ABSTRACT
In this paper,the effects of active fractions of Ferula ferulaeoides on the growth and apoptosis of human gastric cancer cell MGC-803 transplantation tumor were systematically studied. The subcutaneous ectopic transplantation tumor model was established in human gastric cancer MGC-803 nude mice by cell suspension implantation method. The anti-tumor rate and organ index were used to evaluate the anti-tumor effect of the active fractions of F. ferulaeoides on the tumor-bearing nude mice. HE staining,TUNEL staining,RT-PCR,Western-blot and ELISA were used for pathological examination,apoptosis observation,and detection of apoptosis-related genes,proteins and cytokines expression. The results showed that as compared with the model group,the low,medium and high doses of the active fraction of F. ferulaeoides had inhibitory effects on xenografts in nude mice,respectively,in a dose-dependent manner; the apoptotic ratio was increased with the increase of drug concentration. As compared with the model group,F. ferulaeoides could down-regulate the expression of survivin mRNA in nude mice,and the protein expression levels of Bax,Bcl-2,caspase-3 and caspase-9 in tumor tissues of nude mice could be increased to different degrees in F. ferulaeoides groups. The contents of IL-10 and TGF-β1 in plasma of nude mice were decreased in high dose group of F. ferulaeoides active fractions. The results indicated that F. ferulaeoides can significantly inhibit the growth of human gastric cancer MGC-803 subcutaneously transplanted tumor,and its mechanism may be related with down-regulating the expression of survivin mRNA,and up-regulating the expression of apoptosis-related proteins Bax,caspase-3 and caspase-9.
Subject(s)
Animals , Humans , Mice , Apoptosis , Caspase 3 , Metabolism , Cell Line, Tumor , Cytokines , Metabolism , Ferula , Chemistry , Mice, Inbred BALB C , Mice, Nude , Neoplasm Transplantation , Plant Extracts , Pharmacology , Proto-Oncogene Proteins c-bcl-2 , Metabolism , Stomach Neoplasms , Drug Therapy , bcl-2-Associated X Protein , MetabolismABSTRACT
OBJECTIVE:To study the inhibitory action of Sarcandra glabra injection (ZJF) on tumor growth of SGC-7901 transplantation tumor in nude mice and its induction on apoptosis. METHODS:SGC-7901 transplantation tumor nude mice model was established then the model mice were assigned to blank,positive control,ZJF high-dose and low-dose (3,1.5 g?kg-1) groups. After treatment with ZJF for 14 days,the indexes of thymus and spleen,numbers of peripheral red blood cells,white cells and platelet were measured; the tumor-inhibition rate was computed; the ultrastructure of tumor tissues was observed,and the apoptosis of implanted tumor cells was observed. RESULTS:As compared with blank group,no significant change was noted in the indexes of thymus and spleen and peripheral hemogram. The differences between high-dose ZJF group and low-dose ZJF group were greatly significant in both the inhibition rate of SGC-7901 transplantation tumor (42.8% vs. 39.9%) and the apoptotic rate of tumor tissues [(9.3?0.15)% vs.(8.18?0.08)%].One or more apoptotic bodies formed after medication. CONCLUSION:ZJF can promote the presence of apoptotic body and induce apoptosis thus inhibiting the growth of SGC-7901 transplantation tumor in nude mice; however,it had little impact on the thymus and spleen and peripheral hemogram.