Effect of Administration of Chinese Traditiional Herbal Preparation for Invigorating Spleen and Strengthening Immunity on Immune Function and Exercise Capacity of Endurance Athletes / 中国运动医学杂志

Objective The effects of administration of Chinese traditional herbal preparation for invigorating spleen and strengthening immunity (ISSI) on immune function and exercise capacity of endurance athletes were observed. Methods Thirty-three elite cyclists and runners were randomly divided into two groups: ISSI group and control group. 200ml per dose of ISSI preparation were separately given to each athlete every morning and evening before meals for 1 month. Th 1/Th 2, NK cytotoxic activity, sIL-2R, IgG, IgA, IgM, serum testosterone (T) and cortisol (C), and VO 2max were measured. Results After 1 month of treatment, the immune function of ISSI group including NK cell activity, Th 1/Th 2 and IgG increased significantly (P

Evaluation of the transfection efficiency and cytotoxicity of polyamine cationic liposome / 解放军医学杂志

Objective To evaluate the transfection efficiency and cytotoxicity of polyamine cationic liposomes (PCL), and to screen for a cationic liposome with high potency and low toxicity. Methods Four kinds of PCLs, namely PCL-A, PCL-B, PCL-C and PCL-D, were prepared with polyamine cationic lipid TC-Chol or DC-Chol and the neutral phospholipid DOPE in different molar ratios (1∶1 or 3∶1). The morphology of the liposome was observed by transmission electron microscope. Plasmid PIRES2-EGFP, which contained an enhanced green fluorescencent-protein (EGFP) reporter gene, was transfected into Hela cells or Hep2 cells with PCL-A, PCL-B, PCL-C and PCL-D, respectively. Expression of EGFP was examined by fluorescence microscopy and the cytotoxicity of the transfected cells was determined with MTT assay. Results The shape of PCL-A, PCL-B, PCL-C and PCL-D was round or oval, most of the PCLs were about 50-200nm in diameter. The transfection efficiency of the four kinds of PCLs was about 19.8%-42.1%. Among them, PCL-C prepared with TC-Chol and DOPE at a molar ratio of 3∶1 showed a higher transfection efficiency (39.5%-42.1%) and lower cytotoxicity (cellular survival rate was 86.57%?3.23%-84.43%?4.18%). Conclusions The polyamine cationic liposome prepared with polyamine cationic lipids TC-Chol or DC-Chol and the neutral phospholipid DOPE at a certain molar ratio mediated the transfection of the plasmid PIRES2-EGFP into mammalian cells. PCL-C exhibited higher transfection efficiency and lower cytotoxicity, and it might be a promising agent for gene transfection and gene therapy.