ABSTRACT
Objective@#To investigate the effect of Kamistad gel on oral ulcer healing and the expression of tumor necrosis factor α (TNF-α), interleukin 6 (IL-6) and epidermal growth factor (EGF) after oral administration in ulcer tissue of rats and to provide animal experimental data for the clinical application of Kamistad gel.@*Methods@#The oral ulcer rat model was established by chemical cauterization. The rats were randomly divided into four groups: Kamistad group (Kamistad gel), befuxin group (befuxin gel), lidocaine group (lidocaine cream), blank control group (normal saline), with 12 rats in each group. The ulcer area of the rats in each group was measured before and 1, 3 and 5 days after treatment; at 1 day after treatment, the duration of swabbing behavior within 3 minutes of intraoral capsaicin infusion was recorded to evaluate the degree of pain; the ulcer tissue was collected at 5 days after treatment, and the histopathological changes were observed by HE staining, the expression of TNF-α, IL-6 and EGF in the ulcer tissue was detected by immunohistochemistry and ELISA.@*Results@#At 1 day after treatment, the duration of mouth wiping induced by capsaicin was significantly shorter in the Kamistad group than in the blank control and befuxin groups (P < 0.05), but there was no significant difference between the Kamistad and lidocaine groups (P >0.05). At 5 days after treatment, the ulcer area was significantly smaller in the Kamistad group than in the blank control and lidocaine groups (P < 0.05), and there was no significant difference between the Kamistad and befuxin groups (P >0.05). At 5 days after treatment, H&E staining of the oral ulcer tissue sections showed significantly reduced levels of inflammatory cells and significantly proliferated fibroblasts and better epithelial hyperplasia in the Kamistad group compared with those in the lidocaine and blank control groups, and there were no differences between the Kamistad and befuxin groups. At 5 days after treatment, the expression levels of TNF-α, IL-6 and EGF in the ulcer tissue of rats in each group were significantly different (P < 0.05). Compared with the blank control and lidocaine groups, the expression of TNF-α and IL-6 was significantly decreased and the expression of EGF was significantly increased in the Kamistad group (P < 0.05); there were no significant differences in the expression of the above three factors between the Kamistad and befuxin groups (P > 0.05).@*Conclusion@#Kamistad gel exhibited anti-inflammatory, analgesic and healing effects on experimental oral ulcers.
ABSTRACT
@#AIM: To investigate the relationship of TNF-α and IL-6 with the pathogenesis and development of the neovascular glaucoma(NVG). <p>METHODS: Totally 64 eyes of 64 NVG patients, 58 eyes of 58 primary open angle glaucoma(POAG)patients and 56 eyes of 56 age-related cataract(ARC)patients were enrolled in our Hospital from January 2017 to January 2018. The periphery blood was collected from all the patients and 0.3mL aqueous humor was collected during the surgery from each eye. The levels of TNF-α and IL-6 in serum and aqueous humor of the three groups were measured by ELISA.<p>RESULTS: The concentrations of TNF-α and IL-6 in aqueous humor and serum in NVG patients were higher than those in POAG patients as well as in ARC patients, with significant differences among the three groups(<i>P</i><0.05). The TNF-α and IL-6 in aqueous humor and serum in NVG patients were significantly positively correlated(<i>r</i>=0.642, 0.415; <i>P</i><0.05). <p>CONCLUSION: The aqueous and serum TNF-α and IL-6 are considerably higher in NVG patients than those in POAG patients and ARC patients, suggesting that TNF-α and IL-6 play important roles in the pathogenesis and development of the NVG.