ABSTRACT
La potencialidad terapéutica de fármacos se averigua mediante estudios bioquímicos y celulares que nos hablan de sus acciones sobre vías de señalización y receptores. Sin embargo, en algunas enfermedades -por ejemplo, enfermedades neurológicas conocidas como "desórdenes del movimiento"-, los bioensayos realizados miden las acciones farmacológicas mediante valoraciones conductuales en modelos animales de las mismas. No se han logrado bioensayos que correlacionen la acción terapéutica de fármacos sobre la actividad del tejido vivo. Se puede medir la actividad de decenas de neuronas mediante imagenología de calcio en tejido vivo. Ciertos parámetros de esta actividad neuronal registrada in vitro reflejan su estado patológico, así como la acción terapéutica de fármacos determinados. No hay un sistema integrado orientado a estos bioensayos, por lo que se combinan diferentes equipos comerciales de manera independiente con costo final de alrededor de 100,000 USD. Presentamos un prototipo de un sistema integral encaminado a realizar este tipo de bioensayos: microscopía de epifluorescencia con calidad suficiente para adquirir y medir cuantitativamente la actividad celular del tejido vivo registrada in vitro pero de costo 10 veces menor -alrededor de 10,000 USD-. Se pueden realizar satisfactoriamente bioensayos funcionales de uso potencial en la industria farmacéutica, investigación y docencia.
The therapeutic potential of drugs is determined by biochemical and cellular studies that inform us about their actions on signaling pathways and receptors. However, in some diseases -for example, neurological diseases such as "movement disorders"-, bioassays measure the pharmacological actions by evaluating behavior in animal models of the diseases. There are no bioassays that correlate drug therapeutic actions on living tissue. The neural activity of several neurons can be measured by using calcium imaging on living tissue. Certain parameters of the recorded neuronal activity in vitro reflect the pathological state and the therapeutic actions of specific drugs. There is no integrated system oriented to these bioassays, so different commercial equipment has to be integrated independently with costs about 100,000 USD. We present a prototype of an integral system aimed to perform bioassays in vitro: epifluorescence microscopy with enough quality for the acquisition and quantitative assessment of cell activity recorded in the living tissue with costs around 10 times less -about 10,000 USD-. It allows successfully functional bioassays of potential use in the pharmaceutical industry, research an education.
ABSTRACT
Se desarrolla e implementa un sistema basado en instrumentación virtual para la adquisición y procesamiento de señales fisiológicas para ser empleado como herramienta para mejorar el proceso de enseñanza-aprendizaje que se lleva a cabo en los laboratorios de fisiología de las facultades de Medicina de nuestro país. El hardware del sistema comprende un amplificador de bio-potenciales de cuatro canales, una tarjeta de adquisición de datos y un computador personal (PC) que permite la interfase con el usuario. Para el desarrollo del software se empleó el lenguaje de programación gráfico LABVIEW 6.1. Se implementaron prácticas de laboratorios empleando principalmente las señales fisiológicas: electrocardiográficas y electromiógraficas. Como resultado del trabajo se implementó un prototipo que funciona con los requerimientos técnicos exigidos para realizar prácticas docentes en un laboratorio de fisiología. Entre las conclusiones del trabajo podemos nombrar: es factible el diseño e implementación de instrumentos virtuales con fines educativos para ser empleados en laboratorios con las mismas capacidades de instrumentos equivalentes producidos en países desarrollados, y la necesidad de que estos instrumentos puedan producirse en serie para beneficiar a un mayor número de estudiantes de Medicina.
It is developed and it implemented a system based on virtual instrumentation for the acquisition and physiological signal processing to be used like tool to improve the education process learning that are carried out in the laboratories of physiology of the medicine faculties of our country. The hardware of the system includes/understands a bio-potential amplifier of 4 channels, a card of data acquisition, and a personal computer (PC) that the interphase with the user allows. For the development of software the graphical programming language LABVIEW 6.1 was used. Practices of laboratories were implemented mainly using the physiological signals: electrocardiograficas and electromiograficas. As resulting from the work implemented a prototype that works with the technical requirements demanded to make educational practices in a physiology laboratory. Between the conclusions of the work we can name: It is feasible the design and implementation of virtual instruments with educative aims to be used in laboratories with the same capacities of produced equivalent instruments in developed countries, and the necessity that these instruments can take place in series to benefit to a greater number of medicine students.
ABSTRACT
This paper introduces the working principle of the blood circle treatment system outside the body. A monitoring instrument of plasma leakage suited for the blood circle treatment system outside the body is developed based on the absorption spectrum experiments of plasma leakage. Photoelectric detection technology and virtual instrumentation are utilized in the development. A series of detecting experiments of waste solution containing plasma with continuously changing concentration show the monitoring system possesses a relatively high sensitivity. Moreover, the experiments of continual detection with plasma concentration at one certain point indicate the monitoring system has a quite good stability. The monitoring instrument is adapted to dynamically detecting the plasma leakage when the blood circle treatment system outside the body is working.
ABSTRACT
Virtual instrumentation makes great progress in instrument development. LabVIEW is a graphical programming language that uses icons instead of lines of text to create applications. It has been used widely in developing virtual instrument. The application of virtual instrument to medical equipments presented, this paper introduces how to drive common DAQ system and its several implement methods, builds a shared library adapting to AC6010, and develops a low-cost and good-capability DAQ system, which has been utilized to detect biomedical signal with a optic-electric detecting system.