ABSTRACT
Objective To optimize separation and purification technology of Zhizichi decoction by macroporous resina . Methods The content of total iridoid glycosides and total isoflavones were determined by ultraviolet spectrophotometry .Tak-ing the content of total iridoid glycosides and total isoflavones as indexes ,the effect of the concentration of sample solution , medicinal herbs on the amount of sample ,concentration and volume of eluent was investigated by single factor test .Results The optimum separation and purification technology was as following :the concentration of sample solution was 0 .1 g/ml ,the volume ratio of resin to material drug was 2∶1 ,the adsorption time was 2 h ,and the sample was firstly eluted with 1 BV wa-ter ,then 6 BV 20% ethanol and 60% ethanol ,and the eluent was collected .Conclusion This optimized separation and purifi-cation technology was reasonable and stable ,and it could be extended to large-scale production applications .
ABSTRACT
Objective To optimize the extracting condition of total isoflavones from Fructus Sophorae by subcritical water extraction.Methods Taking isoflavones yield as index and genistin as control,the technology parameter of subcritical water extraction of Fructus Sophorae isoflavones was optimized by single factor and orthogonal experiment.Results The yield of Fructus Sophorae total isoflavones in the extracts was 23.3% at 130 ℃ for 45 min with the liquid-solid ratio of 12.5 mL/g.Conclusion Compared with traditional extracting methods,subcritical water extraction is a new developing technology in recent years.Its advantage is fast,good-selective.