Biosynthesis of some organic acids and lipids in industrially important microorganisms is promoted by pyruvate carboxylases

Pyruvate carboxylase (Pyc) catalyzes formation of oxaloacetic acid from pyruvic acid by fixing one mole of CO2. Manyevidences have confirmed that biosynthesis of some different kinds of organic acids and intracellular and extracellular lipidsis driven by Pyc and over-expression of the PYC gene in the industrial microorganisms can promote production of thedifferent kinds of organic acids and intracellular and extracellular lipids. Therefore, the Pyc from different sources isregarded as a key enzyme in microbial biotechnology and is an important target for metabolic engineering of the industrialmicrobial strains. However, very little is known about the native Pycs and their functions and regulation in the industrialmicroorganisms

Effects of recombinded resistin on pyruvate carboxylase mRNA expression and activity in culture bovine hepatocyte in vitro / 中国兽医学报

A single factor duplicate test was designed to investigate whether bovine recombinded resistin impacts the expression of pyruvate carboxylase (PC) mRNA and the activity of PC in vitro culture bovine hepatocyte.Bovine recombinded resistin was added to the media with 0,25,50,100,200 and 400 ng/L.Abundance of PC mRNA in bovine hepatocyte,which was cultured with bovine recombinded resistin for 12 hours,was determined by real-time fluorescence quantitative RT-PCR,and activity of PC was determined by colourimetry.The results showed that bovine recombinded resistin could downregulate the expression of PC mRNA and the activity of PC in vitro culture bovine hepatocyte.

The Study of Enzymatic Activity of Mutant Strain Rhizopus oryzae with L-Malic Acid Accumulation / 微生物学通报

A stable and efficient L-Malic acid accumulation mutant strain Rhizopus oryzae ME-M15 was discovered occasionally in the mutation breading for fumaric acid producers. Rhizopus oryzae ME-M15 gave a L-Malic acid output of 16.3 g/L on average after fermentation for 96 hours, more than 3 times than that of the parent strain ME-F10. In addition, other metabolites such as ethanol and fumaric acid were re-markably decreased in accordance with the depressed activity of the cytosolic isoenzyme of fumarase and alcohol dehydrogenase in strain ME-M15, while the activity of the pyruvate carboxylase had no significant difference.