Spectrofluorimetric determination of amlodipine in human plasma without derivatization

A rapid and sensitive spectrofluorimetric method was developed for the determination of amlodipine (AD), a calcium channel blocker, in the plasma. The type of solvent, the wavelength range, and the range of AD concentration were selected to optimize the experimental conditions. The calibration curves were linear (r² >0.997) in the concentration range of 0.1-12.5 ppm of AD. The limit of quantitation and limit of detection values for the method for plasma samples were 0.1 ppm and 0.07 ppm, respectively. The precision calculated as the relative standard deviation was less than 3.5%, and the accuracy (relative error) was better than 5.5% (n=6). The method developed in this study can be directly and easily applied for the determination of AD in the plasma without derivatization in plasma.

Método espectrofluorometrico rápido e sensível é descrito para a determinação de anlodipina (AD), um bloqueador de canais de cálcio, em amostras de plasma. O tipo de solvente, a faixa de comprimento de onda e a faixa de concentração foram escolhidas a fim de otimizar as condições experimentais. As curvas de calibração foram lineares (r > 0,997) na faixa de concentração de 0,1-12,5 ppm de AD. Os valores LoQ e LoD do método para amostras de plasma foram 0,1 ppm e 0,07 ppm, respectivamente. A precisão calculada como desvio padrão relativo (RSD) foi menor do que 3,5% e a precisão (erro relativo) foi melhor do que 5,5% (n=6). O método desenvolvido neste estudo pode ser fácil e diretamente aplicado para a determinação de AD sem derivatização no plasma.

Validated spectrofluorimetric method for the determination of atorvastatin in pharmaceutical preparations / 药物分析学报

A rapid,sensitive and simple spectrofluorimetric method was developed for the estimation of atorvastatin.In this method,the native fluorescence characteristics of atorvastatin have been studied in both acidic and basic media.High sensitivity was obtained with 5％ acetic acid at 389 nm using 276 nm for excitation.Regression analysis showed a good correlation coefficient (r=0.9995) between fluorescence intensity and concentration over the range of 1.5-4 μg/mL with detection limit of 0.012 μg/mL.The proposed method was successfully applied to the analysis of atorvastatin in pure and pharmaceutical dosage forms with average recovery of 100.29±0.47％.The results were compared favorably with those of the reported method.

Development and validation of spectrofluorimetric method for estimation of deflazacort in tablets.

A simple and sensitive spectrofluorimetric method has been developed for the determination of deflazacort in pharmaceutical tablet dosage forms. The method was based on the liebermann-burchard reaction, in which the chloroform extract of deflazacort is reacted with acetic anhydride and sulfuric acid to produce strong fluorescence. The resulting fluorophor exhibit excitation and emission wavelengths at 300 and 435 nm, respectively. Linear relationship for the fluorescence intensity was obtained in the concentration range of 0.5 - 10 μg/ml. The method was validated in terms of linearity (0.5-10 μg/ml), repeatability (RSD, 0.99 %), precision (intra-day variation, RSD, 0.239 to 1.287 % and inter-day variation, RSD, 0.360 to 1.830 %) and accuracy (99.12 to 100.28 %). The limit of detection and limit of quantification for deflazacort were found to be 0.15 and 0.45 85.70 μg/ml, respectively. The developed method was successfully used for the assay of deflazacort tablet formulation. The spectrofluorimetric method was found to be simple, sensitive, accurate, precise and economic and can be used for the routine quality control testing of deflazacort in tablet dosage form.