Binding of Purine Nucleotides to Brown Adipose Tissue Uncoupling Protein from Different Kinds of Animals / 第二军医大学学报

Using brown adipose tissue submitochondrial particles from different kinds of animals, binding of uncoupling protein to different purine nucleotides was estimated. The results indicate that the dissociate constants (Kds) of uncoupling protein from hamsters to purine nucleotides are more than the Kds of uncoupling protein from guinea pigs, which are more than the Kds of that from rats, presumably because of different amino acid residue sequences of uncoupling protein polypeptide from different kinds of animals.