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@#Objective To observe the effect of tilting table exercise and shengmaiyin on alpha 1 adrenogenic receptor (α1AR) in the superior mesenteric artery after spinal cord injury (SCI) in rabbits.Methods 46 rabbits were randomly divided into the sham group (n=6), SCI group (n=10), shenmaiyin group (n=10), exercise group (n=10) and combined treatment (shenmaiyin plus exercise) group (n=10). The rabbit model of SCI was established by complete transection of spinal cord at the T5 spinal segment. Tilting table exercise and shengmaiyin were applied to different groups for 28 days from 4 days after operation. The expression of α1aAR,α1bAR,α1dAR mRNA in the superior mesenteric artery was detected by RT-PCR at 31d after SCI.Results The mRNA expressions of α1aAR, α1bAR, α1dAR subtypes increased to different extent in rabbits of the shengmaiyin group, exercise group and combined treatment group compared with those of SCI group.Conclusion The role of tilting table exercise and shengmaiyin improving orthostatic hypotension after SCI in rabbits may be related to up-regulation of α1aAR, α1bAR, α1dAR mRNA in the superior mesenteric artery.
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@#ObjectiveTo develop an experimental model of orthostatic hypotension (OH) after spinal cord injury (SCI) in rabbits and investigate the effect of tilting table exercise and Shengmaiyin on it. MethodsRabbit model of OH after SCI were induced by complete transection of spinal cord at the T5 spinal segment and raised to 60° head up tilt (HUT) on a tilting table. Tilting table exercise and Shengmaiyin were applied to different groups for 28 d from 4 days after operation. Systolic blood pressure (SBP) and pulse rate (PR) were observed with non-invasive sphygmobolometer of rabbit 3, 10, 17, 24, 31 d after the operation. Liquid chromatography with electrochemical detection(HPLC-ECD) was used to detect the concentrations of plasma norepinephrine (NE) and epinephrine(E) with horizontal position and HUT 60° on the 31st day. ResultsSBP with HUT 60° in rabbits of combined treatment group was significantly higher than that in rabbits of SCI group on the 24th day after operation,and the drop in SBP from horizontal position to HUT60° was significantly lower than that in rabbits of SCI group. SBP with HUT 60° in rabbits of exercise group and combined treatment group was significantly higher than that in rabbits of SCI group on the 31st day after operation. The drop in SBP from horizontal position to HUT 60° was significantly lower in rabbits of Shengmaiyin group, exercise group and combined treatment group than that in rabbits of SCI group on the 31st day after operation. The concentration of E and NE of SCI group, Shengmaiyin group, exercise group and combined treatment group was lower than that of sham group, but there was no significant difference among these groups. ConclusionThe treatment with tilting table exercise and Shengmaiyin can improve OH after SCI partially in rabbits without involving norepinephrine and epinephrine significantly. The period of treatment can be shorten when both therapies used together.
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@#ObjectiveTo establish an experimental model of orthostatic hypotension (OH) after spinal cord injury (SCI) in rabbits and investigate the effect and relevant mechanism of tilting table exercise on OH.MethodsRabbit model of OH was established by completely transecting animal's spinal cord at the T5 segment and putting animal on a 60° tilting table. Tilting table exercise was applied to training group for 28 days starting from 4th day after operation. Systolic blood pressure (SBP) and pulse rate (PR) were observed by non invasive sphygmobolometer of rabbit at 3rd, 10th, 17th, 24th and 31st day after the operation. The concentrations of plasma renin activity (PRA) and angiotensinⅡ (AngⅡ) of different position were detected with radio immunoassay at 31st day after operation.ResultsSBP at 60° head up tilt increased and difference of SBP between two positions decreased significantly in training group at 31st day. The concentrations of PRA and AngⅡ of SCI group and training group were higher than that of sham group. There were no significant differences in the concentrations of PRA and AngⅡ between training group and SCI group.Conclusion T5 complete SCI can induce OH in rabbits. Tilting table exercise can partially improve OH after SCI in rabbit, which may be not related to activation of the circulating renin angiotensin system.
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Objective To develop a medical tilting-table for recovery training of paralysis patients (including paraplegia, hemiplegia and quadriplegia). Methods Renesas 16-bit microcontroller, R8C/25, was used as controller. Danish Linak31 was used to drive the bed. Nixie tube was used to display prescription, angle of tilting table, treating time and music list. Results It worked stable with well-controlled angle. The error was only 1?. The display of nixie tube was good and stable. MP3 played well. Back-up power could provide power supply timely and stably in power failure. Conclusion Medical tilting-table has been successfully applied in clinic.