Microorganisms used for bioleaching of metals from typical solid wastes and their leaching mechanism: a review / 生物工程学报

Typical solid wastes contain many metal resources, which are worthy of recycling. The bioleaching of typical solid waste is affected by multiple factors. Green and efficient recovery of metals based on the characterization of leaching microorganisms and the elucidation of leaching mechanisms may contribute to the implementation of China's "dual carbon" strategic goals. This paper reviews various types of microorganisms used for leaching metals from typical solid wastes, analyzes the action mechanism of metallurgical microorganisms, and prospects the application of metallurgical microorganisms to facilitate the application of metallurgical microorganisms in typical solid wastes.

Comparative Roles of HBB5 Biosurfactant and Poultry Wastes in Polyaromatic Hydrocarbon Biodegradiation of Crude Oil-contaminated Sediment

The comparative study of poultry wastes- and HBB5 biosurfactant-mediated polyaromatic hydrocarbon biodegradation in sediment polluted with crude oil were investigated. The experiments were carried out for a period of 28 days by monitoring pH, nitrate, phosphate, polycyclic aromatic hydrocarbon and microbiological parameters using standard procedures. The pH values obtained ranged between 6.21 and 6.93 in days 1 and 28 for the most effective treatment recipes. Generally, there was depletion in the concentrations of nitrate and phosphate for all set ups, but the most effective recipe witnessed highest reduction. For the polycyclic aromatic hydrocarbons, the recipe with highest limiting nutrients depletion also recorded the most hydrocarbon loss, and yet highest increase in density of hydrocarbonoclastic bacteria and fungi. The sample containing polluted sediment + poultry wastes + HBB5 biosurfactant recorded PAH values of 1932.6472ppm on day 1 and 481.2272ppm on day 28. Total hydrocarbon-utilizing bacterial counts ranged from 1.48×104 cfu/g to 9.70×106 cfu/g, while hydrocarbon-utilizing fungal counts ranged between 2.30×103 cfu/g and 3.90×105 cfu/g. From the results obtained, poultry wastes combined with HBB5 biosurfactant recorded the highest efficiency in the biodegradation of polycyclic aromatic hydrocarbons in sediments, and HBB5 biosurfactant in isolation recorded higher degradation efficiency for polyaromatic hydrocarbons than the degradation effect mediated by poultry wastes alone. It is therefore recommended that a combination of surface-active agent, nutrient amendment source and viable microbial biomass be adopted and employed as potent recipe for the degradation of polyaromatic hydrocarbons in crude oil-contaminated sediments.

Evaluation of Natural Pigments Extracted from Tomato Wastes and the Possibility of their Encapsulation

This study aimed to evaluate the best methods from extracting natural pigments from tomato fruit wastes by four techniques used to extract lycopene and β-carotene each of them consisting of three solvents: ethanol, acetone and hexane in the following ratios (1:1:1), (2:1:1), (1:2:1) and (1:1:2) ml respectively. We studied too the possibility of encapsulation by freeze drying with a mixture of gelatin and gum Arabic as a carrier in nine microcapsules differing with respect to the total encapsulant (E) (2.5, 5.0, and 7.5%) and core (C) concentrations, the latter varying in relation to the total weight of encapsulant (25, 50, and 75%). The nine microcapsules were coded as follows: (1) E2.5/C25; (2) E2.5/C50; (3) E2.5/C75; (4) E5.0/C25; (5) E5.0/C50; (6) E5.0/C75; (7) E7.5/C25; (8) E7.5/C50; and (9) E7.5/C75. We found that the best solvent mixture for the extraction process was 1:1:2, and the most efficient microcapsules were E5.0/C75, E5.0/C50 and E2.5/C25. By studying the stability of the best three microcapsules when exposed to different values of heat, light, oxygen and pH, it was found that the most stable of them was E5.0/C75, followed by E5.0/C50 and then E2.5/C25. It was therefore recommended that further future studies are needed to evaluate the potential of this microcapsule as a natural additive in food, pharmaceuticals and cosmetics.

Batch Mode Biomethane Production from Anaerobic Digestion of Palm Oil Mill Effluent (POME) with Cassava Peels and Cabbage Waste

Introduction: Biomedical waste (BMW) generated is a matter of great concern as it consists of high levels of hazardous waste. The lack of segregation and disposal policy in India leads to use of such infectious waste in day-to-day life [1]. Lack of awareness about segregation of Biomedical Waste (BMW) at source is a major cause for Hospital Acquired Infection (HAI) and increased risk of contamination of hospital and external environment. Materials & Methods: To address the problem, we decided to train interns and health care workers to increase their awareness about BMW segregation and thereby reduce HAI. The program was run in 3 phases. In the 1st phase need assessment was done as to how important and effective this program would be in controlling HAI. The 2nd phase comprised of identifying the stake holders in the program who will play the key role in implementing the program effectively. The 3rd phase of Biomedical Segregation Program BMWSP focused on the outcomes as enumerated in the Kirkpatrick model [2], viz., participants’ reaction, learning, change in behavior and results. Measuring the impact of the program on the learners at these four levels revealed the effectiveness of the program. Results: To overcome the shortfalls in the Kirkpatrick model we used Logic model which took care of the instructional design. Logic model is better suitable for evaluating BMWSP as it considers inter-relation between constituents in a sequence and their effect on the process as well as product providing the necessary roadmap from the planned work (inputs and activities) to the intended results (outputs, outcomes and impact). It is a sequence of events connecting each other starting with Resources / Inputs, Activities, Outputs, Outcomes and Impact. The model helped medical interns and dental students to understand the importance for BMWSP and also made a significant difference in its implementation. Conclusion: The program helped in making the health care staff more aware about BMWSP. It also increased their knowledge, and helped in implementation of the program. This will significantly reduce HAI and overall benefit the society.

Bioethanol Production from Various Lignocellulosic Materials by Encapsulated Saccharomyces cerevisiae NCIM 3095

Bioethanol has a greater promise for environmental safety and energy security than fossil fuels. The alternate source required to meet the fuel's requirements can be provided by bioethanol. Untapped sugar-rich sources, like cellulose-rich household wastes, industrial wastes, and agricultural wastes, can all be used to make bioethanol at a minimal cost. The study's objective was to determine whether saccharomyces cerevisiae cells from the encapsulated NCIM 3095 strain of Saccharomyces cerevisiae could be used to make low-cost ethanol from a variety of lignocellulosic wastes, including newspaper, banana leaves, gram straw, soybean straw, and cow dung. To reduce bacterial contamination and serve as an external growth stimulator, benzathine penicillin G and ammonium sulfate were added to each sample broth containing calcium alginate-encapsulated yeast cells. The samples were fermented for ten days. The ethanol content was evaluated every three days. The largest yield of bioethanol was produced by soybean straw (10.0%), while the lowest was by cow dung (4.0%).

Pachira aquatica fruits shells valorization: renewables phenolics through analytical pyrolysis study (Py-GC/MS) / Valorização das cascas dos frutos da Pachira aquática: fenólicos renováveis através de estudo analítico de pirólise (Py-GC/MS)

This research valorized Pachira aquatica Aubl.'s fruit shells (PAS) through its energetic characterization and flash pyrolysis for biofuels or chemicals production. The characterization was performed through proximate and ultimate analysis, bulk density, higher heating value (HHV), hemicellulose, cellulose and lignin content, thermogravimetric analysis and absorption spectra in the infrared region obtained by Fourier-transform infrared spectroscopy technique (FTIR). The analytical flash pyrolysis was performed at 500°C in a Py-5200 HP-R coupled to a gas chromatograph (Py-GC/MS). The PAS biomass presents potential for thermochemical energy conversion processes due to its low moisture and ash content, 76.90% of volatile matter, bulk density of 252.6 kg/m3 and HHV of 16.24 MJ/kg. Flash pyrolysis products are mostly phenols or light organic acids derived from the decomposition of polysaccharides. Results confirmed the potential of PAS to produce bio-phenolics, such as 4-methoxyphenol which is an important active ingredient for skin depigmentation used in drugs and cosmetics, and as phenolic extract that can be used as a precursor to resins, applications that convert this forest waste into bio products for industry into a green circular economy.

Este trabalho teve como objetivo a valorização das cascas dos frutos da Pachira aquatica Aubl. (PAC) através da sua caracterização energética e pirólise flash para produção de biocombustíveis ou produtos químicos. A caracterização foi realizada através de análises imediata e final, densidade aparente, poder calorífico superior (PCS), conteúdos de hemicelulose, celulose e lignina, análise termogravimétrica e espectros de absorção na região do infravermelho obtidos pela técnica de espectroscopia no infravermelho com transformada de Fourier (FTIR). A pirólise flash analítica foi realizada a 500 °C em equipamento Py-5200 HP-R acoplado a um cromatógrafo à gás (Py-GC/MS). A biomassa das PAC apresenta potencial para processos de conversão termoquímica de energia devido ao seu baixo teor de umidade e cinzas, além de 76,90% de materiais voláteis, densidade aparente de 252,6 kg/m3 e PCS igual a 16,24 MJ / kg. Os produtos da pirólise rápida são principalmente fenóis ou ácidos orgânicos leves derivados da decomposição de polissacarídeos. Os resultados confirmam o potencial das PAC para produzir bio-fenólicos, como o 4-metoxifenol que é um importante ingrediente ativo para despigmentação da pele usado em medicamentos e cosméticos, e como extrato fenólico que pode ser usado como precursor de resinas. Estas aplicações convertem esses resíduos florestais em produtos biológicos para a indústria em uma economia circular verde.

Production of proline and protease with different organic wastes in bacteria (Production proline and protease with organic wastes) / Produção de prolina e protease com diferentes resíduos orgânicos em bactérias (produção de prolina e protease com resíduos orgânicos)

In this study, we investigated the proline and protease production of different bacteria in several organic waste materials. Our aim was to produce proline and protease economically in waste that is abundantly available while reducing its environmental impact. 5 ml of different organic waste materials (OWW: Olive waste water; N.B: Nutrient Broth; EW: Eggshell; PBS: PBS buffer; PLW: Peach leaf wastes; TCW: Turkish coffee wastes; TWW: Tea waste water; WCW: Waste cheese whey; WFO: Waste frying oil) were placed in 10 ml grow tubes, inoculated and incubated for 24 h. Phosphate-buffered saline and 10% solutions of different organic wastes were added. These cultures were subsequently incubated at 37°C for 24 h. Cells were harvested at 24 h for L-proline assay. 1 ml of culture was transferred by pipette into an Eppendorf tube and centrifuged at 14,000 rpm for 20 min at room temperature. Cellular debris was removed by centrifuge and the supernatant was used for proline activity assays. Protease activity was determined using a modified method with casein as the substrate. We found that proline and protease can easily be produced economically using Turkish coffee wastes (TCW), Waste cheese whey (WCW) and Olive waste water (OWW) organic waste. We believe that this study will result in similar research leading to the economical use of these waste materials thus reducing their impact on the environment.

Neste estudo, investigamos a produção de prolina e protease de diferentes bactérias em diversos resíduos orgânicos. Nosso objetivo era produzir prolina e protease economicamente em resíduos que estão disponíveis em abundância, reduzindo seu impacto ambiental. Cinco ml de diferentes materiais de resíduos orgânicos (OWW: resíduos de azeitona; NB: caldo nutriente; EW: casca de ovo; PBS: tampão PBS; PLW: resíduos de folhas de pêssego; TCW: resíduos de café turco; TWW: resíduos de chá; WCW: resíduos de queijo soro de leite; WFO: óleo de fritura residual) foram colocados em tubos de cultivo de 10 ml, inoculados e incubados por 24 horas. Adicionaram-se solução salina tamponada com fosfato e soluções a 10% de diferentes resíduos orgânicos. Essas culturas foram subsequentemente incubadas a 37° C durante 24 h. As células foram colhidas às 24 h para o ensaio de L-prolina. Um ml de cultura foi transferido por pipeta para um tubo Eppendorf e centrifugado a 14.000 rpm, por 20 min, em temperatura ambiente. Os detritos celulares foram removidos por centrifugação e o sobrenadante foi usado para ensaios de atividade de prolina. A atividade da protease foi determinada usando um método modificado com caseína como substrato. Descobrimos que a prolina e a protease podem ser facilmente produzidas economicamente, usando resíduos de café turco (TCW), resíduos de soro de queijo (WCW) e resíduos orgânicos de água de oliva (OWW). Acreditamos que este estudo resultará em pesquisas semelhantes, levando ao uso econômico desses materiais residuais, reduzindo, assim, seu impacto no meio ambiente.

Comparison of nutrient-rich and limited media in the production of biosurfactant by Achromobacter xylosoxidans BP(1)5

Aims@#This study aims to produce Achromobacter biosurfactant in nutrient-rich and nutrient-limited media.@*Methodology and results@#This study conducted fermentation on nutrient-rich and nutrient-limited media using a minimal salt medium (MSM). Dextrose and sodium citrate were used as sole carbon supplemented with 0.5% yeast extract for nutrient-rich media, while nutrient-limited media used molasses and rice straw hydrolysate (RSH) at variations of concentrations of 100 ppm and 200 ppm. The research was performed over 120 h and evaluated from growth response, surface tension and emulsification activity. The study revealed that the best surface tension value was when 2% (w/v) sodium citrate was used as C-source and 0.5% (w/v) yeast extract as N-source, after 72 h upon incubation at 30 °C/120 rpm having 45.45 ± 2.19 mN/m with emulsification activity 24.54 ± 3.42%. Whereas the best result of the nutrient-limited medium was obtained by RSH at a concentration of 200 ppm having 48.86 ± 5.36 mN/m.@*Conclusion, significance and impact of study@#The experiment showed that nutrient-limited medium from rice straw hydrolysate could compete with the nutrient-rich medium. The use of rice straw will contribute to the reduction of biosurfactant production costs and valorisation of agricultural waste.

Production of proline and protease with different organic wastes in bacteria (Production proline and protease with organic wastes) / Produção de prolina e protease com diferentes resíduos orgânicos em bactérias (produção de prolina e protease com resíduos orgânicos)

In this study, we investigated the proline and protease production of different bacteria in several organic waste materials. Our aim was to produce proline and protease economically in waste that is abundantly available while reducing its environmental impact. 5 ml of different organic waste materials (OWW: Olive waste water; N.B: Nutrient Broth; EW: Eggshell; PBS: PBS buffer; PLW: Peach leaf wastes; TCW: Turkish coffee wastes; TWW: Tea waste water; WCW: Waste cheese whey; WFO: Waste frying oil) were placed in 10 ml grow tubes, inoculated and incubated for 24 h. Phosphate-buffered saline and 10% solutions of different organic wastes were added. These cultures were subsequently incubated at 37°C for 24 h. Cells were harvested at 24 h for L-proline assay. 1 ml of culture was transferred by pipette into an Eppendorf tube and centrifuged at 14,000 rpm for 20 min at room temperature. Cellular debris was removed by centrifuge and the supernatant was used for proline activity assays. Protease activity was determined using a modified method with casein as the substrate. We found that proline and protease can easily be produced economically using Turkish coffee wastes (TCW), Waste cheese whey (WCW) and Olive waste water (OWW) organic waste. We believe that this study will result in similar research leading to the economical use of these waste materials thus reducing their impact on the environment.

Neste estudo, investigamos a produção de prolina e protease de diferentes bactérias em diversos resíduos orgânicos. Nosso objetivo era produzir prolina e protease economicamente em resíduos que estão disponíveis em abundância, reduzindo seu impacto ambiental. Cinco ml de diferentes materiais de resíduos orgânicos (OWW: resíduos de azeitona; NB: caldo nutriente; EW: casca de ovo; PBS: tampão PBS; PLW: resíduos de folhas de pêssego; TCW: resíduos de café turco; TWW: resíduos de chá; WCW: resíduos de queijo soro de leite; WFO: óleo de fritura residual) foram colocados em tubos de cultivo de 10 ml, inoculados e incubados por 24 horas. Adicionaram-se solução salina tamponada com fosfato e soluções a 10% de diferentes resíduos orgânicos. Essas culturas foram subsequentemente incubadas a 37° C durante 24 h. As células foram colhidas às 24 h para o ensaio de L-prolina. Um ml de cultura foi transferido por pipeta para um tubo Eppendorf e centrifugado a 14.000 rpm, por 20 min, em temperatura ambiente. Os detritos celulares foram removidos por centrifugação e o sobrenadante foi usado para ensaios de atividade de prolina. A atividade da protease foi determinada usando um método modificado com caseína como substrato. Descobrimos que a prolina e a protease podem ser facilmente produzidas economicamente, usando resíduos de café turco (TCW), resíduos de soro de queijo (WCW) e resíduos orgânicos de água de oliva (OWW). Acreditamos que este estudo resultará em pesquisas semelhantes, levando ao uso econômico desses materiais residuais, reduzindo, assim, seu impacto no meio ambiente.

Production of proline and protease with different organic wastes in bacteria (Production proline and protease with organic wastes)

Abstract In this study, we investigated the proline and protease production of different bacteria in several organic waste materials. Our aim was to produce proline and protease economically in waste that is abundantly available while reducing its environmental impact. 5 ml of different organic waste materials (OWW: Olive waste water; N.B: Nutrient Broth; EW: Eggshell; PBS: PBS buffer; PLW: Peach leaf wastes; TCW: Turkish coffee wastes; TWW: Tea waste water; WCW: Waste cheese whey; WFO: Waste frying oil) were placed in 10 ml grow tubes, inoculated and incubated for 24 h. Phosphate-buffered saline and 10% solutions of different organic wastes were added. These cultures were subsequently incubated at 37°C for 24 h. Cells were harvested at 24 h for L-proline assay. 1 ml of culture was transferred by pipette into an Eppendorf tube and centrifuged at 14,000 rpm for 20 min at room temperature. Cellular debris was removed by centrifuge and the supernatant was used for proline activity assays. Protease activity was determined using a modified method with casein as the substrate. We found that proline and protease can easily be produced economically using Turkish coffee wastes (TCW), Waste cheese whey (WCW) and Olive waste water (OWW) organic waste. We believe that this study will result in similar research leading to the economical use of these waste materials thus reducing their impact on the environment.

Resumo Neste estudo, investigamos a produção de prolina e protease de diferentes bactérias em diversos resíduos orgânicos. Nosso objetivo era produzir prolina e protease economicamente em resíduos que estão disponíveis em abundância, reduzindo seu impacto ambiental. Cinco ml de diferentes materiais de resíduos orgânicos (OWW: resíduos de azeitona; NB: caldo nutriente; EW: casca de ovo; PBS: tampão PBS; PLW: resíduos de folhas de pêssego; TCW: resíduos de café turco; TWW: resíduos de chá; WCW: resíduos de queijo soro de leite; WFO: óleo de fritura residual) foram colocados em tubos de cultivo de 10 ml, inoculados e incubados por 24 horas. Adicionaram-se solução salina tamponada com fosfato e soluções a 10% de diferentes resíduos orgânicos. Essas culturas foram subsequentemente incubadas a 37° C durante 24 h. As células foram colhidas às 24 h para o ensaio de L-prolina. Um ml de cultura foi transferido por pipeta para um tubo Eppendorf e centrifugado a 14.000 rpm, por 20 min, em temperatura ambiente. Os detritos celulares foram removidos por centrifugação e o sobrenadante foi usado para ensaios de atividade de prolina. A atividade da protease foi determinada usando um método modificado com caseína como substrato. Descobrimos que a prolina e a protease podem ser facilmente produzidas economicamente, usando resíduos de café turco (TCW), resíduos de soro de queijo (WCW) e resíduos orgânicos de água de oliva (OWW). Acreditamos que este estudo resultará em pesquisas semelhantes, levando ao uso econômico desses materiais residuais, reduzindo, assim, seu impacto no meio ambiente.

Lipid production by oleaginous microorganisms using food wastes: a review / 生物工程学报

Food wastes are rich in nutrients and can be used for producing useful chemicals through biotransformation. Some oleaginous microorganisms can use food wastes to produce lipids and high value-added metabolites such as polyunsaturated fatty acids, squalene, and carotenoids. This not only reduces the production cost, but also improves the economic value of the products, thus has large potential for commercial production. This review summarized the advances in food waste treatment, with a focus on the lipid production by oleaginous microorganisms using food wastes. Moreover, challenges and future directions were prospected with the aim to provide a useful reference for related researchers.

Microorganisms in the typical anaerobic digestion system of organic solid wastes: a review / 生物工程学报

The facultative anaerobic and strict anaerobic microorganisms enriched and acclimated during the anaerobic digestion process are crucial for the efficiency of the anaerobic digestion system. Most of the problems encountered during running anaerobic digestion processes could be effectively improved via stimulation of microbial metabolic activity. Benefited from the rapid development of microbiome techniques, deeper insights into the microbial diversity in anaerobic digestion systems, e.g. the microbe-microbe interactions and microbe-environment interactions, have been gained. A complex and intricate metabolic network exists in the anaerobic digestion system of solid organic wastes. However, little is known about these interactions and the underlying mechanisms. This review briefly summarized the representative interactions between microbial communities during anaerobic digestion process discovered to date. In addition, typical issues encountered during the anaerobic digestion of solid organic wastes and how microbes can tackle and alleviate these issues were discussed. Finally, future priorities on microbiome research were proposed based on present contribution of microbiome analysis in anaerobic digestion system.

Unveiling Xylanolytic Enzymes Production of Talaromyces wortmannii DR49 on Industrial Agro Wastes

Abstract Xylan degradation is an important step in different industries, such as in biorefinery for biomass hydrolysis. Talaromyces wortmannii is a known fungus due to second metabolite production but only few works showed the xylanolytic potential of this fungus. In this way, the aim of this study was to evaluate the production of xylanolytic enzymes from T. wortmannii DR49 on industrial agro wastes. Cultivation in shake flask showed highest xylanase titration (10.3 U/mL; 9.5 U/mL) for wheat bran (WB) and hydrothermal pretreated sugar cane bagasse (HB); in β-xylosidase production WB and xylose were the best carbon sources (0.57 U/mL; 0.34 U/mL) respectively. STR cultivation revealed that 29°C and pH 6.0 were the best conditions for xylanase (14.5 U/mL) and β-xylosidase (1.7 U/mL) production. T. wortmannii DR49 showed to be a potential candidate for xylanolytic enzymes production using agro wastes in bioreactors, which has never been previously reported in this fungus.

Tecnologic Development on Pleurotus Cultivation: Specific Practices Used in Brazil

HIGHLIGHTS P. ostreatus and P. sapidus are the most productive species under the evaluated conditions. Different growing systems are suitable for the production of P. ostreatus var. Florida. Temperature control level affects differently the P. ostreatus var. Florida isolates. Environmental and strain factors affect yield and production parameters of P. ostreatus var. Florida.

Abstract In Brazil, Pleurotus is the most important mushroom produced especially P. ostreatus var. Florida. In this country as in many others, the great potential for mushroom cultivation remains unexplored. Therefore, it is very important to develop new studies that allow optimizing its production. The aims of the manuscript were: i) to evaluate the productivity of six different species of Pleurotus (P. citrinopileatus; P. djamor; P. ostreatus; P. ostreatus var. Florida; P. pulmonarius; P. sapidus); ii) to measure the effect of three different environmental conditions during cultivation of three isolates of P. ostreatus var. Florida. As results, P. ostreatus and P. sapidus were the most productive isolates under the evaluated conditions. Different environments produced variable effects according to the P. ostreatus var. Florida isolates, being possible to observe a highly plastic strain (POF 02/18), a highly sensitive strain (POF 03/18) and a strain with variable responses (POF 01/18).

Fatores que influenciam a produção de ácidos carboxílicos a partir de resíduos agroindustriais / Factors that influence the production of carboxylic acids from agroindustrial wastes

RESUMO Os ácidos carboxílicos (ACs) são componentes químicos de base produzidos principalmente a partir da plataforma petroquímica. Entretanto, em razão de impactos ambientais, risco de escassez e elevados preços do petróleo, a produção biológica de AC por meio da digestão anaeróbia de recursos renováveis de baixo custo, como resíduos orgânicos das atividades agroindustriais, vem recebendo atenção crescente. Nesse contexto, o presente artigo objetiva discutir o processo de digestão anaeróbia aliada ao alongamento de cadeia carboxílica, a fim de orientar tomadas de decisão, como, por exemplo, escolha do substrato e definição das condições ambientais e dos parâmetros operacionais. Em geral, as condições que apresentam melhor viabilidade para produção de ácidos carboxílicos de cadeia média (ACCM) são: pH próximo a 6,0; temperatura mesofílica, já que oferece maior estabilidade operacional e os rendimentos obtidos em condições termofílicas não se mostram muito superiores; pressão parcial de hidrogênio (PPH) próxima a 10-5 aliada à limitação de CO2; tempo de residência celular (θc) inferior a 5 dias; e tempo de detenção hidráulica (TDH) baixo para sistemas contínuos. Uma das configurações mais promissoras é o uso de sistemas de dois estágios utilizando baixo pH como inibidor da metanogênese e extração de ACs em linha, para evitar a inibição devido à toxicidade dos produtos.

ABSTRACT Carboxylic acids (CA) are basic chemical components produced mainly through the petrochemical platform. However, due to environmental impacts, risk of scarcity and high petroleum prices, organic production of CA from the anaerobic digestion of low-cost renewable resources, such as organic wastes from agro-industrial activities, has been receiving increasing attention. In this context, the present paper aims to discuss the process of anaerobic digestion allied to the carboxylic chain elongation process, in order to guide decision making such as substrate choice, definition of environmental conditions, and operational parameters. In general, the conditions that present the best viability for MCCA production are: pH close to 6.0; mesophilic temperature since it offers greater operational stability and the yields obtained under thermophilic conditions are not much higher; hydrogen partial pressure (HPP) close to 10-5 combined with CO2 limitation; solids retention time (θc) < 5 days and low hydraulic retention time (HRT) for continuous systems. One of the most promising configurations is the use of two-stage systems using low pH as a methanogenesis inhibitor and inline CA extraction to avoid inhibition due to product toxicity.

Condições socioeconômicas, de saúde e hábitos de vida dos catadores de material reciclável / Socio-economic, health and lifestyle conditions of recycled materials collectors

O presente estudo objetivou analisar as condições socioeconômicas, de saúde e hábitos de vida dos catadores de material reciclável em duas cooperativas no município de Guanambi - Bahia, Brasil. Trata-se de uma pesquisa de natureza descritiva, analítica e exploratória e todos os catadores foram entrevistados por meio de um questionário semiestruturado. Houve predominância da faixa etária entre 30 a 58 anos (80%) (p = 0,007), pardos (55%) (p = 0,02), casados (45%) (p = 0,02), com 2 a 3 filhos (55%) (p = 0,03). Os catadores informaram que começaram a trabalhar com menos de 18 anos (85%) (p = 0,002), e recebem uma renda mensal de até 1 salário mínimo com o trabalho de reciclagem (75%) (p = 0,02). Em relação às condições de saúde, 75% afirmaram que possuem Equipamentos de Proteção Individual (EPI) (p = 0,02), no entanto 80% (p = 0,02) não utilizaram o EPI e não consideram que seu trabalho pode provocar problema de saúde (75%) (p = 0,02). Percebe-se que os catadores de material reciclável precisam intensificar o uso dos EPI's, visando minimizar os danos com a saúde, além de melhorias na renda e na qualidade de vida.

The social, economic, health and lifestyle conditions of collectors of recyclable material from two coops in Guanambi, Brazil, are analyzed through a descriptive, analytic and exploratory research. All collectors were interviewed by a half-structured questionnaire. Predominant age bracket lay between 30 and 58 years (80%) (p= 0.007), brown colored (55%) (p= 0.02), married (45%) (p= 0.02), with 2 - 3 children (55%) (p= 0.03). Collectors informed that they started work when they were under 18 years old and received a month wage of up to one minimum wage, by collecting recycling material (75%) (p= 0.02). With regard to health conditions, 75% stated that had individual protection equipment (EPI) (p= 0.02), but 80% (p= 0.02) failed to use it, insisting that their work does not cause health problems (75%) (p= 0.02). It should be underscored that collectors should improve their earnings and life quality and use their equipments to minimize health risks.

Antimicrobial resistance: Causes and impact on public health in developed and developing countries

Antibiotic-resistance (AR) has become an alarming issue, posing threats to public health in terms of mortality and economic loss. The drivers of AR include environmental contamination from varied sources, ultimately making its way into our drinking water and food. Other factors include reckless use of antibiotics by the uneducated health workers, unhygienic situation of the hospitals, overconsumption and careless discharge of medicines by the general populace, injudicious use of antibiotics in the livestock, and indiscriminate disposal of untreated pharmaceutical wastes into the municipal water have caused several health hazards, such as “AR in infants,” respiratory disorders, and cancer. Furthermore, the sewage treatment process itself augments the antimicrobial resistance crisis. Hence, it is an alarming issue which must be taken care at the global level as well as the national level.

Technical guideline for disinfection of wastewater and wastes of medical organizations during the novel coronavirus pneumonia(NCP)outbreak / 中华预防医学杂志

This technical guideline is applicable to disinfection of wastewater and wastes of medical organizations during the novel coronavirus pneumonia (NCP) outbreak. The purpose of the guideline is to eliminate various pathogenic bacteria, fungi, and viruses, effectively block the virus transmission pathways, and guide medical organizations to carry out the prevention and control of NCP.

Evaluation of the physio-chemical properties and microbial load of the soil polluted with coffee processing wastes

@#Aim: The study was designed to evaluate the physio-chemical properties and microbial load of the soil polluted with coffee processing wastes such as coffee husk and coffee pulp. Methodology and results: A total of ten soil samples were taken from three taluks of Coorg district of Karnataka, India. Out of which five soil samples were taken from places where the coffee processing wastes were dumped as landfills. The other five soil samples were taken from places free from coffee processing wastes which represent the control soil samples. The physical and chemical properties of the soil were measured using standard protocols. The highlight of the study was quantification of chemicals of ecotoxicological concern such as caffeine, polyphenols and tannin in soil samples. The identification and enumeration of soil bacteria, fungi, actinomycetes, yeast and plant growth promoting microorganisms were also done. The pollution with the coffee processing wastes make the soil acidic. The concentration of chemicals of ecotoxicological concern such as caffeine, polyphenols and tannins were significantly high in polluted soil. The colony forming units of plant growth promoting microorganism were declined significantly in the polluted soil. Instead of all these detrimental factors, the organic carbon, nitrogen, potassium, phosphorus and micronutrient content of the polluted soil was significantly high. Conclusion, significance and impact of study: This study revealed the fact that the unscientific disposal of coffee processing wastes as landfill make the soil less fertile, damage the normal microbial diversity of the soil and would cause severe pollution problems.

Enzymes modulation by dried grape pomace from the manufacture of wines and juices

The processing of grapes for the manufacture of juices and wines, generates large quantities of by-products rich in metabolites with antioxidant, antimicrobial, anti-inflammatory and cicatrizing activities. The high homology between human enzymes and snake venoms makes the latter valuable laboratory tools for the study of pathophysiological processes. Proteases and phospholipases A2 act in processes related to hemostasis and inflammatory response. Thus, in this work, dried pomace obtained from grape (Isabel, Niagara, Bordô, BRS Violeta and Blend cultivars) processing were evaluated on phospholipase, proteolytic, hemolytic and thrombolytic activities induced by snakes venoms and the content of phenolic compounds and minerals was evaluated. The dried pomace exerted inhibitory and potentiating actions in all analyzed activities. The enzymatic modulators present in the evaluated dried pomace have potential for therapeutic use, although their broad characterization is still necessary, in order to define adequate amounts and formulations to obtain efficacy and safety in their use.