ABSTRACT
ObjectTo study the effect of polydatin (PD) and asymmetric dimethylarginine (ADMA) on the endothelial function in aorta vascular strips of healthy rabbits and the interaction between PD and ADMA. MethodsDose-response curves of phenylephrine (PE) on the aortic strips with or without ADMA and/or PD. E max and Kd from PE were obtained and compared. ResultsNormal aortic strips could not respond to ADMA. Pretreatment with either PD or ADMA had no effect on the contractive response of aortic strips to PE. But PD could significantly weaken the contractive response of aortic strips pretreated with ADMA caused by PE in a dose-dependent manner, increase Kd and decrease E max of PE. ConclusionEither ADMA or PD alone does not influence the normal aortic strip contractive functions, the PE affinity to ?-receptor, and E max. But PD could noncompetitively antagonize the contractive reaction of aortic strips to PE in the presence of ADMA.
ABSTRACT
Objective:To evaluate the activity of nuclear factor kappaB(NF-?B)and the gene expression of high mobility group box 1(HMGB1)in lung injury induced by intestinal ischemia-reperfusion(IIR),and to investigate the protective effect of polydatin(PD)in this lung injury.Methods:Fifty-four male Sprague-Dawley rats weighing 200~250 g were randomly divided into control group,IIR group(45 min intestinal ischemia with 0.5 h or 2 h or 6 h reperfusion)and PD group [IIR with PD(10 mg/kg)treatment].The level of pulmonary myeloperoxidase(MPO)was detected by colorimetric method,the expressions of NF-?B and ICAM-1 by immuneochemistry and that of pulmonary HMGB1mRNA by RT-PCR respectively.Results:Lung injury induced by IIR was characterized by pathological damage of edema,hemorrhage and inflammatory cell infiltration.Compared with control group,pulmonary W/D and MPO increased significantly(P