ABSTRACT
@#Objective To analyze rash and fever illness(RFI) and other respiratory virus co-infection in some confirmed measles cases in Jilin Province from 2013 to 2022,and to provide scientific basis for measles co-infection and multi-pathogen diagnosis.Methods The throat swab specimens of 106 measles confirmed cases in Jilin Province from 2013 to 2022 were collected,of which nine kinds of RFI associated virus and respiratory virus with similar clinical symptoms were detected by fluorescence quantitative PCR,including varicella-zoster virus(VZV),Dengue virus(DENY),human parvovirus B19(HPV-B19),Epstein-Barr virus(EBV),human herpesvirus6(HHV6),human rhinovirus(HRV),respiratory syncytial virus(RSV),human adenovirus(HAdV) and human cytomegalovirus(HCMV),and statistical analysis was conducted by using SPSS 23.0 software.Results VZV and DENV were not detected in the 106 collected specimens,and the other 7viruses were detected.30.18% of the measles cases were co-infected with other viruses,of which only HCMV co-infection cases showed significant difference in the age groups(≤24 months old,24 months old to 15 years old,> 15 years old)(χ~2=9.941,P <0.05);there was no significant difference between the genders in cases co-infected with other viruses(χ~2=0.200—2.778,each P> 0.05).Conclusion Some confirmed cases of measles might be co-infected with one or more other viruses in Jilin Province from 2013 to 2022,and it is recommended to strengthen targeted surveillance and differential diagnosis,especially for infants and young children,women of childbearing age and pregnant women.
ABSTRACT
Objective To investigate the pathogens spectrum and epidemiological characteristics of rash and fever illness (RFIs) from January 2010 to December 2017 in Pudong New Area, in order to provide scientific evidence for the prevention and control ofRFIs.Methods Enzyme-linked immunosorbent assay and real-time fluorescence quantitative polymerase chain reaction were used to detect the pathogens of enterovirus, measles virus, rubella virus and others from 2 831 clinical samples, and statistical analysis was performed.Results Pathogens were found in 1 633 samples in total, accounting for 68.59%. The top 4 viruses in the pathogen spectrum were enterovirus (52.54%), measles virus (28.54%), rubella virus (13.04%), and varicella-zoster virus (3.37%). There was significnat difference in the detection rate of rubella pathogens among patients of different genders(P=0.026). In the pathogen spectrum of infections of different age groups, the detection rate of enteroviruses at the age of 3-6 years was higher than that of other age groups. The detection rate of varicella-zoster virus at the age of 6-18 years old was higher than that of other age groups. The detection rate of virus including measles virus, rubella virus, dengue virus and small DNA virus in age of 18 and older was higher than that of other age groups. There was significant difference in the detection rate of pathogens in different age groups (all P<0.05).The incidence of RFIs was the highest in spring (41.52%) and the lowest in winter (15.00%). There was a statistical difference in the detection rate of enterovirus, measles, rubella and dengue virus in different seasons (P<0.05).Conclusions Enteroviruses and measles viruses are the main pathogens leading to RFIs in Pudong New Area, and the activity level of RFIs pathogens should be monitored for a long time.
ABSTRACT
Objective To develop a chemiluminescence imaging DNA microarray method for simultaneous,fast and accurate detection of nine rash-and fever-causing pathogens,namely,Measles virus,Rubella virus,Enterovirus type 71, Varicella zoster virus,Dengue fever virus,Human small FDNA virus B19,Coxsackie virus type A16,A-βStreptococcus pyogenes (hemolytic streptococcus)and Salmonella typhi.Methods Primers and probes were designed based on the specific sequence in the conserved region of genomes of the nine pathogens.The nucleic acids of the nine pathogens were amplified and labelled by multiplex PCR method.The multiplex PCR amplification products were hybridized with specific probes of microarray that was scanned after washing and chemiluminescence coloration to identify the nine pathogens.After the optimization of the multiplex PCR system,hybridization and chemiluminescence imaging,the specificity,sensitivity and reproducibility of the chip were evaluated.The serial diluted nucleic acid of Enterovirus type 71 was detected using microarray and real-time PCR approach to compare the sensitivity of these two methods.Results Nine specific primers and eleven specific probes were selected.The microarray demonstrated high sensitivity and specificity.The minimum detection limit of plasmid DNA and in vitro transcribed RNAs was 3 ×103 copies per reaction.The detection sensitivity of this microarray was 10 percent of that by the real-time PCR method.The rate of sensitivity and specificity of clinical sample detection was 95% and 85.7% respectively,and the rate of accuracy was 93.2%.Conclusion A chemiluminescence imaging DNA microarray method for simultaneous,fast and accurate detection of nine pathogens that cause rash and fever illnesses is established successfully,which can serve as a new high throuthput screening method for clinical diagnosis and epidemiological investigation of rash and fever illnesses.