Detection of uronic acid of Lactobacillus rhamnosus PN04.

Lactobacillus rhamnosus is known as species that had many benefits in pharmaceutical, dairy food industry. To supply more applications for life, the detection of uronic production of Lactobacillus rhamnosus PN04 was done. After the extracellular components of Lactobacillus rhamnosus PN04 culture were precipitated with 1% cetyl pyridium chloride, the precipitants were used to check the uronic acid production capacity by carbazole assay according to BP98. As a result, the highest uronic acid yield was produced at 96 h cultivation. The maximum of uronic acid was about 74mg/L. The study was the first report the uronic acid existing in Lactobacillus rhamnosus PN04.

Composition and Partial Structure Characterization of Tremella Polysaccharides

Heteropolysaccharides isolated from liquid cultures of nine Tremella species contained 0.3 to 1.2% protein, 2.7 to 5% ash, 0.9 to 3.4% acetyl groups, 76.5 to 84.2% carbohydrates and trace amounts of starch. The polysaccharides in aqueous solution were slightly acidic (pH 5.1 to 5.6). They consisted of the following monomeric sugars: fucose, ribose, xylose, arabinose, mannose, galactose, glucose and glucuronic acid. The backbones of the polysaccharide structures consisted of alpha-(1-->3)-links while the side chains were beta-linked.

New simple method to detect uronic acids in polysaccharides / 中草药

Object To develop a simple and reliable method to detect the presence of uronic acids in polysaccharides Methods Uronic acid containing polysaccharides and standard glucons were subjected to acid hydrosis for 2 h and 4 h respectively. Samples of the products, before and after hydrolysis were analyzed by TLC using standard uronic acid as the control and aminobenzen alizaric acid as the chromogenic agent, to compare the sensitivities in the detection of uronic acid used in the present conventional analysis. Results The fluorescent spots used as criterion in traditional TLC method were not shown by uronic acids, but by CF 3COOCH 3 and other by products in reaction 0 5 ?g of uronic acid could be detected with our method, without false positive reaction Conclusion The traditional TLC method seemed to lack theorectical background and may mislead structural study The present method in more reliable to detect uronic acids in polysaccharides by analyzing the complete hydrolytic products of polysaccharides using TLC with standard uronic acids as control and aminobenzene alizaric acid as chromogenic agent