A new dammarane-tye triterpenoid saponin from roots of Panax notoginseng / 中草药

Objective: To study the minor triterpenoid saponins from the roots of Panax notoginseng, which provided basis for the systematic research, quality control and safety evaluation of P. notoginseng. Methods The compounds were isolated and purified by MCI resin, ODS, along with Preparative-HPLC, and the structures were identified by spectroscopic analysis, and comparing with the pubished literature values. Results: Twelve monomeric compounds isolated from the roots of P. notoginseng, were identified as notoginsenoside P1 (1), notoginsenoside T5 (2), ginsenoside Rk3 (3), ginsenoside Rh4 (4), notoginsenoside T3 (5), 20(S)-protopanaxatriol (6), dammar 20 (21),24-diene-3β,6α,12β-triol (7), ginsenoside Rg3 (8), gypenoside XIII (9), ginsenoside Rk1 (10), ginsenoside Rg5 (11), and 20 (S)-ginsenoside Rh2 (12). Conclusion: Compound 1 is a new dammarane-type triterpenoid saponin

Trisomy 21 with t(5;11) Chromosomal Translocation as New Unfavorable Cytogenetic Abnormalities in Pediatric Acute Myeloid Leukemia Type M2: One Case Report of Nine-year Follow-up and Literature Review / 华中科技大学学报（医学）（英德文版）

We report one case of pediatric acute myeloid leukemia type 2 (AML-M2) who presented with karyotypic aberration of trisomy 21 with the t(5;ll) chromosomal translocation.The patient achieved complete remission after two cycles of chemotherapy of daunorubicin,cytarabine and etoposide.Then,follow-up cytogenetic analysis from bone marrow cell cultures demonstrated a normal karyotype of 46,XY.After 9 years,the patient relapsed and the karyotypic abnormalities of trisomy 21 with t(5;ll) reappeared.It was concluded that trisomy 21 with t(5;11) is a new unfavorable cytogenetic aberration in AML-M2.

Assessment of the Pathogenicity Property of Fusarium • raminearum 1 in Balb/C Mice.

An experiment was conducted to investigate the immunologic property, pathogenicity and treatment of Fusarium graminearum infection. Several groups of mice were randomly selected for the following groups: (PC, T1 and T2 were groups of mice that respectively received a 1:1, 1:100 and 1:100,000 fungal dilution while T3, T4, and T5 were groups of mice that respectively received the same concentration but each were treated with Diethylamine Acetarsol (Acetylarsan). A group of mice was included as a negative control (NC),In vitro assays were used to examine the ability of F. graminearum to produce enzymes, which are thought as important virulence indicators. Results revealed the ability of the pathogen to produce collagenase and elastase. In addition, histopathological examination indicated vascular congestion and mild triaditis of the liver. Pulmonary congestion and lymphoid hyperplasia in the spleen were noted. The fungi were recovered from the liver, lungs, spleen and skin of the legs of some experimental animals. Likewise, increase in weight of the spleen doubled as early as the second week (from 49 mg to 80 mg) and progressed up to the fourth week (125 mg) where it tapered off in the untreated group. Similar increase in the weight of the spleen was observed in the treated group (40 mg to 64 mg) but not as great as that in the untreated group (105 mg). Hematological findings showed a lymphocytic count of 1.83 that increased to 3.356, monocyte count of 0.47 that increased to 0.981 and neutrophils increased from 0.399 to 1.698 in untreated groups. Lymphocyte count in the treated group was increased from 1.8 to 3.64, monocytes increased from 0.068 to 0.325 and neutrophils increased from 0.223 to 1.056. High incidence of death was observed in animals that did not receive treatment (PC, T1, and T2) while relatively lower death incidences were exhibited by groups that received diethylamine acetarsol (T3, T4 and T5).

FIP1L1/PDGFRα fusion gene-negative chronic eosinophilic leukemia with t(5; 12)(q31;p13): a case report and review of literatures / 中华内科杂志

Objective To deepen the understanding of chronic eosinophilic leukemia (CEL).Methods The course of diagnosis and treatment in a case of FIP1L1/PDGFRα fusion gene negative CEL was reported. Flow cytometry was used to analyze the immunophenotype of the cells in peripheral blood and pleural fluid. Karyotype was analyzed with G-banding. The expression of FIP1L1/PDGFRα fusion gene was detected by RT-PCR technique. Routine pathological examination of the tissues from bone marrow, lung and spleen were performed. Result A sixteen-year-old girl had severe anemia, fever, splenomegaly,thrombocytopenia and dominant hypereosinophilia lasting for 22 months. Trephine biopsy showed a hypercellular marrow with eosinophilic proliferation and moderate reticular fibrosis. Eosinophilic infiltration was found in lung and spleen and embolism was also found in spleen. She had a clonal chromosomal abnormality t(5;12)(q31;p13). The expression of FIP1L1/PDGFRα was negative. An abnormal clone of T cells expressing CD3-,CD4-,CD8- was found in peripheral blood and pleural fluid, in which the cional T cell accounted for 5.43% and 1.66% of the total lymphocytes respectively. The patient was refractory to treatment with hydroxyurea, prednisone and interferon alpha. She had poor response to a combination of therapy with low dose cytosine arabinoside, mitoxantrone, vincristine, cyclophosphamide, methotrexate and prednisone. She did not respond to imatinib and died of multiple organ failure. Conclusion The present case fulfilled the WHO diagnostic criteria of FIP1L1/PDGFRα(-) CEL which did not respond to routine treatment and imatinib. Allogenic stem cell transplantation should be considered as early as possible in this case. It is noteworthy that clonal CD3-,CD4-,CD8- T-cell abnormality is related to the pathogenesis of CEL.

Detection of Serum IgE Specific to Mite Allergens by Immuno-PCR

BACKGROUND: Although a skin test is the primary option for detecting allergen-specific IgE in clinics, the serum IgE immunoassay is also important because it allows for the diagnosis of allergy without any accompanying adverse effect on the patient. However, the low detection limit of IgE levels by immunoassay may restrict the use of the method in some occasions, and improving its sensitivity would thus have a significant implication in allergy-immunology clinics. METHODS: In this study, we attempted to detect specific serum IgE by using immuno-polymerase chain reaction (IPCR) which combines the antigen-antibody specificity of enzyme-linked immunosorbent assays (ELISAs) with the amplification power of PCR. RESULTS: Our results demonstrated that Blo t5-specific serum IgE can be detected by IPCR with a 100-fold higher sensitivity than ELISA, and cross-reactivity of serum IgE to other mite allergens is able to be analyzed by using only 0.3 microliter of serum sample. Use of real-time IPCR seemed to permit more convenient determination of specific serum IgE as well. CONCLUSION: We believe that IPCR can serve as a valuable tool in determining specific serum IgE, especially when the amount of serum sample is limited.

Establishment of Human Colon Cancer Multi-Drug Resistant Cell Line LS174T/5-Fu / 上海第二医科大学学报

Objective To establish a human colon cancer multi-drug resistant cell line LS174T/5-fluorouracil(5-Fu) and to explore its biological characteristics. Methods A resistant human colon cancer line LS174T/5-Fu was established by stepwise increasing concentrations of fluorouracil selection from the parental cell line LS174T.Drug sensitivity was detected by MTT assay,and the changes of biological characteristics were determined by light microscopy,cell counting,flow cytometry and RT-PCR. Results LS174T/5-Fu cell line was developed after 6 months with stable resistance to 5-Fu and a resistance index of 40.24.The cells exhibited cross-resistance to cisplatin,etoposide,doxifluridine and hydeoxycamptothecin.LS174T/5-Fu cells grew more slowly than LS174T cells,which was longer than LS174T cell line.Cell cycle distribution of LS174T/5-Fu cells was changed compared with parental cells.The percentage of cells in G_(1) and S phase was increased,while in G_(2) phase was decreased.The level of MRP mRNA expression was increased according to the concentration of 5-Fu in resistant cell lines. Conclusion LS174T/5-Fu cells is a reliable multi-drug resistant cell subline of human colon cancer.The successful establishment of this cell subline has laid a solid foundation for further study of the multi-drug resistant mechanism of human colon cancer induced by 5-fluorouracil.

Acute Lymphoblastic Leukemia with Chromosomal Translocation t (5; 14) (q31; q32) and Hypereosinophilia in a Child / 대한소아혈액종양학회지

Hypereosinophilia has been associated with a variety of underlying disorders such as parasitic, fungal and mycobacterial infections, allergic disorders, collagen vascular diseases, or hypereosinophilic syndrome (HES). The association of acute lymphoblastic leukemia (ALL) and symptomatic eosinophilia is rare and only a few cases have been reported. HES probably occurs in less than 1% of all patients with ALL. The chromosomal translocation t (5; 14) (q31; q32) was cloned at the molecular level in ALL with eosinophilia. This translocation joined the immunoglobulin heavy chain region to the promoter region of the interleukin-3 (IL-3) gene in opposite transcriptional orientation. The IL-3 gene translocated with the immunoglobulin heavy chain gene may play a central role in the pathogenesis of this leukemia and the associated eosinophilia. We describe a 8-year-old boy who presented with hypereosinophilia and concurrent ALL with t (5; 14).

The factors relates to the screening tests of colorectal cancer in men

BACKGROUND: Cancer is the second leading cause of death in Korea. Colorectal cancers rank fourth in terms of total cancer mortality. Colorectal cancers have been the focus of screening for early detection. Few current data are available regarding factors associated with participation in cancer screening examinatians in the general population. This study investigated associatians of age, income, educatian, marital status, smoking, drinking, sleeping, presence of a hemorrhoid, and attitudes toward cancer prevention with participation in three cancer screening tests for colorectal cancer. METHODS: To identify the factors associated with participation in cancer screening examinations, 2372 men in a certain campany were surveyed in 1992. Cancer related questians were included to determine the attitudes toward cancer prevention and the history of tests performed that are used for screening for colorectal cancer. RESULTS: Statistically significant (P<0.05) strang predictors of ever having had a specific cancer screening test were as follows : (1) digital rectal examination:abave 40 years old(OR=3.4, 95% CI 2.1-5.4), having a hemorrhoid (OR=2.9, 95% CI 1.7-5.0), and regular exercise (OR=2.1, 95% CI 1.2-3.5) ; (2) fecal occult blood:above 40 years old (OR=4.2, 95% CI 2.5-7.2) and (3) colonoscopy or sigmoidoscopy : above 40 years old (OR=3.5, 95% Cl 2.4-5.2), married (OR=3.5, 95% CI 1.6-7.7), and heavy drinker (OR=1.8, 95% CI 1.2-2.7). CONCLUSIONS: The largest differences among the population for participation in cancer screening examinations involved age. Education and encouragement may persuade men to participate more in screening.

EFFECT OF RIBOFLAVIN ON THE CONCENTRATIONS OF SERUM L-THYROXINE(T_4) AND 3.5.3'-TRHODO-THYRONINE (T_3) OF COLD EXPOSED RATS / 营养学报

In order to evaluate the effect of riboflavin on thyroid hormone levels in the blood of rats during cold exposure, male adult rats were divided randomly into six or eight groups. The group 1,2,5 and 6 were supplemented with 1.5mg riboflavin per kg basal diet, but the group 3, 4, 7 and 8 with 6.0mg riboflavin per kg basal diet. The group 1, 3, 5 and 7 were kept at the cold room (4?0.5) and the group 2,4,6 and 8 at room temperature. The results revealed that supplementation of riboflavin to cold exposed rats could raise the lowered T4 concentration of serum and shorten the time of T4 returned to its original levels. Therefore, riboflayin could hasten cold acclimatization of organism.