DNA extraction from human bone: comparison of magnetic bead and silica column techniques / Extração de DNA de ossos humanos: comparação entre as técnicas de partículas magnéticas e coluna de sílica

Objective: The present study aimed the comparison between the automated magnetic bead and silica column techniques for DNA extraction of human bones. Material and methods: Both techniques were performed on 25 human femur bones evaluating: I) the amount of extracted genetic material; II) the amount of amplified profiles; and III) the necessary time range to perform the techniques. Results: The automated magnetic bead technique recovered larger amount of DNA in 88% of the studied bone sample in comparison with the silica column technique. The automated magnetic bead technique also achieved a high level of amplifications (9/16loci) in 68% of the sample, while the silica column technique reached equal level of amplifications only in 36% of the sample. The time range elapsed for performing the automated magnetic bead technique was approximately 3 hours for processing 12 samples, while the silica column technique performed the same samples in 81 hours. Conclusion: Based on that, the automated magnetic bead technique presented optimal outcomes and faster performance compared to the silica column technique, revealing a valuable tool for forensic DNA extraction.

Objetivo: O presente objetiva comparar as técnicas de partículasmagnéticas e de coluna de sílica para a extração de DNA a partirde ossos humanos. Materiais e métodos: Ambas as técnicas foramaplicadas em 25 fêmures humanos, visando avaliar: I - a quantidadede material genético extraído; II - a quantidade de material amplificado;e III - o tempo decorrido para a aplicação de cada técnica.Resultados: A técnica de partículas magnéticas viabilizou maiorquantidade de material extraído em 88% da amostra. A mesmatécnica alcançou também maior quantia de material amplificado(9/16 loci) em 68% da amostra. O tempo decorrido para a aplicaçãoda técnica de partículas magnéticas consistiu num período de 3horas para o processamento de 12 amostras, enquanto a técnica decoluna de sílica realizou o mesmo procedimento em 81 horas. Conclusão:Desta forma, a técnica de partículas magnéticas apresentouresultados mais satisfatórios dentro de um desempenho mais rápidoquando comparada com a técnica de coluna de sílica, revelandoser uma eficaz ferramenta forense.

Isolation and identification of curdione in zedoary oil / 中成药

AIM:To study the isolation and identification of curdione from Zedoary oil. METHODS: Silica column was adopted to isolate constituents from Zedoary oil;IR,GC/MS and NMR methods were used to identify its structure. RESULTS: Three constituents were isolated from Zedoary oil,including crystal C_1,C_2 and C_3.Crystal C_1 was identified to be curdione and curdione content in Zedoary oil was 11.31%. CONCLUSION: This method is simple and convenient and it can be used to isolate more quantity of curdione for further pharmaceutical study.