ABSTRACT
Objective: To optimize the water extracting and refining procession of Sijunzi Decoction Granules (SDG). Methods: The orthogonal test method was used to study the four factors including the soak time, amount of water added, extraction time and frequency by taking the dry extract yield of the medicinal materials and contents of ginsenoside Rg1, Re, Rb1, glycyrrhizic acid, and liquiritin as indicators to optimize the water extracting process of SDG. To optimize the alcohol precipitation process of SDG, the factors including the concentration of the medicinal materials, alcohol content, and time of alcohol precipitation were investigated. The extracts before and after alcohol precipitation were compared by intervening spleen deficiency syndrome pharmacodynamics experiment. Results: The best water extracting procession of SDG was soaked for 60 min with 10 times of water, decocted three times, 30 min for each time. The optimal water extraction by alcohol sedimentation process was to concentrate the filtrate of water extraction to 1 mL, which was equivalent to 0.5 g of the original medicinal materials, with 80% alcohol content and 12 h alcohol precipitation time. Compared with the model group, the activity of salivary amylase and the conent of serum gastrin in the water extracting group and water extracting by alcohol sedimentation group of SDG were significantly increased and could improve the absorption function of xylose in small intestine of rats with spleen deficiency syndrome (P 0.05). Conclusion: The alcohol precipitation process is not suitable for refining of SDG. So the water extracting process is finally selected as the best extraction process of SDG.
ABSTRACT
Objective: To prepare modern tradional Chinese patent medicine Lemai capsules with a low dosage. Methods: Comparing the applications of seven kinds of macroporous adsorption resin in refinement of Lemai capsules' extracts in danshensu and paeoriflorin determined by HPLC as markers. Results: After refinement, the solid content of extracts decreased greatly. The content of danshensu became much lower than that before refinement The retention rate of AB 8、 X 5 and WLD for paeoriflorin is up to 80%. Conclusion: 7 kinds of macroporous adsorption resin are not proper to danshensu but some of them can be used to paeoriflorin.
ABSTRACT
Objective: To develop a stable and simple method to measure the content of aloe oligosaccharides. Methods: After the conversion coefficient of aloe oligosaccharides to glucose was obtained, the content of aloe oligosaccharides was measured by phenol sulfuric acid method. Results: The correlation coefficient of standard curve is 0.9993, the recovery rate is 95.3% and RSD is 1.23%. Conclusion: The method can be applied to aloe and aloe products.