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Objective:To study the factors of nickel titanium instrument(NTI)seperation in posterior tooth root canal therapy.Meth-ods:NTIs were used in 1 498 patients with 1 562 involved posterio teeth and 3 518 root canals for RCT from Jun.2009 to May.2012. The relation of instrument seperation(IS)with gender,age,tooth position,working years of the dentists,bending degree of the in-volved tooth canal and the NTI form was analyzed statistically.Results:68 out off 1 198 applied NTIs(5.68%)were seperated in cal-nal preparation.Single factor analysis showed that IS incidence was not statistically different between male and femal(χ2 =0.013,P >0.05),it was statistically different among the patient ages(χ2 =-354.258,P <0.05),between molar and premolar(χ2 =-201.344,P <0.05),among working years of the dentists(χ2 =13.763,P <0.05),bending degree of involved teeth(χ2 =22.414,P <0.05),times of NTI uses(χ2 =16.807,P <0.05),taper of NTIs(χ2 =18.283,P <0.05)and diameter of NTIs(χ2 =21.312,P <0.05).Logistic analysis showed that age,tooth position,working years of dentists,bending degree of involved tooth canal,frequency of NTIs applica-tion and NTI form were independent risk factors for NTI seperation.Conclusion:Dentists should take appropriate technique and stand-ard operation sequence to reduce the incidence of nickel titanium instrument seperation.
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[Objective]To observe the clinical results of a hollow compression nail applied to treat the separation of elements of neural arch.[Method]Since February,2003,sixteen cases of separation of elements of neural arch have been treated with a hollow compression nail and bone grafting .The mean age of the group was 32,from 18 to 54.Five cases of separation of elements of neural arch located at L4 and the other 11 at L5.All the patients suffered from severe back pain.During the operation,the presence of the pars interarticularis defect was confirmed.The defects were cleared of any fibrous and cartilaginous tissues.The sclerotic bone margins were curetted until bleeding was noted .Autograft harvested from posterior ilium was done.The hollow compression nail was placed onto the index vertebra.[Result]All the patients were followed-up for more than 14 months (14～26 months).The significant back pain relief was found in all the cases.The bony healing was comfirmed in 11 cases,and the other 5 were in the process of bone growth.[Conclusion]The separation of elements of neural arch can be treated by the hollow compression nail without spinal fusion.The hollow compression nail is proved to be safe and effective.
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OBJECTIVE:To establish a method and a technical process for the purification of total alkaloids from Gelsemium elegans Benth.with macroporous resin.METHODS:Different types of macroporous resin were used to separate and purify total alkaloids,the HPLC peak areas of the main ingredient of the alkaloids form Gelsemium elegans Benth.were compared and the optimum technological parameters were investigated.RESULTS:The HPD-500,HPD-600,HPD-800type macroporous resin showed better comprehensive absorption property,and HPD-800type macroporous resin were used to investigate parameter.CONCLUSIONS:The method of using HPD-800type and eluting with grade ethanol-water can be used to purify the total alkaloids from Gelsemium elegans Benth..
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Objective:To establish a method for isolation,purification,and culture of human multipotent adult progenitor cells(MAPCs) in vitro,so as to lay a foundation for the application of hMAPCs in tissue-engineering research and clinical medicine.Methods: The mononuclear cells were obtained from bone marrow of healthy volunteers by gradient centrifugation and were subjected to adherence culture.The adherent cells were then subjected to magnetic activated cell sorting(MACS)(depletion selection with CD45 and GlyA microbeads).Then the purity of selected cells was identified by flow cytometer.The growth of the purified cells was observed and the expression of CD29,CD44,CD34,and HLA-DR was analyzed by flow cytometers.Results: (1)Averagely(5-10)?10~(4)/ml hMAPCs could be separated from 1?10~(6)/ml bone marrow mononuclear cells by MACS.The cell viability was similar before(96.7?1.7%) and after(96.0?2.4%) isolation.(2)The isolated MAPCs grew well in the self-designed culture medium and could be passaged for 20 generation.(3)The purity of the CD45~-and GlyA~-cells separated from bone marrow adherent cells was more than 98% as determined by flow cytometer.(4) In hMAPCs,the positive rate was 99.2% for CD29,98.3% for CD44,1.2% for CD34,and 5.3% for HLA-DR.Conclusion:(1)The bone marrow-derived hMAPCs can be purified by MACS through depletion selection of CD45 and GlyA microbeads.(2)The hMAPCs can be expanded in vitro in self-designed medium,maintaining a non-differentiation state for a long time.
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Preparation of theophylline microcapsule by the coacervation - phase seperation method. Theophylline microcapsule has been prepared by coacervation - phase seperation, using ethyl cellulose as the coating material. Ethyl cellulose sustanined significally the release of theophylline from microcapsule. Microcapsule has been used for preparation of theophylline sustained - release tablet.
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Theophylline , CapsulesABSTRACT
Seperation anxiety disorder(SAD) is characterized by excessive anxiety about being apart from the individuals to whom a child is most attached. We noticed a case of a 13 year old boy's SAD with symptoms of nausea/vomiting, stomachache, school refusal, and severe weight loss. His mother was overanxious and overdependent but her'dependency was frustrated due to family problem. We investigated this case from a point of developmental aspect and family dynamics and tried to attempt therapeutic approach.
Subject(s)
Child , Humans , Anxiety Disorders , Anxiety , Disulfiram , Mothers , Weight LossABSTRACT
Bursa of Fabricius is a very important organ for B-eell development. We sot bursa extracts from 2-3 months old Peking Duck. A partially purified PFC-stimulating active factor has been seperated from the extracts by twice molecular sieve chromatography, Sephadex G-15 and Moselect G-10. The M. W. of the active factor is below 750 dal. The results of HPLC analysis of the factor show that its polarity is relatively strong, and it has more positive charges.The works of purifying and looking for a new assay are still going on.
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There are many procedures described for the treatment of acromioclavicular seperation but there are still controversies concerning the best management of these injuries. Thirteen cases were operated on by technique of modified Phemister method and four cases by Bosworth method, Dept. of Orthopedic Surgery, Chonbuk National University Hospital from January 1980 to December 1984. The following results were obtained. The following results were obtained. 1. This injuries is more prevalent in male (76.4%) with peak incidence in the second, third and fourth decades(76.4%). 2. The most common causes of the injuries were traffic accidents and followed by falling from the height. 3. Fifteen patients were grade 3 by Allmans classification. 4. Operative method consists of modified Phemister method (76.4%) and Bosworth method (23.6%). 5. The operative procedures in Type 2 and Type 3 are good treatment of acromioclavicular seperation.
Subject(s)
Humans , Male , Accidental Falls , Accidents, Traffic , Classification , Incidence , Joints , Methods , Orthopedics , Surgical Procedures, OperativeABSTRACT
Objective To isolate and culture the hair follicle Bulge cells of rats and study their morphological, immunological characteristics. Methods Hair follicle Bulge obtained by micromanipulation and enzyme digestion was cultured in intro. The morphological features and numbers of Bulge cells were identified and counted with light microscopy. Immunocytochemical staining was used to detect expressing of K19 and ?_ 1integrin in cultured cells. Results Bulge cells after enzyme digestion pasted rapidly. Cells were small and round, paving stone-shape, and had a large ratio of nuclear to cytoplasm. The characteristics suggested a primitive morphologic feature. Cells growth well and could maintain low differentiation and higher reproductive activity, they co-expressing K19 and ?_ 1integrin, immunofluorescence staining showed the cells expressed ?6-integrin strongly, weakly or no expressing CD71. Conclusion This culture system can amplify a lot of pure hair follicle Bulge cells in short time. We successfully isolate and culture the hair follicle Bulge cells of rats in intro and can keeping its property for a long time.