ABSTRACT
Aims:The objective was to compare the sedimentation spontaneous in tube technique(SSTT), the direct smear, the zinc sulfhate(surface film and sediment analyzed) methods with the formalin-ethyl acetate method as the reference standard.Study Design:This a cross-sectional study performed in two populations aroundChapala lake, Jalisco, Mexico.Place and Duration of Study:Sample Department of Medical Sciences and Life, University Center of the Ciénega, University of Guadalajara, Ocotlán, Jalisco, Mexico. Department of Physiology, Health Sciences University Center. University of Guadalajara, Jalisco. México, between June 2018 andJuly 2019.Methodology:Sample: We included a total of 297 samples and were analyzed by the direct smear, the zinc sulfhate (surface film and sediment analyzed), the SSTT and formalin-ethyl acetate methods.Results:The SSTT was able to detect 40% of intestinal parasites, very good agreement for Entamoebacomplex, Entamoeba coli, andAscarislumbricoideskappa index=0.697, 0.791, 0.696, respectively and excellent agreement for Giardia lambliakappa index = 0.843. Regarding the isolation ofBlastocystisspp, only a poor agreement was found among all techniques. The SSTT was able to detect multiple parasites with a sensitivity of 82.90% and specificity 83.80 kappa index= 0.649.Conclusion:The SSTT showed a very good agreement for the diagnosis intestinal polyparasitism which could represent another alternative for the concentration and identification protozoans and intestinal helminthes in low-resource settings
ABSTRACT
Intestinal and urinary parasitic infections remain an important public health problem in the tropics where poor hygiene and sanitation are a norm rather than an exception. This study was designed to determine the prevalence and distribution of polyparasitic infections and to determine the impact of health education and treatments .Stool and urine samples from 350 participants were analyzed using macroscopic and microscopic direct stool examination of wet and Lugol’siodine preparations and Formal-ether concentration techniques and microscopic examination of simple centrifuged samples of urine. Out of the 350 participants, 101were infected with intestinal parasites with the male and female participants having 29 (66.0%), 41 (71.9%) prevalence of Entamoeba histolytica,9 (20.5%),8(14%) Eschericia coli,2 (4.6), 6(10.5%) Trichomonas hominisand4 (9.1%), 2 (3.5%) Chilomastix mesnillirespectively. Out of the infected 101 participants, 14 of them hadco-infection (polyparasitism). The result of polyparasitism in the Local Government is not significant (p>0.05).The overall infection before health education and treatment was high and least after health education and treatment 0(0%) (p<0.05). The results of this study indicated improper hygiene and sanitation conditions
ABSTRACT
Se realizó un estudio descriptivo y transversal de 320 pacientes mayores de 5 años de edad, cuyos exámenes de heces fecales dieron positivos, atendidos en el Centro de Diagnóstico Integral "Cuyuní" de la parroquia La Sabanita en Ciudad Bolívar (Estado Bolívar de Venezuela), desde julio del 2011 hasta marzo del 2012, a fin de caracterizarles según algunas variables de interés: tipos de parásitos, particularidades del abastecimiento y tratamiento del agua de consumo, lugar de deposición, hábitos higiénico-sanitarios y síntomas más frecuentes. Del total de la serie, 58,4 % presentaba poliparasitismo, 73,7 % se abastecía de agua mediante pipas o tanques y 45,3 % no trataba el agua de consumo, mientras que 66,3 % lo hacía por el método de filtración; asimismo, predominaron la defecación en letrinas (53,1 %), el hábito de andar descalzos (24,7 %), el dolor abdominal entre los síntomas referidos (39,4 %) y la Entamoeba histolytica como parásito más hallado (35,3 %).
A descriptive and cross-sectional study was carried out in 320 patients over 5 years of age whose stool tests were positive, treated in "Cuyuní" Comprehensive Diagnosis Center from La Sabanita parish in Bolívar city (Bolívar State of Venezuela), from July 2011 to March 2012, in order to characterize them according to some variables of interest: types of parasites, particularities of supply and treatment of drinking water, stools place, health habits and most frequent symptoms. Of the total of the series, 58.4% had polyparasitism, 73.7% were supplied with water by means of tankers or water tanks and 45.3% did not treat drinking water, while 66.3% did so by the filtration method; also, passage of stools in latrines (53.1%), the habit of walking barefoot (24.7%), abdominal pain between the symptoms reported (39.4%) and Entamoeba histolytica as the most found parasite (35.3%) prevailed.
Subject(s)
Parasites , Parasitic Diseases , Entamoeba histolytica , Drinking Water , Water , Bathroom Equipment , Child , Water Purification , Dysentery, AmebicABSTRACT
Disturbing data reveal the prevalence of intestinal parasites and their relationship with socio-environmental factors among Mbyá-Guarani Indians. The prevalence was determined by spontaneous sedimentation in water, centrifugation-floatation, and Kato-Katz. A socioeconomic questionnaire was submitted to each family. The overall prevalence of intestinal parasites was 88.7%, and 45.5% were polyparasitized. There was 90.5% prevalence of enteric parasites in children (1-12- year-old), and 85% among 13-65-year-old individuals, indicating that both age groups are extensively parasitized. The parasite load was low to moderate for geohelminths and 75% of the families did not have latrine, thus the practice of defecation occurred outdoors. These findings suggest that the multiple intestinal parasitism in the Mbyá-Guarani community is high to the point of being the rule, and that it relates essentially to the traditional lifestyle and health habits. It is urgently necessary to implement the association of anti-parasitic treatment with sanitation improvement. This should be done simultaneously with health education activities for this population.
Dados preocupantes demonstram a prevalência de parasitos intestinais e sua relação com fatores socioambientais entre indígenas Mbyá-Guarani. A prevalência foi determinada pelas técnicas de sedimentação espontânea em água, centrífugo-flutuação e Kato-Katz. Para cada família, um questionário socioeconômico foi aplicado. A prevalência geral de parasitos intestinais foi de 88,7% e 45,5% estavam poliparasitados. A prevalência em crianças (1-12 anos) foi 90,5% e em indivíduos entre 13-65 anos foi 85%, indicando que ambas as faixas etárias são amplamente parasitadas. A carga parasitária foi baixa a moderada para geohelmintos e 75% das famílias não possuem banheiro, sendo a prática da defecação feita ao ar livre. Estes achados sugerem que o poliparasitismo intestinal na comunidade Mbyá-Guarani é altíssimo, a ponto de ser a regra, e que se refere essencialmente ao estilo de vida tradicional e hábitos de saúde. Há a necessidade de aplicar urgentemente a associação de tratamento antiparasitário às melhorias sanitárias. Isto deve ser feito simultaneamente com atividades de educação em saúde para esta população.