Cytological study on vitreous extracts in Eales disease / 中华实验眼科杂志

To study the pathcytological mechanism of Eales disease and to provide a histological testimony for an effictive therapy.Methods45 cases(48 eyes) of Eales disease that were diagnosed according to vitreous hemorrhage and proliferative vitreoretinopathy or secondary retinal detachment.Cytomorphological examination was taken and vitreous extracts were examined by immunohistochemical staining.ResultLymphocytes and fibroblastes were most of those cells in the vitreous extracts.Immunohistology and electron microscopy revealed that lymphocytes were 78.8% or 61.5% in all specimens,respectively.ConclusionProliferative vitreoretinopathy induced by Eales disease closely relates with lymphocytes.The results suggested that autoimmunity might play an important role in the pathogenic mechanism of the disease

Comparative Effects of Retin-A and Vitamin C on Photoaging Skin

Photoaging skin occurs as a result of long-term exposure to ultraviolet radiation. In contrast to intrinsic aging, skin changes of photoaging can be reversed by the topical use of skin care products. Several skin care products have now undergone sufficient evaluation and have a well-defined role in our practice. Retin-A and alpha hydroxy acids have a significant number of data available for evaluation; data for Vitamin C and antioxidants are still emerging. We conducted clinical trial to compare the anti-photoaging effects of Rein-A and Vitamin C in 25 women volunteers. Each formulation wes applied daily to the randomly assigned hemifaces over the 8-month study period. Comparative evaluations of anti-photoaging effects were made using subject self-appraisal questionnares, plastic surgeon's assessment, ultraviolet revelations, and histologic examinations. Subject self-appraisal and plastic surgeon's assessment showed predominance of Retin-A over Vitamin C. But both Retin-A and Vitamin C provided objective and subjective improvement in photodamaged facial skin and no significant difference was found between Retin-A and Vitamin C in histologic examinations.

Damage of high glucose on retinal capillary pericytes / 眼科新进展

Objective　To observe the effect of high glucose on retinal capillary pericytes in vitro and research the pathogenesis of diabetic retinopathy (DR). Methods　Retinas dissected from eyes of fresh killed cattle were separated and retinal capillary pericytes were cultured. The effect of high glucose on pericytes was evaluated by measurement of lactate dehydrogenase(LDH),3H-thymidine incorporation and liquid scintillation techniques.Morphological changes were observed under microscope and electric microscope.Results　There was no significant difference in LDH activity in the supernatants between high glucose(10、20、40mmol*L-1) and normal glucose(5mmol*L-1)cultured for 48h or 96h. High glucose had an effective inhibition on pericytes' proliferation cultured for 48h,96h and 144h, and the inhibition rate increased when the glucose concentration rised and time passed by. The inhibition rate of pericytes was 78.09% after cultured in high glucose (40mmol*L-1) for 144h. Conclusion　High glucose has an effect inhibition on pericytes' proliferation, and the inhibition rate increase when the glucose concentration rises and time passes by.