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Objective To investigate the effects of polysaccharide sulfate(PSS)on lipopolysaccharide(LPS)-induced mice pulmonary microvascular endothelial cell(PMVEC) injury in vitro .Methods PMVEC were divided into the blank control group (C) ,LPS stimulation group (L) and PSS+ LPS group (LP) .The effect of PSS on the viability of LPS-induced PMVEC was ob-served by MTT assay .The influence of polymorphonuclear(PMN) on the PMVEC adhesion number was measured by the rose Ben-gal staining .The concentrations of TNF-αand ICAM-1 in culture supernatant of PMVEC were detected by ELISA assay .Results PSS could inhibit the decrease of PMVEC viability caused by LPS(P=0 .001) ,compared with the group C ,the adhesion number of PMVEC and PMN in the group L was significantly increased(P=0 .000) ,the expression of TNF-αand ICAM-1 was significantly increased(P=0 .000);compared with the group L ,PSS pretreatment for 1 h could significantly decrease the LPS caused adhesion of PMVEC and PMN(P=0 .000) ,the expression of TNF-αand ICAM in the group LP was significantly decreased (P<0 .05) .Con-clusion PSS can inhibit LPS-induced PMVEC injury and adhesion of PMVEC and PMN ,its mechanism may be related with the de-crease of ICAM-1 and TNF-αexpression .
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Polysaccharide sulfate (PSS) is a new type of antiatherosclerotic medicine for its effects of anticoagulation, anti-thrombosis and modulation of dyslipidemia. However, it is still uncertain whether PSS could modulate the diabetic dyslipidemia or not. Here, the rat model of diabetic dyslipidemia was developed and the effects of PSS on glucose and lipid levels were investigated in this animal model. Wistar rats were iv injected with streptozotocin 20 mg·kg-1 after feeding with high fat diet for one and a half month. Then, rats received orally PSS (30, 90, and 180 mg·kg-1) for 1 month. After oral treatment with PSS (90 and 180 mg·kg-1) for 1 month, the levels of triglyceride (TG), total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C) were significantly reduced and the level of high density lipoprotein-cholesterol (HDL-C) increased, compared with diabetic control rats. Moreover, PSS (30, 90, and 180 mg·kg-1) had a tendency to reduce glucose and insulin levels, and significantly increased insulin sensitivity index. Our results suggest that PSS could improve insulin sensitivity and relieve dyslipidemia in diabetic dyslipidemic rats.
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0.05),but the level of P- selectin in treatment group were obviously decreased compared with control group after the seventh and fourteenth day(P
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OBJECTIVE:To study the general pattern and characteristics of adverse drug reactions(ADR)induced by polysaccharide sulfate(PSS).METHODS:A retrospective study was carried out to collect and statistically analyze62cases of ADR induced by PSS reported in domestic academic journals between January1994and December2005.RESULTS:The ADR to PSS were independent of patient’s sex and dependent of patient’s age,administration routes and dosage.The ADR occurred at different intervals after drug application.Clinical manifestations of ADR were multiple involving several organs and systems,mainly damage of immune system and musculoskeletal system.CONCLUSION:Clinical doctors and pharmacists should under-stand the pattern and characteristics of ADR induced by PSS,and strengthen monitoring of its use in order to reduce occurrence of ADRs.
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32 Cases of coronary heart diseases (M 20, F 12; age 58+10 y) were treated with polysacchairde sulfate (PSS). PSS 200 mg in 10% glucose solution 500 ml iv, drip, qd, and 14 d, as a course. The results showed; The cardiac function, blood fat and indices of hemorheology were significantly improved ( P
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Objective To investigate the effects of polysaccharide sulfate(PSS)on the growth of vascular endothelial cell cultured in vitro and the adhesion of vascular endothelial cell to polymorphonuclear neutrophils(PMN).Methods ECV-304,a kind of human umbilical vein endothelial cells,was cultured in vitro with different concentrations of PSS(0,50,100,150,200,250 g?mL-1),the growth of ECV-304 was observed by means of MTT.Hypoxia/ reoxygenation(H/R) model of ECV-304 was established,the adhesion rate of PMN from patients with cerebral infarction to cultured ECV-304 was detected with or without treatment of PSS and H/R.Results The growth of ECV-304 in PSS-treated subgroups(final concentrations: 50,100,150,200 g?mL-1) were much better than in non-PSS subgroup,especially in subgroup with PSS final concentration of 100 g?mL-1.The adhesion rate of cultured ECV-304 to PMN from patients with cerebral infarction was higher significantly than that from the healthy subject,which was increased when ECV-304 being treated with H/R.The adhesion rate of cultured ECV-304(whether treated with H/R or not) to PMN was decreased significantly when ECV-304 being treated with PSS(100 g?mL-1).Conclusion The experiments in vitro showed that PSS could promote the growth of cultured ECV-304 and decrease the adhesion rate of H/R ECV-304 to PMN.
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Objective To observe the efficacy and safety of polysaccharide sulfate in treating patients with cold erythema multiforme.Methods Using polysaccharide sulfate,cetirizine and compound vitamin E ointment to treat 20 patients with cold erythema multiforme as the treatment group compared with the control group in which 20 patients with cold erythema multiforme were given cetirizine and compound vitamin E ointment.The course of treatment in 2 groups was 14 d.Results Forty patients were eligible for efficacy analysis(20 in each group).The total significant recovery rate was 95% for treatment group and 70% for control group,respectively;the therapeutic effects of the treatment group were better than those of the control group(P
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To comparative study the effect of polysaccharide sulfate (PSS) and amlodipine on renal hemodynamics in dogs. The kidney was denervated, PSS or amlodipine was injected through renal artery. Renal hemodynamic parameters, including mean arterial pressure, renal blood flow, glomerular filtration, urine flow, et al, were measured. Both PSS and amlodipine significantly increased renal blood flow and urine flow (P
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The changes of the hemorheology, plasma cholesterol and albumin and clinicaleffects in 36 children with refractory nephrosis after treatment with polysaccharide sulfate (PSS) were observed. The results showed that the indices of hemorheology and the plasma cholesterol decreased obviously and the albumin increased obviously than the control group ( P
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Thirty -nine cases of hypertension with blood hyperviscosity and deformed wave C dy/dt max,diagnosed by admittance cardiogram,were divided into two randomized groups,eighteen patients received PSS therepy while the other groups treated with Dan -Shen Co and Xin Nao Shu Tong. The results showed that hyperviscosity was controlled,and deformed wave C dy/dt max was recovered,and PSS group preferred to the control. The total effective rate for the former was 77. 8% and the latter 42. 9% ( P
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Objective To investigate the effects and mechanism of action of PSS on the treatment of patients with early DN. Methods 76 patients with early DN were given PSS 100mg(iv gtt, qd) for 14 days. ET-1, TXB_2, 6-Keto-PGF_(1a), blood viscosity TG, TC, HDL-C, LDL-C and urine protein quantity(24h UPQ) were determined and compared between before and after treatment. Results All indexes above were improved significantly after therapy. Conclusion PSS may modulate ET-1 secretion and the ratio of TXB_2/6-Keto-PGF_(1a), reduce blood lipid content and viscosity, improve hemorrheology in patients with early DN.
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Objective To investigate the effects of Polysaccharide Sulfate (PSS) on plasma von Willebrand factor (vWF) and thrombomodulin (TM) in patients with acute cerebral infarction.Methods 64 patients with acute cerebral infarction were randomly assigned to receive intravenous drip of PSS (150 mg/d for 14 days, 32 cases, PSS group) or Troxerutin (600 mg/d for 14 days, 32 cases, control group). All of the patients were tested for plasmatic levels of vWF and TM prior to and at 7 d, 14 d after treatment.Results The levels of vWF in PSS group at 7 d, 14 d after treatment were obviously decreased compared with control group (all P
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Objective:To investigate if a plant-derived polysaccharide sulfate(M33A) binding to HIV1 gp120 may induce the exposure of neutralization epitopes of gp120,and if M33A-bound HIV-1ⅢB antigens may be used as a AIDS vaccine for inducing neutralizing antibodies against HIV-1.Methods:Whole-inactivated M33A-bound HIV-1ⅢB antigens were prepared and used to immunize mice after mixing with FCA or FIA.The titers of anti-HIV-1 IgG antibodies in immunized mouse plasma were detected by ELISA,and the HIV-1 neutralization by those plasma was detected by the improved microtiter neutralization assay.Results:M33A-bound HIV-1 antigens induced higher titers of anti-HIV-1 IgG antibodies(group C:1.5?10~6;group D:1.5?10~6) than HIV-1 antigens alone(4.9?10~5),and female mice produced 3 times higher titers of anti-HIV-1 IgG antibodies than male mice after immunized with various HIV-1 antigens.All three immunization schemes did not induce the production of anti-HIV-1 neutralizing antibodies.Conclusion:M33A binding does not induce gp120 to expose neutralization epitopes.However,M33A may improve the level of mice immune responses to HIV-1 antigens,suggesting M33A may enhance immune response to HIV-1 antigens.