ABSTRACT
The study was conducted on 19 adult dogs referred for treatment of fracture in diaphyseal region of three long bones, viz, radius-ulna, femur and tibia-fibula. These dogs were divided randomly into three groups viz T1, T2 and T3. In group T1, the diaphyseal fractures of the long bones were immobilized by an internal fixation technique using reconstruction plate or locking compression plate. In group T2, β- tri calcium phosphate granules were incorporated at fracture site after fixation. In group T3, one milliliter of PRP was administered locally at fracture site just before the closing the first suture line. Clinical and radiographic examination were also conducted during the study. There was a significant reduction in mean inflammation score at 7th day in groups T2 and T3 and at 30th day than 0 day in all the groups. At 90th day, the mean radiographic score was highest for group T3 and it was followed by the score in group T2. At day 90th, a radiograph in both the views was not able to delineate the granules from osseous tissue. The mean weight bearing score was also higher in group T3 than in group T2 at 30th, 60th and 90th post operative day showing that restoration of the function was better and earlier in groups T2 and T3 than in group T1
ABSTRACT
The aim of this study was to evaluate the indicators of osteogenesis, cytotoxicity and genotoxicity of an experimental beta tri-calcium phosphate (experimental ß-TCP) compared with two other bone substitutes: bovine hydroxyapatite (HA) (Bio-Oss® - Geistlich) and beta tri-calcium phosphate (ß-TCP - Bionnovation). The cell viability and genotoxicity were measured by MTT and MNT assay, respectively. The indicators of osteogenesis were analyzed by alkaline phosphatase activity, total protein content, and calcium deposition. The MTT and MNT assay showed that none of the tested materials was cytotoxic nor genotoxic. Concerning the indicators of osteogenesis, it was observed that cells in contact with all the materials were able to induce the osteogenesis and this process was influenced by the period of the cell culture in contact with bone substitutes. Based on the results of this study, it was concluded that this experimental ß-TCP appears to be a promising material as a bone substitute.
O objetivo deste estudo foi avaliar os indicadores da osteogênese, citotoxicidade e genotoxicidade de um beta-tricálcio fosfato (ß-TCP experimental) comparado com dois outros substitutos ósseos : Hidroxiapatita Bovina (HA) (Bio-Oss® - Geistlich) e beta-tricálcio fosfato (ß-TCP - Bionnovation). A viabilidade celular e genotoxicidade foram mensuradas pelos ensaios MTT e MNT, respectivamente. Os indicadores da osteogênese foram analisados pela atividade de fosfatase alcalina (ALP), conteúdo de proteína total, e deposição de cálcio. Os ensaios MTT e MNT mostraram que nenhum dos materiais testados foi citotóxico ou genotóxico. Em relação aos indicadores da osteogênese, foi observado que as células em contato com todos os materiais foram capazes de induzir a osteogênese, e que esse processo foi influenciado pelo período da cultura celular em contato com os substitutos ósseos. Baseado nos resultados desse estudo, conclui-se que este ß-TCP experimental parece ser um material promissor para ser utilizado como substituto ósseo.
Subject(s)
Cattle , Osteogenesis , Indicators (Statistics) , Genotoxicity , HydroxyapatitesABSTRACT
Aims: The present work is aimed to find out the enzymatic activities and phosphate solubilizing efficiency of indigenous rhizobia confined to rice fallows. Study Design: In this experiment we maintained random block design (RBD). Place and Duration of Study: This work was carried out in the Department of Botany and Microbiology, Acharya Nagarjuna University between October 2012 and December 2013. Methodology: In this study, we have isolated 19 Rhizobium strains collected from the healthy root nodules of Vigna mungo cultivated in rice fallows on yeast extract mannitol agar (YEMA) medium. The strains were confirmed as Rhizobia by using Gram staining, growth on YEMA with congo red, growth in Hofer’s alkaline broth, growth on glucose peptone agar, acid production, ketolactose test and nodulating ability was tested on homologous hosts by plant infection tests. Phosphate solubilization ability of the isolated Rhizobium strains were carried out Pikovskaya’s agar medium. Results: Eight out of 19 strains tested for phosphate solubilizing ability on Pikovskaya’s agar medium containing tri calcium phosphate (TCP) as insoluble phosphate source showed zone of TCP solubilization. The strain VM-2 exhibited maximum solubilization after 48h of incubation, while least activity was found with VM-11. Effect of different carbon and nitrogen sources on phosphate solubilizing ability of Rhizobial strains was tested and maximum phosphate solubilization (799μg/ml) by VM-2 was observed when glucose and ammonium sulphate were used as carbon and nitrogen sources. Conclusion: In this study it is concluded that along with symbiotic nitrogen fixtation, some Rhizobium species were found to be involved in phosphate solubilization and this ability of phosphate solubilization by the Rhizobium strains can be exploited as PGPR.
ABSTRACT
Objective Bio-ceramics has been widely utilized as a common type of bone repair material.The research was designed to focus on preparation and characterization of porous TCP.Methods By using hydroxyapatite (HA) as the raw materials,and adding calcium phosphate bio-glass to adjust the ratio between calcium and phosphate,in order to achieve making porous TCP bio-ceramic scaffolds during high temperature heating process.Results Test results showed that by applying this method,TCP scaffolds had significantly higher mechanical strength then those were made from powdered TCP,and its compressive strength reached 9.11 MPa.Conclusion Tri-Calcium Phosphate (TCP) has excellent biocompatibility and it is also biodegradable.In the physiological environment,it is able to be gradually decomposed,absorbed and to promote bone growth ultimately,it is capable of replacing the damaged bone components with brand new bone tissues.