ABSTRACT
Abstract@#Transport of filter paper-dried blood spot samples is a critical procedure during the screening of neonatal inherited metabolic diseases, which is of great significance for the screening accuracy. In order to ensure the timing and safety of sample transport, the cold chain positioning system was initiated by Zhejiang Provincial Center for Quality Control of Neonatal Disease Screening since March 2015. Based on the framework of neonatal disease screening information management system, the function of the logistics transport management system was included in this positioning system, with aims to achieve the monitoring and tracking of sample transport processes through real-time positioning of the sample transport box via China Unicom 4G logistics card and global positioning system/BeiDou Navigation Satellite System. The samples are maintained in a transport environment at 2 to 8 °C via the temperature-controlled box made of 5 °C phase-changed cold-stored materials and general packet radio service (GPRS) temperature recorders. The mean pretest turnover duration reduced from 8.44 days to 5.03 days following introduction of the cold chain positioning system, and the percentage of timely sample delivery increased from 31.69% to 77.90%, while the withdrawal rate of unqualified samples reduced from 0.12% to 0.08%. The cold chain positioning system meets the requirements of transport of filter paper-dried blood spot samples, which has a high potential in screening of neonatal inherited metabolic diseases.
ABSTRACT
O Whatman FTA-Card® é um papel-filtro quimicamente tratado, destinado à coleta, transporte, armazenamento de amostras para posterior extração de ácidos nucléicos. A tecnologia FTA-Card® é utilizada para manter estável DNA e RNA em temperatura ambiente, podendo ser utilizados para fixação de uma ampla variedade de material orgânico ou tecidos. Foram realizados testes para certificar sua eficiência na conservação do material a ser analisado com o intuito de eliminar a cadeia fria de conservação, agilizando o processo e diminuindo os custos da execução de exames moleculares associados ao diagnóstico de patologias. Foram testadas oito amostras de felinos na forma de sangue total e soro, para a extração utilizou-se o kit Magazorb RNA Total mini-prep kit (Promega®, EUA), para o diagnóstico foi utilizada a técnica de PCR em tempo real para amplificar o gene CI2 de mamíferos, a fim de visualizar a eficácia na conservação de ácidos nucleicos. A utilização desse método torna possível que o material biológico seja enviado por serviços de transporte postais, reduzindo os custos e viabilizando diagnósticos provenientes de áreas mais remotas.(AU)
Whatman FTA-Card® is a chemically treated filter paper intended for the collection, transport, and storage of samples for later extraction of nucleic acids. FTA-Card® technology is used to keep DNA and RNA stable at room temperature and can be used to fix a wide variety of organic material or tissues. Tests were carried out to certify its efficiency in the conservation of the material to be analyzed in order to eliminate the cold conservation chain, speeding up the process and decreasing the costs of performing molecular tests associated with the diagnosis of pathologies. By using this method, biological material can be sent by postal transport services, reducing costs and making diagnoses from more remote areas feasible. Samples of feline specimens were tested in the form of whole blood and serum, using the Magazorb RNA Total mini-prep kit (Promega®, USA) for the extraction. Diagnosis was performed using real-time PCR technique to amplify the mammalian CI2 gene in order to visualize the effectiveness in conserving nucleic acids.(AU)
Whatman FTA-Card® es un papel de filtro tratado químicamente, destinado a la recogida, transporte, almacenamiento de muestras para su posterior extracción de ácidos nucleicos. La tecnología FTA-Card® se usa para mantener el ADN y el ARN estables a la temperatura ambiente y se puede usar para la fijación de una amplia variedad de materiales o tejidos orgánicos. Se realizaron pruebas para certificar su eficiencia en la conservación del material a analizar con el fin de eliminar la cadena de frío de conservación, agilizando el proceso y reduciendo los costos de realización de pruebas moleculares asociadas al diagnóstico de patologías. Se analizaron ocho muestras felinas en forma de sangre total y suero, para la extracción se utilizó el mini-prep kit Magazorb RNA Total (Promega®, USA), para el diagnóstico se utilizó la técnica de PCR en tiempo real para amplificar el CI2 de mamífero gen, con el fin de visualizar la efectividad en la conservación de ácidos nucleicos. El uso de ese método permite el envío de material biológico por los servicios de transporte postal, lo que reduce los costes y permite realizar diagnósticos desde zonas más remotas.(AU)
Subject(s)
Biocompatible Materials , DNA , Real-Time Polymerase Chain ReactionABSTRACT
El procedimiento pre-analítico en el laboratorio clínico es un conjunto de pasos que inician a partir de la orden de examen realizada por el médico, verificación de la identificación del paciente, criterios de aceptación y rechazo de la muestra, recolección de la muestra, identificación de la muestra, transporte seguro, en temperatura y tiempo apropiado. Luego ingresan para su preparación, y termina en el momento que se inicie con el análisis de las pruebas. Objetivos: Determinar el cumplimiento del procedimiento pre-analítico que contribuye a la seguridad del paciente en el laboratorio clínico. Método: El presente, es un estudio observacional, de tipo cuantitativo, de alcance descriptivo no experimental, de corte transversal. Resultados: de acuerdo a los resultados obtenidos de 112 muestras, con base a la ficha de observación, se logró evidenciar que ha habido muestras con calidad inadecuada como son las hemolizadas, coagulas y contaminadas. Y por estar en esas condiciones, son rechazadas por el laboratorio clínico, lo que conlleva a que se realice otra toma en el paciente, causando malestar, demora en la entrega de sus resultados e insatisfacción. También se evidencia que las muestras hemolizadas son mayores a las coaguladas y contaminadas. Conclusión: Este estudio ha ayudado a conocer que en los puestos de toma de muestra no se está implementando las normas de calidad para evitar errores que han estado causando malestar en los pacientes. Así como también, los puestos de toma de muestra no disponen de equipos para ayudar a conservar las muestras y trazabilidad de sus análisis(AU)
The pre-analytical procedure in the clinical laboratory is a set of steps that start from the order of examination carried out by the doctor, verification of the patient's identification, criteria for acceptance and rejection of the sample, collection of the sample, identification of the sample, safe transport, at appropriate temperature and time. Then they enter for their preparation, and it ends when the analysis of the tests begins. Objectives: To determine compliance with the pre-analytical procedure that contributes to patient safety in the clinical laboratory. Method: This is an observational, quantitative, descriptive, non-experimental, cross-sectional study. Results: according to the results obtained from 112 samples, based on the observation file, it was possible to show that there have been samples with inadequate quality such as hemolizada, coagulated and contaminated ones. In addition, because they are in these conditions, the clinical laboratory the clinical laboratory rejected them, which leads to another taking in the patient, causing discomfort, delay in the delivery of results and dissatisfaction. It is also evident that hemolizada samples are greater than those that are coagulated and contaminated. Conclusion: This study has helped to know that in the sampling stations not implemented quality standards to avoid errors that have been causing discomfort in patients. As well as, the sampling stations do not have equipment to help conserve the samples their traceability and analyzes(AU)