Effect of epinephrine on the in vitro biosynthesis of fibrinogen.

In an in vitro rat liver slice incubation system, the synthesis of fibrinogen, when measured by immunoprecipitation technique was stimulated in the presence of epinephrine. An increase in poly (A)+ RNA content of the liver slice was also observed after epinephrine treatment. This in vitro experiment demonstrated that epinephrine stimulated de novo synthesis of fibrinogen by acting directly on the liver.

Characterization of foot-and-mouth disease virus types Ο and Asia 1 RNA.

Poly (A) RNA was isolated from foot-and-mouth disease virus-infected cells by oligo (dT)-cellulose chromatography. One-dimensional oligonucleotide mapping of virusinduced poly (A) RNA indicated major differences between virus types Ο and Asia 1. Base composition analysis of virus-induced RNA showed no significant differences between types O and Asia 1.

Preformed messengers in Microsporum canis macroconidia.

Macroconidia of Microsporum canis, when placed in a nutrient medium produce germ tubes within 4–6 h. Precursor incorporation studies showed that protein synthesis occurred prior to RNA synthesis. Sucrose density gradient analysis of wet and dry spore extracts revealed the presence of 16 % and 11 % polysomes respectively. The polysomal content increased to about 50% within 15 min of germination. Synthesis of RNA occurred only after 2 h of germination. Pool equilibration of the radioactive precursors was not limiting to these measurements. Polyadenylated RNA was isolated from macroconidia and was found to comprise 2-2·5 % of the total RNA. The poly(A)+ RNAs were heterodisperse and translatable in a wheat germ cell free translating system. It was concluded that macroconidia of Microsporum canis contain pre-formed mRNA which is translated early in germination.