ABSTRACT
Objective: To explore the development methods and prelimnary clinical validation of thrombos susceptibility gene chip, and to illustrate a rapid and high-throughput method for detecting the thrombos susceptibility gene mutation. Methods: According to the published gene sequences of thrombotic susceptibility genes in GenBank, the reference probes and special probes were pointed on the glass slide. After ultraviolet crosslinking, thrombois susceptibility gene chip was eatablished. The validity of gene chip was tested by potive reference (mutant gene and normal gene at each detection site) and negative reference (distilled water) as templates, thereby performing PCR reaction. The specificity and sensitivity of gene chip were detected by using the human genome DNA of target sequence proven by sequencing as templates, thereby performng PCR reaction. Meanwhile 150 healthy subjects and 24 thrombosis patients with family hstory of unexplained thrombotic dsease from Jilin province, Henan provnce and Yunnan province were carried out the clinical verification of gene chip. The analyss index was the hybridization signal trength of the correspondng ite. Results: The hybridization results of postive reference and negative reference as templates showed that the specific hybridization signals appeared at the corresponding ites, which indicated that the detection sites of gene chip are effective. The gene chip used to detect the selected mutation sites had specific hybridization signals, suggesng there were good specificity of gene chip. The senstivity of gene chip was 50 - 100 mg · L-1 by testng genomic DNA with stepwise dilution. Eight individuals with thrombosis susceptibility gene mutations were found in 150 healthy subjects. Twenty ndividuals with thrombosis susceptibility gene mutations were found in 24 thrombosis patients with family hstory of unexplaned thrombotic dsease. The statistcs results showed that the detection rate of thrombosis susceptibility gene mutations in the thromboss patients with unexplaned thrombotic disease family history was significantly higher than that of the healthy subjects (P<0.05). Conclusion: The developed thrombosis susceptibility gene chip has great specificity, senstivity, and high detection rate of thromboss susceptibility genes. It has potental values in the early dagnosis and susceptibility sk assessment of thrombotic dseases.
ABSTRACT
Thrombotic diseases in children are much rare compared with adults,and the incidences are increasing in PICU during recent years.The characters of clinical presentations and the results of laboratory tests are different among various kinds of thrombotic diseases in children with venous thromboembolism,arterial thrombosis and thrombotic microangiopathy.The pathophysiological mechanism of thrombotic diseases comprise of coagulation activation and fibrinolysis suppression.Biomarkers of coagulation can reflect the early situation of coagulation and fibrinolysis.Fully and reasonably understanding the changes of various coagulation biomarkers in children is helpful for the early prediction and diagnosis of thrombotic disease.
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Under normal physiological conditions, hemostatic process is a dynamic balance process dependent on many factors.It involves interaction between primary hemostasis (vasoconstriction, platelet clot formation), secondary hemostasis (thrombin generation, fibrin polymerization) and fibrinolysis, and any abnormalities of the three may cause hemorrhagic or thrombotic diseases.Conventional coagulation tests are useful for a limited degree since they only reflect a portion of the coagulation cascade.Thromboelastography (TEG) provides a more complete picture of coagulation status, taking into account more factors involved in the clotting process, including platelet activity and clotting factors, which has been widely used in clinic.In this article, we will briefly discuss clinical applications and new development of TEG.
ABSTRACT
D-dimer is widely used as a kind of coagulation index in clinics and commonly used in the judgement of thrombotic diseases. Recently, studies have found that the D-dimer is related to many clinical diseases in multiple systems, such as the inflammatory, tumorous, etc. diseases can all result in blood coagulation, inducing D-dimer elevation, which may have certain prediction effects on many clinical diseases in determining their diagnosis, prognosis and disease situations. This study has summarized the relationships of D-dimer with many clinical diseases.
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Objective To retrospectively analyze the lower extremity deep vein thrombosis prevention in patients with spinal cord injury (SCI). Methods A total of 115 SCI patients in our department from April to May, 2015 were included. The clinical symptoms, lower limb deep vein ultrasonic testing, laboratory examination were collected to analyze the occurrence, prevention measures, the thrombus location and management of deep venous thrombosis (DVT) in lower limbs. Results Forty-three patients had thromboprophylaxis in other hospitals before admission, and 105 patients in our department after admission, in which, nine cases were with clinical symptoms in other hospitals and three cases in our department. No pulmonary embolism occurred in them. There was no significant difference in most laboratory index-es between patients with DVT and without DVT in lower limbs (P>0.05). Five patients were with DVT in lower limbs in 43 patients who had thromboprophylaxis, and four cases in 72 patients who did not have thromboprophylaxis. No relationship was found between thrombo-prophylaxis and DVT in lower limbs (χ2=0.663, P=0.415). Five patients were with DVT in lower limbs in 53 patients with complete SCI, and four cases in 59 patients with incomplete SCI. No relationship was found between the severity of SCI and DVT in lower limbs in other hospitals (χ2=0.028, P=0.867). Conclusion DVT in lower limbs could be also occurred in patients who accepted thromboprophylaxis. Labo-ratory indexes are inadequate for the prediction and diagnosis specificity of DVT in lower limbs.
ABSTRACT
Plasma D-dimer is one of the degradation products of the cross-linked fibrin hydrolyzed by fibrinolysin and is also a unique metabolite of secondary fibrolysis.The change of its content is a reliable indicator for the identification of the hypercoagulabale state in vivo and primary and secondary fibrinolysis,as well as for the observation of the effectiveness of thrombolytic therapy.In recent years,D-dimer determination has gained new clinical application.
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We have investigated change in platelets and the blood coagulation and fibrinolytic systems as a mechanism of the onset of thrombotic diseases during and after hot-spring bathing. In this study, We examined effect of 10min 42°C and 37°C hot-spring bathing on protein C, protein S and antithrombin III in seven healthy male subjects. The mean values of protein C antigen, protein C activity, total and fee protein S antigens, protein S activity and antithrombin III activity were slightly increased by both methods, while these changes were not statistically significant. Taken together with our previous reports, 10min 42°C hot-spring bathing gives no effect on the blood coagulation system.
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The effect of hot spring bathing on nocturnal blood pressure was investigated in 6 normotensive young individuals at Kusatsu. Blood pressure and heart rate were measured at 1-hour intervals from 18:00 one day until 12:00 the following day. The experiments were performed on 2 consecutive days. Three subjects took a 10-minute 42°C hot-spring bath at 20:00 on the first experimental day but did not on the second experimental day. The order of experiments was inverted in the other 3 subjects. While not statistically significant, the nocturnal blood pressure on the “bathing” day tended to be decreased more than that on the control day. However, there was no difference in the heart rate. These findings may suggest possible involvement of hot spring bathing in the initiation of thrombotic diseases occurred in the morning hours at Kusatsu.