰ (2/4 586) of Di(a+b-) phenotype, and 4.030‰ (8/1 983) of Fy (a-b+) phenotype were stored in the blood bank for rare blood type. 【Conclusion】 The establishment of rare blood group information supply platform can meet the urgent demand for blood of rare blood types in clinical practice and ensure the safety of blood transfusion.
ABSTRACT
ObjectiveTo explore the clinical efficacy of three-dimensional motion platform training on balance and walking function of stroke patients. MethodsFrom August, 2021 to August, 2022, 80 stroke patients from Second Affiliated Hospital of Guangzhou Medical University were selected and randomly divided into control group (n = 40) and experimental group (n = 40). The control group received routine rehabilitation training, and the experimental group received three-dimensional motion platform training on the basis of routine rehabilitation training. Before and four weeks after treatment, the Berg Balance Scale (BBS), Functional Ambulation Category (FAC) and 3D gait analysis (step speed, step frequency, percentage of standing phases on the affected side, percentage of double support phase) were used to assess the balance and walking function of patients. ResultsFour weeks after treatment, the scores of BBS, FAC, and step speed, step frequency, percentage of standing phases on the affected side and percentage of double support phase significantly improved in both groups (|t| > 4.423, |Z| > 5.292, P < 0.001), and they were better in the experimental group than in the control group (|t| > 3.748, |Z| = 2.646, P < 0.05). ConclusionThree-dimensional motion platform training could facilitate to improve the balance and walking function of stroke patients.
ABSTRACT
The human skeletal muscle drives skeletal movement through contraction. Embedding its functional information into the human morphological framework and constructing a digital twin of skeletal muscle for simulating physical and physiological functions of skeletal muscle are of great significance for the study of "virtual physiological humans". Based on relevant literature both domestically and internationally, this paper firstly summarizes the technical framework for constructing skeletal muscle digital twins, and then provides a review from five aspects including skeletal muscle digital twins modeling technology, skeletal muscle data collection technology, simulation analysis technology, simulation platform and human medical image database. On this basis, it is pointed out that further research is needed in areas such as skeletal muscle model generalization, accuracy improvement, and model coupling. The methods and means of constructing skeletal muscle digital twins summarized in the paper are expected to provide reference for researchers in this field, and the development direction pointed out can serve as the next focus of research.
Subject(s)
Humans , Technology , Computer Simulation , Databases, Factual , Movement , Muscle, SkeletalABSTRACT
OBJECTIVE@#In order to solve the technical problems, clinical researchers face the process of medical imaging analysis such as data labeling, feature extraction and algorithm selection, a medical imaging oriented multi-disease research platform based on radiomics and machine learning technology was designed and constructed.@*METHODS@#Five aspects including data acquisition, data management, data analysis, modeling and data management were considered. This platform provides comprehensive functions such as data retrieve and data annotation, image feature extraction and dimension reduction, machine learning model running, results validation, visual analysis and automatic generation of analysis reports, thus an integrated solution for the whole process of radiomics analysis has been generated.@*RESULTS@#Clinical researchers can use this platform for the whole process of radiomics and machine learning analysis for medical images, and quickly produce research results.@*CONCLUSIONS@#This platform greatly shortens the time for medical image analysis research, decreasing the work difficulty of clinical researchers, as well as significantly promoting their working efficiency.
Subject(s)
Machine Learning , Diagnostic Imaging , Algorithms , RadiographyABSTRACT
The traditional intravenous infusion configuration in medical institutions has the shortcomings of long time and low efficiency， and it is difficult to ensure that the dispensed liquid medicine is not contaminated. At the same time， medical staff may be exposed to risks such as cytotoxic drugs due to lack of protection. To solve the defects and deficiencies in intravenous infusion configuration， the “Dolphin 7” pharmacy intravenous compounding robots are introduced in our hospital， cluster management platform is constructed based on data middle platform service exchange system； mini-intelligent PIVAS module is designed and constructed. The mode can reduce the workload of medical staff， save medical construction and labor costs， also help to promote and deepen the construction of intelligent hospitals.
ABSTRACT
Biochemistry and Molecular Biology are the cornerstone courses of talent training in the field of life science. Taking these course as an example, this study explored reconstructing the knowledge framework, developing teaching cases, sharing teaching resources, innovating teaching means and establishing ideological education patterns. Supported by the scientific research achievements with discipline characteristics and online teaching platform, this research explored and practiced an integrated curriculum reform mode. This mode is guided by scientific research and education, based on the course development, and driven by communication and cooperation. A shared space of "exchange, practice, openness and informatization" was developed to achieve free and independent integration of undergraduate and graduate teaching motivated by learning knowledge, resulting in an effective student training.
Subject(s)
Humans , Curriculum , Students , Learning , Molecular Biology/education , Biochemistry/educationABSTRACT
Resumo Introdução: o termo pós-Fordismo refere-se a um modelo de organização laboral de acordo com a evolução dos meios de produção na segunda metade do século XX. Fruto das alterações substanciais dos mecanismos de regulação e contratação, este modelo prioriza a flexibilização e fragmentação da relação com o trabalhador. Com o advento informático, o pós-Fordismo ganha novos moldes e hiperboliza-se com as economias de plataforma em linha e a chamada uberização do trabalho. Objetivo: analisar criticamente as características do modelo pós-fordista e seus impactos à saúde ocupacional. Métodos: foi realizada uma revisão narrativa da literatura. Resultados: apontam-se para três aspectos do modelo pós-Fordista: 1) instabilidade laboral; 2) sobrecarga laboral; e 3) demanda psicossocial. Esses determinantes têm sido fortemente associados a maus resultados em saúde, sobretudo mental. Conclusões: as mudanças na conceptualização do trabalhador e do lugar do trabalho na vida do indivíduo exigem novas formas de pensar a saúde ocupacional. Para mitigar as consequências do pós-Fordismo, é necessária uma clara percepção das suas dinâmicas e uma mobilização de esforços multissetoriais.
Abstract Introduction: the term post-Fordism refers to the evolution of labour organization according to the new production models that emerged in the second half of the 20th century. As a result of substantial changes in the mechanisms of regulation and contracting, they prioritize the flexibilization and fragmentation of the employer-employee relationship. With the advent of information technology, post-Fordism gained new moulds and became hyperbolized with the online platform economies and the so-called uberization of work. Objectives: to critically analyse the characteristics of the post-Fordist model and its impacts in occupational health. Methods: this research was carried out with a review of the literature. Results: we point to three aspects of the post-Fordist model: 1) work instability; 2) work overload; and 3) psychosocial demand. The impact of these aspects, determinant on labour activities, have strong associations with bad health outcomes, especially in mental health. Conclusions: these changes in the conceptualization of workers and the place of work in their lives require new ways of thinking about occupational health. A clear perception of its dynamics, and a mobilization of multisectoral efforts, is needed to mitigate the consequences of this model.
ABSTRACT
Resumo O isolamento social provocado pela pandemia do covid-19 acelerou um processo que já estava em curso: a plataformização da economia. Uma das aplicações presentes nesse processo foi a distribuição de comida por motoboys, ocasionando uma transformação radical das condições de trabalho, que necessita ser investigada. O objetivo desta pesquisa foi discutir essas condições e os riscos que representam para a saúde do trabalhador. 14 entregadores de alimentos foram entrevistados, suas falas foram gravadas e transcritas para análise temática, obtendo dois principais resultados: a precarização das condições de trabalho e a vulneração da saúde. A plataforma promete independência e autonomia quanto ao trabalho, mas, ao mesmo tempo, cria insegurança, não garantindo nenhuma proteção trabalhista nem subsistência financeira estável, por não assegurar os meios para o trabalho. Por outro lado, essa precarização do trabalho produz riscos para a saúde. Essa situação demanda uma discussão sobre o amparo legal dos direitos e a proteção da saúde desses trabalhadores.
Abstract The social distancing brought about by the COVID-19 pandemic sped up a process that was already underway: the emergence of the platform economy. One of the applications present in this process was for the delivery of food by motorbike couriers, causing a radical transformation of working conditions that requires investigation. The objective of this study was to discuss those conditions and the risks that they pose to the health of workers. A group of 14 food delivery couriers were interviewed and their statements were recorded and transcribed for thematic analysis, resulting in two main findings: precarious employment conditions and health vulnerabilities. The platform promises independence and autonomy regarding work, but at the same time creates insecurity, failing to ensure work protection or financial stability, as it does not guarantee working conditions. On the other hand, such precarious work generates health risks. This situation requires a discussion on the legal protection of the labor rights and health protection of these workers.
Subject(s)
Right to Work/legislation & jurisprudence , COVID-19ABSTRACT
Aim: The present split-mouth case report aims to describe the clinical and radiographic long-term outcomes of the implant rehabilitation of two mandibular premolars in which the digital workflow was used to apply different prosthetic protocols. Case description: A female 42-year-old patient with the absence of both mandibular second premolars was submitted to guided surgery for the placement of platform-switching Grand Morse connection implants. Digital workflow was used for implant and prosthetic planning, applying early loading protocol 21 days after surgery. The implant on the right side received the final abutment at the time of surgery (without loading), whereas the implant on the left side had a healing abutment placed, which was replaced by a temporary abutment and then by a final abutment. Two months after surgery, both implants had final ceramic restorations delivered. The patient was followed clinically and radiographically for 30 months, presenting excellent hard and soft tissue outcomes, with bone level changes lower than 2mm for both implants. Conclusion: The use of digital workflow and early loading, made the present implant-supported rehabilitation predictable, safe and time-efficient, resulting in total patient satisfaction. Peri-implant bone level was observed to be stable after early loading protocol for both platform-switching connection implants inserted, despite the prosthetic protocol applied.(AU)
Objetivo: O presente relato de caso de boca dividida tem como objetivo descrever os resultados clínicos e radiográficos a longo prazo da reabilitação com implante de dois pré-molares inferiores em que o fluxo de trabalho digital foi usado para aplicar os conceitos de "one abutment-one time" em uma das reabilitações e troca de componente no outro. Descrição do caso: Paciente do sexo feminino, 42 anos, com ausência de ambos os segundos pré-molares inferiores, foi submetida à cirurgia guiada para colocação de implantes de conexão Grand Morse plataforma-switching. Foi utilizado fluxo de trabalho digital para planejamento de implante e prótese, aplicando protocolo de carga antecipada 21 dias após a cirurgia. O implante do lado direito recebeu o componente protético definitivo no momento da cirurgia (sem carga), enquanto o implante do lado esquerdo recebeu um cicatrizador, que foi substituído por um pilar provisório e depois por um componente definitivo. Dois meses após a cirurgia, ambos os implantes tiveram restaurações cerâmicas finais entregues. A paciente foi acompanhada clínica e radiograficamente por 30 meses, apresentando excelentes resultados de tecidos duros emoles, com alterações do nível ósseo inferiores a 2mm para ambos os implantes. Conclusão: O fluxo de trabalho digital e carregamento precoce, tornou a presente reabilitação implantossuportada previsível, segura e eficiente em termos de tempo, resultando em total satisfação do paciente. O nível ósseo peri-implantar foi observado como estável após o protocolo de carregamento inicial para ambos os implantes de conexão plataforma-switching inseridos, independente do protocolo protético aplicado. (AU)
Subject(s)
Humans , Female , Adult , Prostheses and Implants , Dental Implants , Alveolar Bone Loss , Patient Satisfaction , Computer-Aided DesignABSTRACT
OBJECTIVE@#To analyze the cement flow in the abutment margin-crown platform switching structure by using the three-dimensional finite element analysis, in order to prove that whether the abutment margin-crown platform switching structure can reduce the inflow depth of cement in the implantation adhesive retention.@*METHODS@#By using ANSYS 19.0 software, two models were created, including the one with regular margin and crown (Model one, the traditional group), and the other one with abutment margin-crown platform switching structure (Model two, the platform switching group). Both abutments of the two models were wrapped by gingiva, and the depth of the abutment margins was 1.5 mm submucosal. Two-way fluid structure coupling calculations were produced in two models by using ANSYS 19.0 software. In the two models, the same amount of cement were put between the inner side of the crowns and the abutments. The process of cementing the crown to the abutment was simulated when the crown was 0.6 mm above the abutment. The crown was falling at a constant speed in the whole process spending 0.1 s. Then we observed the cement flow outside the crowns at the time of 0.025 s, 0.05 s, 0.075 s, 0.1 s, and measured the depth of cement over the margins at the time of 0.1 s.@*RESULTS@#At the time of 0 s, 0.025 s, 0.05 s, the cements in the two models were all above the abutment margins. At the time of 0.075 s, in Model one, the gingiva was squeezed by the cement and became deformed, and then a gap was formed between the gingiva and the abutment into which the cement started to flow. In Model two, because of the narrow neck of the crown, the cement flowed out from the gingival as it was pressed by the upward counterforce from the gingival and the abutment margin. At the time of 0.1 s, in Model one, the cement continued to flow deep inside with the gravity force and pressure, and the depth of the cement over the margin was 1 mm. In Model two, the cement continued to flow out from the gingival at the time of 0.075 s, and the depth of the cement over the margin was 0 mm.@*CONCLUSION@#When the abutment was wrapped by the gingiva, the inflow depth of cement in the implantation adhesive retention can be reduced in the abutment margin-crown platform switching structure.
Subject(s)
Finite Element Analysis , Cementation/methods , Gingiva , Crowns , Dental Abutments , Dental Cements , Dental Stress AnalysisABSTRACT
Objective: To analyze the expression levels of the F9 gene and F9 protein in hepatocellular carcinoma by combining multiple gene chip data, real-time fluorescence quantitative PCR (RT qPCR), and immunohistochemistry. Additionally, explore their correlation with the occurrence and development of hepatocellular carcinoma, as well as with various clinical indicators and prognosis. Methods: The mRNA microarray dataset from the GEO database was analyzed to identify the F9 gene with significant expression differences associated with hepatocellular carcinoma. Liver cancer and adjacent tissues were collected from 18 cases of hepatocellular carcinoma. RT-qPCR method was used to detect the F9 gene expression level. Immunohistochemistry was used to detect the F9 protein level. Combined with the TCGA database information, the correlation between F9 gene expression level and prognostic and clinicopathological parameters was analyzed. The biological function of F9 co-expressed genes associated with hepatocellular carcinoma was analyzed by the Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). Statistical analysis was performed using Graphpad Prism software. Results: Meta-analysis results showed that the expression of the F9 gene was lower in HCC tissues than in non-cancerous tissues. Immunohistochemistry results were basically consistent with those of RT-qPCR. The data obtained from TCGA showed that the F9 gene had lower expression values in stages III-IV, T3-T4, and patients with vascular invasion. A total of 127 genes were selected for bioinformatics analysis as co-expressed genes of F9, which were highly enriched in redox processes and metabolic pathways. Conclusion: This study validates that the F9 gene and F9 protein are lower in HCC. The down-regulation of the F9 gene predicts adverse outcomes, which may provide a new therapeutic target for HCC.
Subject(s)
Humans , Carcinoma, Hepatocellular/pathology , Liver Neoplasms/pathology , Down-Regulation , Prognosis , Gene Expression , Gene Expression Regulation, NeoplasticABSTRACT
ObjectiveTo explore the mechanism of Tangbikang granules （TBK） against diabetic peripheral neuropathy （DPN） based on network pharmacology and in-vivo experiment. MethodThe active components in medicinals of TBK and their target genes were searched from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform （TCMSP）. The active components of the medicinals which are not included in TCMSP were searched from previous research. After the analysis of drug-likeness by SwissADME， the target genes of them were predicted with SwissTargetPrediction. DPN-related target genes were retrieved from GeneCards. The common targets of the disease and the prescription were the hub genes of TBK against DPN， which were uploaded to Metascape for Gene Ontology （GO） term enrichment and Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway enrichment analysis. High-sugar and high-fat diet and low-dose streptozotocin （STZ， ip） were employed to induce diabetes in rats， and then the model rats were respectively treated with low-dose （0.625 g·kg-1）， medium-dose （1.25 g·kg-1）， and high-dose （2.5 g·kg-1） TBK for 12 weeks. Sensory nerve conduction velocity （SNCV） was evaluated. After hematoxylin and eosin （HE） staining， the sciatic nerve was observed under light microscope to examine the nerve damage. Real-time PCR was performed to detect the gene expression of adenosine monophosphate-activated protein kinase （AMPK） pathway-related targets in rat sciatic nerve， and Western blot to measure the protein expression of AMPK and phosphorylated （p）-AMPK in rat sciatic nerve. ResultThe main active components of TBK， such as quercetin， kaempferol， β-sitosterol， leech pteridine A， stigmasterol， and baicalein were screened out， mainly acting on interleukin-6 （IL-6）， tumor necrosis factor （TNF）， protein kinase B （Akt）， JUN， and HSP90AA1 and signaling pathways such as AMPK， nuclear factor-κB （NF-κB）， and Janus kinase/signal transducer and activator of transcription （JAK/STAT）. Molecular docking results showed that β-sitosterol and stigmasterol had high binding affinity with IL-6， TNF， JUN， and HSP90AA1. As for the animal experiment， compared with the normal group， model group had low SNCV of sciatic nerve （P<0.01）， disordered and loose myelinated nerve fibers with axonotmesis and demyelinization， low mRNA expression of AMPKα， AMPKβ， peroxisome proliferator-activated receptor γ coactivator-1α （PGC-1α）， Sirtuin 3 （SirT3）， mitochondrial transcription factor A （TFAM）， and low p-AMPK/AMPK ratio in sciatic nerve （P<0.05， P<0.01）. Compared with the model group， TBK of the three doses raised the SNCV （P<0.01）， restored nerve morphology and nerve compactness， and increased the mRNA expression of AMPKα， AMPKβ， PGC-1α， SirT3， and TFAM （P<0.05， P<0.01）. The ratio of p-AMPK/AMPK in the high-dose and medium-dose TBK groups was higher than that in the model group （P<0.01）， while the protein expression in the low-dose TBK group was insignificantly different from that in the model group. ConclusionTBK exerts therapeutic effect on DPN through multiple pathways and targets. The mechanism is that it activates and regulates AMPK/PGC-1α/SirT3 signaling， which lays a basis for further study of TBK in the treatment of DPN.
ABSTRACT
OBJECTIVE To systemically sort out the main practices of local drug online procurement， so as to provide reference for national and local governments to improve drug online procurement policies and drug price formation mechanism. METHODS By searching the official websites of 31 provincial medical security bureaus and drug-centralized procurement platforms in China， the classification methods and main goals of local drug online procurement were summarized， then the main practices of local drug online procurement were analyzed based on the main goals， and corresponding suggestion was put forward. RESULTS & CONCLUSIONS There were two classification methods of online drug procurement in each province， one is based on online or purchase methods， and the other is based on drug dimensions. Five main objectives of online drug procurement in each province are to ensure reasonable drug prices， meet clinical drug demand， ensure stable drug supply， cooperate with the implementation of drug policies， and standardize the behavior of transaction subjects. In order to achieve the five goals， each province has taken some targeted measures， but the severity of the policy varies greatly. In order to further improve the online drug procurement policy and improve the market-oriented online drug price formation mechanism， it is suggested to build the top-level design of online drug procurement， strengthen the management of the price comparison relationship of similar drugs， give full play to the monitoring function of the drug-centralized procurement platform and establish a national unified drug-centralized procurement platform.
ABSTRACT
Background Most metro system are underground, airtight, with inadequate ventilation and massive gatherings, posing health risks to metro riders. Objective To evaluate air quality of Metro Line 1 in a city, and provide suggestions and basis for preventing harmful factors and protecting the health of passengers. Methods Station halls, station platforms, and metro carriages of Metro Line 1 in a city were monitored in summer (from July to August in 2021) and winter (from January to February in 2022). Six metro stations were selected by stratified sampling. Each station and carriage were monitored for three consecutive days in rush hours (9:00–11:00 and 19:00–21:00) and non-rush hours (11:00–13:00), with the same monitoring frequency. The monitored indicators were physical factors (temperature, relative humidity, wind speed, illumination, and noise), chemical factors (carbon monoxide, carbon dioxide, inhalable particles, formaldehyde, benzene, toluene, xylene, ammonia, and ozone), biological factor (airborne total bacterial count), and radiation factor (radon). The monitoring results were compared by location, time period, and season. Results According to the Hygienic indicators and limits for public places (GB 37488—2019), the selected physical factors did not meet the standard, especially the temperature and relative humidity of station hall and platform, and wind speed and noise of carriage. The results of physical factors varied significantly by location (P<0.05). In summer, the temperature of carriage [M (P25, P75), 23.9 (23.3, 24.6)℃] was the lowest, and the wind speed [0.78 (0.37, 1.11) m·s−1] and noise [76.0 (72.0, 80.3) dB] of carriage were the highest; in winter, the temperatures of station hall and platform were the lowest [16.2 (13.2, 17.2)℃ and 16.2 (13.4, 17.0)℃, respectively], the relative humidity of carriage [26.4% (24.2%, 27.9%)] was the lowest, and the wind speed and noise of carriage were the highest [0.83 (0.47, 1.18) m·s−1 and 74.5 (70.1, 78.3) dB, respectively]. The physical factors varied significantly by time period (P<0.05). In summer, the temperature was the lowest during morning rush hours [24.0 (23.0, 24.8)℃] and non-rush hours [24.2 (23.2, 24.9)℃], and the relative humidity during evening rush hours was the lowest [41.9% (37.0%, 47.8%)]; in winter, the temperature was the lowest during morning and evening rush hours [16.8 (13.4, 19.7)℃ and 16.5 (15.1, 19.4)℃, respectively], the relative humidity during the non-rush period was the lowest [26.8% (24.7%, 28.6%)], and the wind speed during evening rush hours was the highest [0.28 (0.19, 0.51) m·s−1]. All measured chemical factors, biological factor, and radiation factor met the national standard (GB 37488—2019). Conclusion The chemical, biological, and radiative factors are complied with the national standard (GB 37488—2019) except physical factors such as temperature, relative humidity, wind speed, and noise. We suggest that the metro operators make full use of air conditioning system in combination with humidifiers to better regulate temperature and relative humidity, and .arrange working hours reasonably and provide noise-proof earplugs for carriage staff to protect against noise hazard.