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Objective: To investigate the clinical clues to differentiate between Dravet syndrome (DS) and febrileseizures plus (FS+). Methods: From September 2001 to March 2014, 44 consecutive patients whowere diagnosed with DS or FS+, were recruited. We retrospectively analyzed the characteristics of thefirst seizure and findings of patients exhibiting seizures during hot water immersion at the first visit.Comparisons between the two groups were analyzed. Results: Thirty-two DS and 12 FS+ patientswere enrolled. The most prevalent body temperature classification in the DS group was afebrile(43.8% vs. 25%, p=0.001), followed by 37-37.9oC (31.3% vs. 8.3%, p=0.02), and that in the FS+group was at 39oC or above (33.3% vs. 9.4%, p=0.001). The most prevalent seizure type in the DSgroup was focal motor seizures (43.8% vs. 25%, p=0.001), followed by alternating hemiconvulsiveseizures (12.5% vs. 0%, p=0.005), and that in the FS+ group was generalized clonic and/or tonic-clonicseizures (83.3% vs. 37.5%, p=0.002). Compared with the FS+ group, there was a greater prevalenceof vaccination-related seizure as the first presenting feature among the DS patients (46.9% vs. 8.3%,p<0.001). During hot water immersion, myoclonic seizure was seen significantly in the DS group(46.4% vs. 25.5%, p=0.013).Conclusions: Afebrile and mild body temperature variation below 38oC, focal motor seizure oralternating hemiconvulsive seizure types, and vaccination-related first seizure were found to be cluesfor highly suspected DS.
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The world is culturally endowed with various forms of healing practices having rich medical wisdom of immense importance. One of such pharmacognostically indispensable medicinal plant is Embelia tsjeriam-cottam A. DC. The active principle of E. tsjeriam-cottam is embelin, possessing a range of pharmacognostic activities including anti-cancer, antioxidant, antiinflammation, antibacterial and analgesic effects. Embelin in fruits of E tsjeriam-cottam was extracted using water bath method. For extraction of embelin from fruits of E. tsjeriamcottam, methanol and chloroform were used as solvents. Fruits were collected from five different agro-climatic zones of Odisha. Comparative estimation of embelin was done through spectrophotometer and High Performance Liquid Chromatographic (HPLC) methods. Samples extracted with chloroform and methanol showed embelin content in a range of 2.13- 0.29% dry wt., and 0.95-0.28% dry wt. respectively using spectrophotometer. In case of HPLC analysis, samples extracted in chloroform and methanol showed embelin content in a range of 1.86-0.27% dry wt., and 0.875-0.26% dry wt. respectively. However the water bath method, used as alternative method for extraction, proved to be less time consuming, costeffective in extracting a pretty good amount of embelin as compared to the conventional (soxhlet) methods of extraction.
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Objective To investigate and evaluate the comparison results of five items of hepatitis B in ELISA by using water bath box and rapid incubation .Methods 92 patients with hepatitis B in our hospital from June 2012 to June 2013 were randomly se‐lected and 184 cases of healthy physical examination were randomly selected as the controls .The water bath and rapid incubation were adopted to conduct ELISA for simultaneously detecting 5 items of hepatitis B in samples .Results The results of two groups had higher positive coincidence rate .The average OD value of specimens in the water bath was higher .But the specimen S/CO value in the fast average incubation was higher ,its co value was higher than that of the water bath .Conclusion The rapid incubation can be introduced for improving the work efficiency in the daily work ,but the specimen with weak reactiveresult needs to be re‐de‐tected by using the water bath and making records .The fast incubation device is best for only use of the HBsAg reaction ,because the other four reaction process time is shorter ,and there are some non‐conformity results .
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Objectives: Peripheral artery disease (PAD) is a major health problem; however, no satisfactory intervention is available for its treatment. This study was undertaken to investigate the effects as well as mechanisms of the CO2-enriched water bath (CEWB) treatment on blood flow in the ischemic hind limb. Experimental Model: For inducing PAD, the femoral artery was occluded for 5 weeks in rats. The animals were treated with or without CEWB at 37°C for 4 weeks (20 min daily; 5 days per week) starting one week after the artery occlusion. CEWB was prepared by using Carbothera (Mitsubishi Rayon Engineering Tokyo). The blood flow was measured by Pulse Wave Doppler Ultrasound technique before and after the ligation as well as at the end of 4 weeks treatment. The angiogenesis (formation of new blood vessels) in the skeletal muscle was studied by histological examination. Results: The peak, mean or minimal blood flow was not detected in the untreated ischemic hind limb animals due to arterial ligation. However, the values for blood flow were about 50% of the control values upon treatment with CEWB; 67% of the ligated animals showed positive blood flow by CO2 treatment. Morphological examination of the treated ischemic skeletal muscle revealed a 3-fold increase in small artery count. Although plasma triglycerides were decreased and plasma NO concentration was increased in the ischemic animals, CEWB treatment produced no effects on these parameters. No mortality or changes in body wt, heart rate and plasma glucose, cholesterol or high density lipoproteins were seen in the control and experimental animals. Conclusion: This study demonstrates the beneficial effect of CEWB treatment on blood flow in hind limb PAD. Furthermore, it is suggested that this beneficial action of CO2 therapy may be due to the formation of new blood vessels in the ischemic skeletal muscle.
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<b>Objectives:</b> Peripheral artery disease (PAD) is a major health problem; however, no satisfactory intervention is available for its treatment. This study was undertaken to investigate the effects as well as mechanisms of the CO<sub>2</sub>-enriched water bath (CEWB) treatment on blood flow in the ischemic hind limb.<BR><b>Experimental Model: </b>For inducing PAD, the femoral artery was occluded for 5 weeks in rats. The animals were treated with or without CEWB at 37°C for 4 weeks (20 min daily; 5 days per week) starting one week after the artery occlusion. CEWB was prepared by using Carbothera (Mitsubishi Rayon Engineering Tokyo). The blood flow was measured by Pulse Wave Doppler Ultrasound technique before and after the ligation as well as at the end of 4 weeks treatment. The angiogenesis (formation of new blood vessels) in the skeletal muscle was studied by histological examination.<BR><b>Results:</b> The peak, mean or minimal blood flow was not detected in the untreated ischemic hind limb animals due to arterial ligation. However, the values for blood flow were about 50% of the control values upon treatment with CEWB; 67% of the ligated animals showed positive blood flow by CO<sub>2</sub> treatment. Morphological examination of the treated ischemic skeletal muscle revealed a 3-fold increase in small artery count. Although plasma triglycerides were decreased and plasma NO concentration was increased in the ischemic animals, CEWB treatment produced no effects on these parameters. No mortality or changes in body wt, heart rate and plasma glucose, cholesterol or high density lipoproteins were seen in the control and experimental animals.<BR><b>Conclusion:</b> This study demonstrates the beneficial effect of CEWB treatment on blood flow in hind limb PAD. Furthermore, it is suggested that this beneficial action of CO<sub>2</sub> therapy may be due to the formation of new blood vessels in the ischemic skeletal muscle.
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Purpose To test the virus inactivation effect of water bath method at 60 ℃ for 10 hours and alcohol treatment for 3 hours which was used in the production of urinary trypsin inhibitor(UTI).Methods Sindbis virus,Pseudorabies virus(PRV) and poliovirus1(PV1) were used as indicated viruses in this test.After being added separately into the UTI raw material in 10% proportion,the viruses were treated with water bath at 60 ℃ for 10 hours and alcohol for 3 hours and then the samples of UTI were taken to inoculate the cell line for assay of cytopathic effect.Results The water bath at 60 ℃ for 10 hours could inactive Sindbis,PRV and PV1 in more than(6.503±0.102)LgTCID_(50),(6.42±0.158) LgTCID_(50) and(6.587±0.061)LgTCID_(50) respectively,and alcohol treatment for 3 hours could inactive Sindbis,PRV and PV1 in more than(5.88±0.204)LgTCID_(50),(6.378±0.268)LgTCID_(50) and(5.963±0.118) LgTCID_(50) respectively.No cytopathic effect was found in the cell line which was inoculated with treated samples after blind passage for three generations.Conclusion The water bath method at 60 ℃ for 10 hours and alcohol treatment for 3 hours which were used in the production of UTI had good effects on virus inactivation and the inactivation efficiency on Sindbis,PRV and PV1 was more than 6 LgTCID_(50)/mL.
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Medical thermostat water bath cauldron is widely used in clinic,but its temperature control is not accurate.This paper presents a digitalized revolution on medical thermostat water bath cauldron.
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OBJECTIVE:To establish a method for simultaneous determination of ferulic acid(FA)and TMP in serum sample.METHODS:After deprotein with boiling water-bath,RP-HPLC was performed on ODS C 18 column(250mm?4.6mm)with methanol-water(containing0.1%acetic acid)=8∶17as the mobile phase and UV detection at280nm.RESU LTS:The calibration curve of FA was linear within the range of30.2～70.8?g/ml,Y=57.51+413.12X,r=0.9974;the curve of TMP was linear within the range of10.1～50.7?g/ml,Y=41.32+308.64X,r=0.9922.CONCLUSION:Compared with the traditional extraction,the method is simple,rapid and sensitive,and can be used for simultaneous determination of the contents of active compositions in Chinese materia medica.