ABSTRACT
Objective To investigate the effects of Epimedium ,Astragalus ,Radix Puerariae on DMT1 expression in the cere‐bral cortex of APPswe/PS1ΔE9 double transgenic mice model of AD .Methods A total of 60 specific‐pathogen‐free male APPswe/PS1ΔE9 double transgenic mice aged 6 months were equally and randomly assigned to model ,Epimedium ,Astragalus ,Radix puerari‐ae ,compound and DFO groups .An additional 10 6‐month‐old C57BL/6J mice served as negative control group .Using immunohisto‐chemistry and molecular biology methods to investigate the effects of a compound combining the effective components of Epimedi‐um ,Astragalus ,Radix puerariae on DMT1 expression in the cerebral cortex of APPswe/PS1ΔE9 double transgenic mice model of AD . Results Immunohistochemical staining results revealed that DM T 1 positive cell did not show in negative control group .DM T1 ex‐pression was higher in model group compared with the negative control group .DMT1 expression was lower in the compound and deferoxamine groups than in the model group .No significant difference was detected in DM T 1 expression between deferoxamine and compound groups .RT‐PCR ,Western blot and immunohistochemical staining results showed no significant difference .Conclusion These compounds can downregulate DMT1 expression and inhibit iron overload in the cerebral cortex of mice with Alzheimer′s dis‐ease ,reduce iron overload induced impairment of the central nervous system .
ABSTRACT
Uveitis is an important autoimmune disease.Human leukocyte antigen (HLA)-B27-associated anterior uveitis is the most common form of anterior uveitis.HLA-B27-associated anterior uveitis has distinct ocular,systemic,and genetic features.It attacks young subjects and leads to the damage of visual function due to the recurrence and complications.However,the pathologic mechanism of HLA-B27-associated anterior uveitis is still not thoroughly understood,and effective therapy is still unavailable.There are currently many experimental researches which attamp to study the etiology and develop an effective therapy for HLA-B27 acute anterior uveitis.Thus,various animal models of acute anterior uveitis such as endotoxin-induced uveitis and HLA-B27 transgenic animals including rat and mouse are established.Different therapy regimens have been designed both in clinical trial and experimental study and aquired promising achievements.Epidemiology,pathologic mechanism,different animal models and potential new therapies such as anti-TNFα,oral associated peptides tolerance and gene therapy are overviewed.