ABSTRACT
Objective To research the chemical constituents in Comastoma traillianum. Methods The chemical constituents from C. traillianum were isolated and purified by column chromatographic methods of silica gel, RP-HPLC, Sephadex LH-20, and RP-C18. Their structures were elucidated by modern spectroscopy methods, including 1D and 2D NMR, HRESIMS, IR, and UV techniques. The anti-inflammatory activity of compounds against LPS and IFN-γ-induced mouse RAW 264.7 macrophages was determined by Griess, and the IC50 value was calculated to evaluate their anti-inflammatory activity. Results Four secoiridoid glycosides (1-4) were isolated from C. traillianum, and identified as comtraide A (1), deacetylcentapicrin (2), swertiamarin (3), and gentiopieroside (4). Compound 1 is a new compound named comtraide A. All compounds were tested for their in vitro anti-inflammatory activity and no activity was found. Conclusion Compounds 1-4 are isolated for the first time from C. traillianum, and compound 1 is a new compound. However, no significant anti-inflammatory activities were detected in all these compounds at concentrations up to 100 μmol/L.
ABSTRACT
Objective: To study the chemical constituents of Swertia cincta. Methods: Preparative liquid chromatography was employed. The structures of the compounds were elucidated by spectroscopic analysis. Results: Three secoiridoid glycosides were isolated from S. cincta and identified as 8-methoxyl-eustomorusside (1), secoiridoids eustomorusside (2), and eustomoside (3). Conclusion: Compound 1 is a new secoiridoid glycoside. Compounds 2 and 3 are isolated from this plant for the first time. © 2014 Tianjin Press of Chinese Herbal Medicines.
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Objective: To study the chemical constituents in the roots of Picrorhiza scrophulariiflora. Methods: The chemical constituents in the roots of P. scrophulariiflora were separated and purified with chromatographic methods. The structures were elucidated by spectroscopic methods and chemical analyses. Results: A new secoiridoid glycoside, picrogentioside II (1) was successfully isolated from the roots of P. scrophulariiflora. Conclusion: Compound 1 is a new secoiridoid glycoside. © 2013 Tianjin Press of Chinese Herbal Medicines.
ABSTRACT
Objective: To study the constituents in the roots of Picrorhiza scrophulariiflora. Methods: The constituents of P. scrophulariiflora were separated and purified with chromatographic methods. The structures were elucidated by spectroscopic methods and chemical analyses. Results: Four compounds isolated from the 90% ethanol extract in the roots of P. scrophulariiflora were identified as picrogentioside D (1), sweroside (2), gentiopicroside (3), and mannitol (4). Conclusion: Compounds 1-4 are obtained from the roots of P. scrophulariiflora for the first time and compound 1 is a new secoiridoid glycoside, named picrogentioside D.
ABSTRACT
Object To study the glycosides from Swertia erythrosticta Maxim Methods The glycosides were isolated on silica gel column and purified by Sephadex LH20, their structures were identified by spectral data and chemical properties Results Seven compounds were obtained from aqueous extract and identified as swertianolin (Ⅰ), norswertianolin (Ⅱ), norswertiaglucoside (Ⅲ), isoorientin (Ⅳ), loganic acid (Ⅴ), gentiopicroside (Ⅵ) and ? gentiobiose (Ⅶ) Conclusion Compounds Ⅲ, Ⅳ, Ⅴ, and Ⅶ were first obtained from this plant