Platelet Activation of Stored Platelets with Storage / 대한수혈학회지

BACKGROUND: Effects of storage period on platelet activation of random-donor platelets (RDP) prepared from whole blood units and single-donor platelets (SDP) prepared from single-donor apheresis collections have been investigated in this study. We also analyzed the correlation between amount of blood cells and platelet activation in random-donor platelets. METHODS: RDP and SDP were collected at 1 day, 3 day, or 5 day during storage. In case of SDP, whole blood was also collected just before apheresis. The platelet activation in RDP and SDP was measured by flowcytometry using monoclonal antibodies against CD41a, CD61 and CD62p. RESULTS: In SDP, MCFI against CD62p has been significantly increased during storage and any significant differences are not found according to the kinds of pheresis machines. In RDP, no significant differences in MCFI against CD62p were found with storage period and showed a increased MCFI dependent only on the number of platelets. CONCLUSION: Single-donor platelets should be used as soon as possible for transfusion due to progressive platelet activation with storage period. On the other hand, a proper number of platelets should be maintained under strict quality control system to minimize platelet activation in RDP.

A View of Supply of Single Donor Platelets in Korean Red Cross / 대한수혈학회지

BACKGROUND: The use of single donor platelets (SDPs) has been increased substantially worldwide. But in korea, SDPs are supplied only 11.5% of total platelets transfused and most of them collected in hospital blood center. Korean Red Cross blood center decided to supply and started collection of SDPs from registered and random repeat donors since January, 2000. METHODS: The utilization of platelets including SDPs was analyzed by mail survey from 33 hospitals and by statistical yearbooks of KRC, 2000. The necessity of SDPs and supplementary amounts of SDP in case of need was estimated. RESULTS: Total 483,611 units of platelets (692-80,414 units) and 12,150 units of SDP(0-3364 units) were utilized by 33 hospitals participated mail survey. The mean discard rate of platelets in the 33 hospitals was 1.5% (0%-15%). The total amounts of SDP collection in 19 hospitals among them were 10,031 units. About one third of 29 hospitals investigated, call for to use 100% of SDPs for patients. And most of the hospitals were cooperative using SDPs supplied from KRC. The amounts of SDP used in Big 5 hospitals, which is located in Seoul was 76.1% of total SDPs used in Korea. CONCLUSION: The relevant amount of supplied SDPs would be 80.000 unit/year, which is 50% of total supplied platelets by KRC. Efficient utilization of SDPs needs well organized collection, SDP stock control and supply system by regional groups of KRC blood center. In additionally, donor recruitment and maintenance for substantial supply of SDPs, and the supplementary support from the government is important.

Affecting Factors on the Willingness to Platelet Apheresis / 대한수혈학회지

BACKGROUND: The purpose of this study is to empirically find out the affecting factors on the willingness to platelet apheresis. METHODS: Data were collected from 505 platelet apheresis donors and college students of major cities in Korea using the self-administered questionnaire. RESULTS: First, the level of positive cognition and affectivity to platelet apheresis donation and satisfaction for service during donation process among platelet apheresis donors were significantly higher than that among students only experiencing whole blood donation and students never experiencing blood donation. Second, valence for external and internal reward and knowledge of platelet apheresis among platelet apheresis donors were significantly higher than that among students only experiencing whole blood donation and students never experiencing blood donation. Third, platelet apheresis donors and students experiencing whole blood donation were more sound in character and more active in volunteer works, and had healthier behavior than students never experiencing blood donation. Fourth, the results of multiple regression analysis showed a statistically significant positive relationship between the willingness to platelet apheresis and valence for internal and external reward, satisfaction for service during donation process, and health behavior. CONCLUSIONS: These findings suggest that strategy for effective public relation and improvement of blood donor satisfaction should be developed in order to enhance willingness to platelet apheresis.