ABSTRACT
In a double blinded study which was conducted at 3 kindergartens of 3 communes of Thanh Ba district and Phu Tho district showed that the effectiveness of Vi-rEPA for typhoid fever in 2-5 year old children reached to 89% for 46 months. Vi-rEPA containing 25mg of Vi induced more than 8 fold rise of IgG anti-Vi in all vaccines. The dosage immunogenicity study of 5, 12.5 and 25mg of Vi-rEPA was injected 6 weeks apart and blood samples were taken before, 10 weeks and 1 year after the first injection. At 10 weeks, the antibody levels of children with 25 mg injections were higher than that of children with 12.5mg and 5mg injection, and all 3 groups had antibody levels declined 7 folds, but still remained significantly higher than before immunization. This study confirmed the safety and immunogenicity of Vi-rEPA
Subject(s)
Child , Vaccines , Typhoid Fever , BloodABSTRACT
The nucleotide sequence of pZMO1, a small cryptic plasmid of Zymomonas mobilis ATCC10988 was determined. Analysis of 1,680 bp of sequence revealed 69% identity with Shigella sonnei plasmid, pKYM and 61% identity with Nostoc sp. ss DNA replicating plasmid. Analysis of a deduced amino acid sequence of an orf of pZMO1 revealed 75% identity and 90% similarity with the repA gene of Synechocystis sp. plasmid pCA2.4. The upstream region of the repA gene of pZMO1 possesses six directed repeat sequences and two inverted repeat sequences at downstream of the IR consensus sequence of nick region of rolling circle replication (RCR) plasmid. A typical terminator hairpin structure was found at the downstream region of repA gene. Degradation of single-stranded plasmid DNA by S1 nuclease was detected by Southern hybridization. It suggests that pZMO1 replicates by a rolling circle mechanism in Z. mobilis ATCC10988 cells.