ABSTRACT
OBJECTIVE: To study the change rule of specnuezhenide content and antoxidant activity of Ligustri Lucidi Fructus with temperature and time, and determine its validity period. METHODS: Dynamic parameters were established under constant temperature, and specnuezhenide content of Ligustri Lucidi Fructus was determined by HPLC after accelerated test by classical constant temperature method. The shelf life of Ligustri Lucidi Fructus at 25℃ were determined by Arrhenius theory. The antioxidant activities of different samples of Ligustri Lucidi Fructus were evaluated by ABTS assay. RESULTS: The shelf life of Ligustri Lucidi Fructus was forecasted to be 1.83 years at 25℃. The antioxidant activitiy of Ligustri Lucidi Fructus showed a trend of decline after rising first. CONCLUSION: High temperature and storage time would affect the chemical composition of Ligustri Lucidi Fructus, so Ligustri Lucidi Fructus should be kept in the shade for no more than 1.83 years for a good therapeutic effect.
ABSTRACT
The experiment was conducted on sunflower (variety BARI Surjomukhi-2) crop during the month of mid-November, 2014 to mid-March, 2015, in a lysimeter (dimension:1 m X 1 m X 1 m size) to measure crop evapotranspiration (ETc) and determine crop coefficient (kc) values at Irrigation and Water Management Division, Bangladesh Agricultural Research Institute, Gazipur. The study was examined by applying four irrigation at an interval of 10, 15, 20, and 25 days allowing drainage within and adjacent the tank. Results reveals that irrigation at 15 days interval produced the highest yield and was considered suitable for estimating ETc and kc. Seasonal total ETc was found as 270.89 mm. The kc values of sunflower under different ET0 methods for initial, development, mid-season and late season ranged from 0.34 to 0.48, 0.80 to 1.10, 1.06 to 1.55, and 0.27 to 0.36, respectively. Among different methods, P-M method gave relatively higher value than those of other methods and also FAO recommended value. Therefore, this information would be a helpful tool for crop water requirement and irrigation scheduling for similar semi-arid climates.
ABSTRACT
Objective: To prepare novel naringenin-loaded nanostructured lipid carriers (NG-NLC) and investigate their physicochemical properties. Methods: The NG-NLC were prepared by emulsion-evaporation-solidified at low temperature (EESLT) method. Central composite design-response surface methodology (CCD-RSM) was used to optimize the formulation of NG-NLC. Naringenin/lipid materials ratio, glycerol monostearate (GMS)/medium chain triglycerides (MCT) ratio, and emulsifier concentration were identified as key factors which were responsible for entrapment efficiency (EE) and drug loading (LD) of NG-NLC. The mean diameter, DSC analysis, and in vitro release characteristics of NG-NLC were investigated to evaluate their physicochemical properties. Results: After the formulation optimization, the naringenin/lipid materials ratio was 20.77, GMS/MCT ratio was 1.85, and emulsifier concentration was 58.45 mg. The EE of NG-NLC was (80.13 ± 1.45)%, LD was (3.59 ± 0.06)%, average diameter of particles was (134.1 ± 9.1) nm, and polydispersity index (PDI) was 0.152 ± 0.044. In vitro release test revealed that the drug could be sustained-released from NG-NLC in buffer solution (pH 7.4) after a burst release in initial phase. Conclusion: The EESLT method could be employed to prepare NG-NLC, which may lay the foundation for its further clinical application.
ABSTRACT
Objective: To prepare sustained-release honokiol solid lipid nanoparticles (H-SLN). Methods: The solid lipid nanoparticles were prepared by emulsion-evaporation-solidified at low temperature method. The optimum condition was performed based on the orthogonal design to examine the entrapment efficiency, the mean diameter of the particles and the release in vitro. Results: The entrapment efficiency of honokiol in the nanoparticles was 77.1% and the mean diameter of the particles was 159 nm. Conclusion: The emulsion-evaporation- solidified at low temperature method can be used to prepare honokiol solid lipid nanoparticles.