ABSTRACT
Objective To explore the proportional changes of regulatory B lymphocyte cells (Bregs) and suppressor T lymphocyte cell (Ts) in the peripheral blood of patients with Hashimotos' thyroiditis (HT).Method Sixty-one cases of HT and 38 healthy subjects with matched age and gender were recruited.Flow cytometry technology was used to detect the proportional changes of CD19+ CD24+ CD27 + B cells,CD19+ CD24hi CD38hi B cells,CD19+ IL-10+B cells,and CD8+CD28-T cells.Result Compared with control group,CD19+CD24+CD27+B cells in HT group increased significantly(P＜0.05).With the increase of thyroid peroxidase antibody,CD19+ CD24hi CD38hi B cells were decreased significantly.With the increase of antithyrogloblin antibody (TGAb),CD8 + CD28-T cells were decreased markedly (P＜0.05).TT3 was positively related with CD19 + CD24hi CD38 hiB cells while TT4 had a negative correlation with CD8+ CD28-T cells and positive correlation with CD19+ CD24+ CD27+ B cells.Logistic regression analysis showed that TGAb and FT4 were independent risk factors for decreased CD8+ CD28-T cells in lymphocyte percentage(P＜0.05).TSH was an independent risk factor for the decreased proportion of CD19+ CD24hi CD38hiB cell.FT4 was the independent risk factors for increased CD19+CD24+CD27+B cells(P＜0.05).Conclusion The percentage of Ts cells in patients with HT was lowered,while the different phenotypic Bregs cells showed various changes.Thyroid autoantibodies and thyroid function were closely associated with the immunological changes in HT patients.
ABSTRACT
Triptolide (TL) is a major active component extracted from Chinese traditional herb Tripteryginm wil-fordii Hook. In this article, the effects of TL on mixed lymphocyte culture (MLC),suppressor T cell(Ts) activity, delayed type hypersensitivity (DTH) reaction, IL - 2 secretion activity and Th/Ts ratio were evaluated, In vitro TL 0. 5~10 ?g ? L-1suppressed one-way MLC,Lymphocytes induced in first MLC with TL of 5,10 ?g ? L-1 suppressed the second MLC after irradiation with 3000 rad 60Co source. This suggests that TL may induce Ts cell-s. In vivo, DTH reaction sensitized to dinitrofluo-robenzene (DNFB) monitored by the increase in weight of the ears challenged with antigen was suppressed by TL at doses of 0. 12 to 0. 50 mg ? kg-1(ip,qd?5) and the IL -2 activity secreted by spleen cells of these mice was inhibited at doses of 0. 25 and 0. 5 mg ? kg-1. TL 0. 25, 0. 5 mg ? kg-1(ip,qd?8) also had prominent suppres-sive effects on enhanced DTH reaction which was induced by cyclophosphamide(250 mg ? kg-1,ip). Th/Ts ratio of mouse thymus cells were reduced after given 0. 25 mg ? kg-1 of TL for 5 consecutive days. The above-mentioned data suggest that TL has suppressive effects on cellular immunity function and the suppressive mechanism may be related to the suppression of Th cells and IL-2 secretion activity and the induction of Ts cells.