ABSTRACT
After a single oral dosed mg/kg) of nitroquine to the mice infected with Plasmodium yoelii,the morphological changes of the parasites were studied with optical and electron microscopy.Enlarged nucleus and some red granules scattered over the cytoplasm in the late trophozoites were observed under optical microscope,which may correspond to the autophagocytic vacuo-les or membranous residual bodies seen under electron microscope.Thirty minutes after the drug administration,the number of mitochondria with matrix cavitation was increased,the endoplasmic reticulum dilated,and ribosomes separated,detached,or disaggregated.Then the pelliculous complex and nuclear membrane of the parasites proliferated to form multi-layered structure with spiral curv?s or myelin sheath-like structure in the cytoplasm.The nuclear membrane was swollen and proliferated and the perinuclear cisterna was widened.In the late stage of drug action,the structure of the parasites was broken down to form a large number of autophagocytes.The findings indicate that nitroquine interferes with the structure and function of the cell membrane,cytoplasm and nucleus of malaria parasites and exerts its anti-malarial effects from many aspects.
ABSTRACT
The article reports the result of the observation on the ultrastructure of the tegument and digestive tract of Schistosoma japonicum schistosomula cultured 130 days in vitro in 841 medium,and the 42-day-old adults from infected animal cultured 23 days in 851 medium by transmission electron microscopy.The results showed that the ultrastructure of the tegument including sensory papillae and flame cells was normal.The matrix seemed to have no vacuolization.The surface of the esophagus was highly rugous.Many laminae and lipid droplets filled the lumen of the digestive tract and the tubules-like depressions from basal lamina were occured.All these structures seemed to have less variation as compared with the prior authors' data from normal worms.It is considered that the above two media which we cesigned were suitable for the growth and development of the schistosomes due to the tegument and digestive tract maintained their metabolic function in the long-term cultivation in vitro.(Figs 1-10).
ABSTRACT
The Weibel-Palade-like body were found in the cytoplasm of the growth hormone and follicullar - stellate cells of rabbit adenopypophsis. The body is surrounded by a single unit membrane. In this body, there are numerous of microtubules which can be divided into two groups depending upon their diameters.Yet, it is uncertain what the virtual function is and where the bodies come from. However, according to the experimental observations, it is suggested that the body serve as a mechanical power device which has relations with the regional distribution of the organelles. The intranuclear microtubules and intranuclear tubules were also found in the growth hormone cells. The diameters of these intranuclear microtubules range from 25 - 30 nm, and the intranuclear tubule about 78 nm. The fomer seems to be connected with the heterochromatin granules, and may be. a transitional pattern from heterochromatin to euchromatin, but their importance remain to be clarified.