ABSTRACT
Objective To observe the curative effect of sodium phenylacetate (NaPA) on induced differentiation in human glioma cells P 168 in vitro,and preliminarily probe into its mechanism.Methods MTT assay flow cytometry and cell microscope were used to test the changes of P 168 cells treated by different concentrations of NaPA in vitro, and observed ultrastructureal changes of tumor cells.Results NaPA could inhibite the growth of human glioma,show the inhibition effect of time concentration dependent,the flow cytometric was used to find S phase is 11.99% or 13.17% in the NaPA treated P 168 cells and 20.17% in the NaPA untreated P 168 cells. Electron microscope of NaPA treated P 168 cells demonstrated there were rich mitochondria, rough endoplasmic reticulum and elastic filament,but in NaPA untreated P 168 cells,there were numerous scattered polyribosomes appeared.Conclusion NaPA not only inhibit the growth of P 168 haman glioma cells, but also remarkably induce the differentiation of these cells in vitro.
ABSTRACT
Sodium phenylacetate can induce differentiation of tumor cells and has been approved as a drug for the treatment of adults with cancer. In order to explore the immunological mechanism of its antitumor effect, the influence of sodium phenylacetate on HLA class I and II molecule expression in various human tumor cell lines, including breast adenocarcinoma (MCF-7.MUA-453), gastrocarcinoma(MKN-28,MKN-45), ovarian cancer(3AO) and cervical cancer(Hela), was studied with ELISA. The result showed that HLA class II molecule was absent from the surface of MCF-7 cells, but they could be induced after 7 days of continued treatment with sodium phenylacetate. Sodium phenylacetate was found to increase HLA class I molecule expression on the surface of MCF-7 cells, HLA class 1 and II molecule expression on the surface of MDA-453、 MKN-28、 MKN-45、 Hela and 3AO cells. The effect of sodium phenylacetate on HLA class I molecule expression in tumor cells is dose-and time-dependent.
ABSTRACT
The immunosuppressive effect of supernatant of the cultured tumor cells, including breast adenocarcinoma ( MCF-7, MDA-453 ), gastrocarcinoma ( MKN-45 ), cervical cancer ( Hela ) and ovarian cancer ( 3AO ), was studied by MTT assay. The results showed that the supernatant of tumor cell culture suppressed the proliferation of lymphocytes activated with PHA. The supernatant of cultured tumor cells, except that of cultured MDA-453 cells, also suppressed the cytotoxic activity of LAK cells. It is suggested that there are immunosuppressive factors in the supernatant of tumor cell culture. But the immunosuppressive effect of supernatant derived from the cultured tumor cells treated with sodium phenylacetate that is a noncytotoxic differentiation inducer on the proliferation of lymphocytes activated with PHA and the cytotoxic activity of LAK cells , was decreased significantly. It indicates that sodium phenylacetate could decrease the immunosuppressive factors derived from tumor cells.
ABSTRACT
Objective To observe the change of tumor cells on sodium phenylacetate(NaPA) treating G422 glioma mice,and explore its mechanism.Methods Two kinds of mice models(intracranial and muscular G422 glioblasloma cells) were established,and were divided into five groups,among which 3 groups for expe rimental groups,they were received NaPA(1200,800,400 mg/kg?d).In the other two groups,the positive control group was administered BCNU(20 mg/kg) only one time.The negative control group was given saline 24 h later tumor inoculation,these mice bagan to be administered.The experimental group and negative control group were injected NaPA and saline for 14 days respectively.After that,the drug toxicity,the survival period,survival rate of the mice were observed,and the pathology and ultrastructure of glioma in every group were also observed.The muscular tumor mice were sacrificed for measurement of tumor suppression rate.Results NaPA can prolong the life span of the mice with glioma;it has concentration dependent inhibition to glioma proliferation;the pathology and electron microscopy showed the decrease of glioma nuclei fission image treated by NaPA,the increase of rough endoplasmic reliculum and the cell proptosis were found,it demonstrated the tumor cells had differentiation trend.Conclusion NaPA had antitumor effect by inducing glioma cell differentiation and inhibiting the growth of tumor.