ABSTRACT
Objective: To investigate the correlation between Ras homolog gene family member A (RhoA) gene in Wnt/PCP signaling pathway and acute exacerbation chronic obstructive pulmonary disease (AECOPD) traditional Chinese medicine(TCM)syndrome, attempting to provide an objective standard for the diagnosis of AECOPD TCM syndrome. Method: The 100 AECOPD patients were collected and divided into 5 groups:phlegm turbid obstructing lung syndrome,, phlegm-heat obstructing lung syndrome, syndrome of orifices confused by phlegm, deficiency of pulmonary and renal Qi, and edema due to yang deficiency, with 20 people in each group. 15 normal people were selected as a normal control group. All patients received fasting hemospasia, using a kit to extract blood total RibonucleicA(RNA) according to instructions. Real-time quantitative polymerase chain reaction (Real-time PCR) was adopted to detect the mRNA expression of RhoA gene in blood of patients with AECOPD TCM syndrome, and to explore the correlation. Result: There was no difference between phlegm-heat obstructing lung syndrome group and syndrome of orifices confused by phlegm group. The mRNA expression of RhoA gene in phlegm turbid obstructing lung syndrome group, phlegm-heat obstructing lung syndrome group, syndrome of orifices confused by phlegm group, deficiency of pulmonary and renal Qi group, and edema due to Yang deficiency group were significantly higher than that in normal group (PConclusion: The significant difference in mRNA relative expression of RhoA gene in Wnt/PCP signaling pathway among the five AECOPD TCM syndrome groups may provide some objective diagnostic criteria for AECOPD TCM syndromes and reveal their disease severity.
ABSTRACT
In order to understand the characteristics of traditional Chinese medicine（TCM） syndrome types, the typical symptoms of different patients and the analysis of professor MA's medication characteristics of the treatment for infertility patients were analyzed. Totally 40 cases of infertile women treated in Xiyuan hospital and TCM out-patient department of China Academy of Chinese Medical Sciences in the past five years were randomly selected to classify their symptoms, signs and the four diagnostic situations in the first diagnosis, summarize syndromes and typical symptoms and analyze the characteristics of medication with Ma. Randomly selected nearly five years in 40 cases of infertile women with China Academy of Chinese Medical Sciences Xiyuan hospital and Chinese medicine out-patient department treatment, first diagnosed by symptoms, signs and the four diagnostic situation. According to the results, kidney yang deficiency： 10 cases (25%), kidney yin deficiency： 6 cases (15%), phlegm dampness： 3 cases (7.5%), liver qi stagnation： 3 cases (7.5%), blood stasis in the uterus： 7 cases (17.5%), deficiency of kidney yin deficiency and phlegm dampness： 1 cases (2.5%), kidney yin deficiency syndrome of liver qi stagnation： 2 cases (accounted for 5%), deficiency of kidney yang deficiency syndrome of liver qi stagnation： 1 cases (2.5%), phlegm dampness syndrome of liver qi stagnation：1 cases (2.5%), kidney yin deficiency syndrome, blood stasis and stagnation in the uterus 2 cases (accounted for 5%), liver qi stagnation syndrome and blood stasis in the uterus 2 cases (accounted for 5%), deficiency of kidney yang deficiency syndrome of liver qi stagnation syndrome and blood stasis in the uterus, 1 cases (2.5%). Typical symptoms were dominated by backache, pain and cold in the lower abdomen, followed by thirst, dry throat, more menstrual blood clots, more petechial hemorrhages at tongue, dysphoria, premenstrual breast pain, irritability, chest distress and greasy in mouth, leucorrhea, semiliquid stool or coprostasis. 40 cases of infertility patients showed obvious curative effect after the treatment of professor Ma's medication.