ABSTRACT
This experiment aims to explore the metabolites of n-butanol and water soluble fraction of an ethanol extracts from Angelicae Sinensis Radix in rats. The chemical constituents of n-butanol and water extracts from Angelicae Sinensis Radix were identified by HPLC-DAD-ESI-IT-TOF-MS~n,and the in vivo metabolites of n-butanol and water extracts were analyzed. By analyzing n-butanol and water extracts from Angelicae Sinensis Radix,25 compounds were detected and identified,in which 11 phthalide glycosides were firstly reported. And 19 compounds were detected and identified in rat urine,including 2 prototype constituents and 17 metabolites,and the17 metabolites were new compounds. The method can identify the main constituents and metabolites of extracts from traditional Chinese medicine accurately and rapidly,and provide evidence for interpreting effective forms and pharmacodynamics substance( prototype,metabolites,or both) of Angelicae Sinensis Radix.
Subject(s)
Animals , Rats , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Glycosides , Medicine, Chinese Traditional , Spectrometry, Mass, Electrospray IonizationABSTRACT
Objective: To investigate the low polarity components in the stems of Uvaria macrophylla Roxb. var.microcarpa(Champ.)Finet et Gagnep.(Annonaceae). Methods: The dry stems of the title plant were extracted by re-fluxing with 95% ethylalcohol(80℃),and the obtained aqueous alcohol solution was evaporated to remove alcohol,andthen the ethyl alcohol extracts were extracted with petroleum ether(PE)to obtain a PE extract. The chemical constitu-ents in the PE extract were analyzed by GC-MS and identified by comparison of the obtained MS data with the standardmass spectral data. The relative contents in percentage were calculated for the constituents using the area normalizationmethod. Results: Ten compounds were identified,in which the palmitic acid(16.76%),4(14)- 11- eucalypene(15.31%),and selinene(14.45%)were the main components. Conclusion: The low polarity components in the stemsof Uvaria macrophylla Roxb. var. microcarpa(Champ.)Finet et Gagnep. mainly consisted of naphthalenes(31%),fol-lowed by sesquiterpenoids(29.76%).