ABSTRACT
[Objective]To explore the change of serum gastrin and G-cell in gastric antrum in rat model of spleen-qi deficiency and the curative effect of huangqi jianzhong decoction(HQJZD).[Methods]Spleen-qi deficiency syndrome was induced in rats by compound methods.After the models had been established,they were treated by different dosages of HQJZD.And at different time points,serum xylose,creatine phosphokinase,gastrin and G cells were observed.[Results]These indexes in groups of different dosages of HQJZD recovered gradually.Especially after 21 days' treatment,in groups of the middle and the high dosages,these indexes were increasing significantly when compared with those in the model control group(P
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Objective: To explore the effect of buzhong yiqi decoction(BZYQD) with various concentrations at different time points on gastrin(GAS) gene expression level in spleen-qi deficiency rats.Methods: The spleen-qi deficiency rats were randomly divided into the model control group and the low,medium and high dosage of BZYQD groups.Reverse transcriptase polymerase chain reaction was used to observe the effect of BZYQD on the GAS mRNA of gastric antrum in rats treated at different time points(7,14,21d).Results: As compared with that in model control group,the GAS gene expression level were significantly higher both in the medium and high dosage groups(P
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Objective: To explore the effect of Buzhong Yiqi Decoction(BZYQD) with various concentrations at different time points on serum gastrin in spleen-qi deficiency rats.Methods: Spleen-qi deficiency syndrome was induced in rats by compound methods which including rhubarb method,swimming exhausting and hunger method.Spleen-qi deficiency rats were randomly divided into the model control group and the low,medium and high dosage of BZYQD groups,and Decoction of Four Mild Drugs(DFMD) group.Serum gastrin in different groups was observed at different time points(7d,14d,and 21d).Results: Compared with model control group, the serum gastrin in the medium and high dosage of BZYQD groups were both significantly higher(P
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Objective: To make comparative study on serum gastrin(SG) and gastrin secretory cell(G-cell) in gastric antrum(GA) of three rat models of syndrome of deficiency of spleen-QI.Methods: Animals were randomly divided into four groups in terms of control group,rhubarb group,double factors group(combination of rhubarb method and swimming exhausting energy method) and triple factors group(combination of rhubarb method,swimming exhausting energy method and starvation method).The indexes such as Serum gastrin and G-cell in gastric antrum(GA) et al were detected.Results: Compared with control group,the variances of above-mentioned indexes in double factors group and triple factors group have statistical significance(P
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Objective: To explore the effect of Sijunzi decoction(SJZD) at different time points on gastrin(GAS) gene expression level in spleen-qi deficiency rats.Methods: Spleen-qi deficiency syndrome was induced in rats by compound methods which including rhubarb,exhaust and hunger.Spleen-qi deficiency rats were randomly divided into the model control group and SJZD group.Reverse transcriptase polymerase chain reaction was applied to observe the GAS mRNA of gastric antrum in rats treated by SJZD.Results: Serum gastrin and GAS mRNA were both decreasing significantly in the model control group.After being treated by SJZD,serum gastrin and GAS mRNA of spleen-qi deficiency rats began to recover.Especially,after 21 days' treatment,GAS mRNA and serum gastrin were both increasing significantly when compared with those in the model control group.Conclusion: The regulation on GAS mRNA is one of the mechanisms of SJZD for spleen-qi deficiency.
ABSTRACT
Objective: To explore the effect of modified HuangqiJianzhong decoction(MHQJZD) with various concentrations at different time points on gastrin(GAS) gene expression level in spleen-qi deficiency rats.Methods: Spleen-qi deficiency syndrome was induced in rats by compound methods which including Radix et Rhizoma Rhei, exhausting and hunger.Spleen-qi deficiency rats were randomly divided into the model control group and the low,medium and high dosage of MHQJZD group.Reverse transcriptase polymerase chain reaction was used to observe the effect of MHQJZD on the GAS mRNA of gastric antrum in rats treated at different time points(7,14,21d).Results: As compared with that in model control group,the GAS gene expression level were both significantly higher in the medium and high dosage groups(P