ABSTRACT
Objective:To study the effect of supplementation of calcium and sunshine during pregnancy on serum level of total 25-hydroxyvitamin vitamin D (25-OH-D) . Methods: A total of 151 cases of normal pregnant women were divided into four groups:without calcium and sunshine, without calcium and with sunshine, with calcium and without sunshine, with calcium and sunshine. LIAISON XL chemiluminescence analyzer was used to detect the serum level of 25-OH-D. Results:Compared with the group of without calcium and sunshine, the serum level of 25-OH-D in other groups increased significantly.The calcium deficiency rate of puerpera and neonatal decreased significantly.The reference range was 13.96 ng/ml (2.5%percentile), 39.54 ng/ml (50%percentile) and 73.90 ng/ml (92.5%percentile) .The mean value of 25-OH-D was (41.67 ± 14.72) ng/ml.Conclusion:Proper supplementation of calcium and adequate sunshine during pregnancy can prevent calcium deficiency of puerpera and neonate.The reference range of serum 25-OH-D is slightly lower than that in general population.