ABSTRACT
Objective:To probe into Rab25 Gene’s Effect on TGF-β inhibition of proliferation, invasion and epithelial mesenchymal transformation (EMT) of breast cancer MDA-MB-231 cells and explore its molecular mechanism.Methods:The experiment was divided into three groups: control group,TGF-β Group and si-Rab25 group. TGF-β induced MDA-MB-231 cell model of EMT was built. CCK-8 assay was used to detect cell proliferation. Transwell assay was used to detect the ability of cell invasion and migration.Western blot was used to detect the changes of related proteins in each group.Results:After stimulating MDA-MB-231 cells with TGF-β, Rab25 gene was highly expressed. Compared with TGF-β group (57.48±%3.62%), the migration ability and invasion ability of cells in si-Rab25 group (33.49%±2.93%) decreased by 41.7%, with a significant difference ( P<0.05). Compared with TGF-β group (153.21%±4.17%), the proliferation ability of cells in si-Rab25 group (115.32%±5.69%) decreased by 24.73%, with a significant difference ( P<0.05). The expression of MDA-MB-231 fine EMT related protein in si-Rab25 group was significantly different from that in TGF-β group ( P<0.05). The expression of p-AKT and Snail protein in si-Rab25 group was significantly lower than that in TGF-β group ( P<0.05) . Conclusions:Rab25 gene is highly expressed in MDA-MB-231 cells. Silencing Rab25 gene can activate AKT signal pathway, inhibit Snail protein expression, regulate EMT related protein expression, and inhibit EMT transformation.
ABSTRACT
Objective To observe the effect of crocin pretreatment on the learning memory ability, the ultrastructure and SIRT1/PGC-1α expression in hippocampus under hypoxia at high altitude in rats. Methods 96 male SD rats were randomly divided into Hypoxia model group and Hypoxia treatment group with 48 in each.The group rats in treatment group were injected with Crocin once daily(50 mg/kg) and the model group were injected with the same dose of 0.9%NaCl for 3 days,and then they were shifted to high al-titude under hypoxic environment and then the samples were taken at 1 d,3 d,5 d,7 d.Morris water maze test was used to observe learning and memory behaviors of rats.The level of SIRT1/PGC-1αprotein was detected by Western blot and the level of mRNA was detected by RT-PCR.Results Compared with the model group (1d(37.55±1.34)s,3d(46.51±6.19)s,5d(42.12±2.73)s),treatment group showed significant less time in looking for platform in Morris water maze experiment at (1d(33.58±2.23)s,3d(30.19±3.35)s and 5d (37.10±1.48)s;all P0.05). Western blot showed that the protein expression levels of SIRT1 and PGC-1α in hippocampus of rats were higher in treatment group (1d(0.72±0.02),(0.72±0.02);3d(0.76±0.03),(0.75±0.021);5d(0.54± 0.03),(0.74±0.01)) than those in control group (1d(0.53±0.04),(0.62±0.04);3d(0.15±0.02),(0.13± 0.02);5d(0.23±0.03),(0.21±0.02);all P0.05).Conclusion Crocin pretreatment can ameliorate mitochondrial damage and improve the a-bility of learning and memory under hypoxia at high altitude in rats,which may be achieved through increased expression of SIRT1 and PGC-1α in the hippocampus.
ABSTRACT
Liver fibrosis occurs as compensatory responses to tis-sue repairing process in a wide range of chronic liver injures.It is characterized by excessive deposition of extracellular matrix (ECM)in liver tissues.The new discovery shows that suppres-sor of cytokine signaling (SOCS-3)is strictly associated with fi-brogenic progression of chronic liver diseases.Recent basic and clinical investigations also demonstrate that liver fibrogenesis is accompanied by SOCS-3,which critically determines the patho- genesis and prognosis of liver fibrosis.This review summarizes current knowledge on SOCS-3 and the relationships between SOCS-3 and liver fibrosis.On the other hand,it also presents the different strategies that have been used in experimental mod-els to counteract excessive SOCS-3 and the role of SOCS-3 in the prevention and treatment of liver fibrosis.