ABSTRACT
OBJECTIVE:To investigate the technique of purifying the total flavones of Radix Puerariae,so as to provide an experimental basis for extraction and purification of the flavones METHODS:Five purifying methods,including adsorption with macroreticular adsorption resin AB-8,D101,D201 and with polyamide,extration with n-butanol,were adopted,and the amount and purity of total flavones collected and preservation of the components of total flavones were compared RESULTS:By using the above-mentioned five methods,the percentages of the contents of total flavones were 89 4%,83 6%,91 0%,96 0% and 82 9% and the recoveries of products were 79 3%,64 2%,88 0%,55 7% and 77 2% respectively CONCLUSION:Among the five methods,adsorption with AB-8 macroreticular adsorption resin is the best,which may be used for purification of total flavones of Radix Puerariae